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Annals of Microbiology

, Volume 69, Issue 3, pp 253–265 | Cite as

Antimicrobial activity and diversity of bacteria associated with Taiwanese marine sponge Theonella swinhoei

  • Jimmy KuoEmail author
  • Yu-Ting Yang
  • Mei-Chin Lu
  • Tit-Yee Wong
  • Ping-Jung Sung
  • Yung-Sen Huang
Original Article
  • 111 Downloads

Abstract

Marine sponges often rely on other epiphytes for protection from harmful predators. To understand the diversity and antimicrobial activity present among epiphytic bacteria isolated from marine sponge. We used both the 16S rRNA tag pyrosequencing method and the culture-based method to investigate the bacterial communities of Theonella swinhoei collected off the shore of southern Taiwan. Eight-hundred and eighteen operational taxonomic units (OTUs; 97% sequence similarity) were identified from 23,700 sponge-derived sequence tags. The bacteria associated with T. swinhoei were found to be highly diverse—as many as 12 different phyla of bacteria were identified. However, in terms of population evenness, the community was dominated by two phyla—Acidobacteria (71.54%) and Chloroflexi (19.60%). A total of 700 bacterial strains were isolated and cultured from samples of the sponge T. swinhoei. Within these culturable strains, only 12% were Actinomycetes. Despite the low percentage of Actinobacteria from the samples, among the 51 strains of culturable bacteria that showed high antimicrobial activity, a great majority (62%) were Actinomycetes (30 strains of Streptomyces and 1 strain each of Micromonospora and Brevibacterium). The remaining isolates that produced antimicrobial compounds were Gammaproteobacteria (10 strains of Pseudoalteromonas) and Firmicutes (8 and 1 strains of Bacillus and Paenibacillus, respectively). We speculated that many more Actinomycetes are yet to be isolated from T. swinhoei microbiota. Advanced techniques, such as high-throughput culture and culturome, should allow the isolation and purification of these medically important groups of bacteria from sponge.

Keywords

Sponge-associated bacteria Antimicrobial activity Cytotoxic activity Metagenomic analysis 454 pyrosequencing 

Notes

Acknowledgements

This work was supported by intramural funding from the National Museum of Marine Biology and Aquarium.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Supplementary material

13213_2018_1414_MOESM1_ESM.xlsx (54 kb)
Supplementary File 1 Taxonomy assignments of the bacterial community associated with marine sponge Theonella swinhoei at different levels. (XLSX 53.9 kb)

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Copyright information

© Università degli studi di Milano 2019

Authors and Affiliations

  1. 1.Department of Planning and ResearchNational Museum of Marine Biology and AquariumPingtungTaiwan
  2. 2.Graduate Institute of Marine BiologyNational Dong Hwa UniversityPingtungTaiwan
  3. 3.Department of Biological SciencesUniversity of MemphisMemphisUSA
  4. 4.Department of Life SciencesNational University of KaohsiungKaohsiungTaiwan

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