Characterization of a biosurfactant-producing Leclercia sp. B45 with new transcriptional patterns of alkB gene
- 24 Downloads
To investigate the hydrocarbon-degrading ability, biosurfactant-producing capacity, and alkane monooxygenase system of Leclercia spp. A bacterial strain classified as Leclercia sp. B45 was isolated, and its biosurfactant-producing capacity, hydrocarbon-degrading ability, and alkane hydroxylase (alkB) gene transcriptional patterns were evaluated by TLC, FTIR, GC-MS, and RT-qPCR, respectively. Strain B45 showed active biosurfactant-producing ability, which was preferentially induced by C16. The extracted biosurfactant tolerated a wide range of salinity, pH, and temperature. The degradation rate of n-decane (C10), n-hexadecane (C16), and octacosane (C28) by strain B45 could reach 92.6%, 94.1%, and 67.8%, respectively. Furthermore, the alkB transcription levels in the strain B45 with C10, C16, or C28 as a carbon source were distinctly higher than those of the control group during the late exponential and stationary phases. The relative alkB transcript copy number decreased with the increase in alkane chain length, which is consistent with B45 strain biodegradation kinetics. Leclercia sp. B45 showed excellent n-alkane degradation performance and biosurfactant-producing capacity. Meanwhile, the alkB gene in Leclercia sp. B45 is likely to represent a novel gene, whose transcription level was significantly upregulated when induced by n-alkane. These results provide new insights into alkane metabolism mechanism in Leclercia sp. B45.
KeywordsLeclercia sp. Alkane alkB gene Biosurfactant
Yiying Shuai and Hanghai Zhou conducted the experiments and the analysis, and drafted the initial version of the manuscript. Qinglin Mu helped write the molecular biology section. Dongdong Zhang helped modify the whole paper. Ning Zhang helped the analysis of molecular biology data. Jingchun Tang helped write the discussion section of the manuscript as well as constructing the phylogenetic tree for the bacterial strains. Chunfang Zhang conceived, designed, and supervised the research work. All authors read and approved the final manuscript. This manuscript has not been published or presented elsewhere in part or entirety and is not under consideration by another journal. The statements provided by all authors are true.
This study was supported by the China Association of Marine Affairs (no. 2016AB033), by the Open Foundation from Fishery Sciences in the First-Class Subjects of Zhejiang (no. 20160006), and by XinJiang Keli New Technology Development Co., Ltd (K17-529102-004, K18-529102-014).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
This article does not contain any studies with human participants performed by any of the authors.
This study does not require informed consent.
- Abdel-Megeed A (2013) Potential degradation of certain alkanes by Pseudomonas frederiksbergensis. J Pure Appl Microbiol 7(3):13–21Google Scholar
- Alan GM, Rodgers RP (2004) Petroleomics: the next grand challenge for chemical analysis. Acc Chem Res 35(15):53–59Google Scholar
- Asadollahi L, Salehizadeh H, Yan N (2016) Investigation of biosurfactant activity and asphaltene biodegradation by Bacillus cereus. J Polym Environ 24(2):119–128Google Scholar
- Ayed HB, Jridi M, Maalej H, Nasri M, Hmidet N (2014) Characterization and stability of biosurfactant produced by Bacillus mojavensis A21 and its application in enhancing solubility of hydrocarbon. J Chem Technol Biotechnol 89(7):1007–1014Google Scholar
- Chioma O, Ogechukwu M, Bright O, Simon O, Chinyere AF (2013) Isolation and characterization of biosurfactants producing bacteria from oil polluted soil. J Natl Sci Res 3(5):119–122Google Scholar
- Csutak O, Stoica I, Vassu T (2012) Evaluation of production, stability and activity of biosurfactants from yeasts with application in bioremediation of oil-polluted environment. Rev Chim 63(10):973–977Google Scholar
- Dehghan G, Dehghan NA, Moshafi MH, Ahmadi-Afzadi M (2010) Investigating the effects of various additives on surface activity and emulsification index of biosurfactant resulting from broth media of Bacillus subtilis PTCC 1023. Afr J Microbiol Res 4(4):1981–1990Google Scholar
- Eastcott L, Wan YS, Mackay D (1988) Environmentally relevant physical-chemical properties of hydrocarbons: a review of data and development of simple correlations. Oil Chem Pollut 4(3):191–216Google Scholar
- Figueiredo CR, Matsuo AL, Pereira FV, Farias CF, Girola N (2014) Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo. Pharmacogn Mag 10(Suppl 2):S363–S376PubMedPubMedCentralGoogle Scholar
- Habib S, Johari WLW, Shukor MY, Yasid NA (2017) Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP. Bioremediation Sci Technol Res 5(2):13–16Google Scholar
- Hassanshahian M, Yakimov MM, Denaro R, Genovese M, Cappello S (2014) Using real-time PCR to assess changes in the crude oil degrading microbial community in contaminated seawater mesocosms. Int Biodeterior Biodegrad 93(93):241–248Google Scholar
- Hayaishi O, Katagiri M, Rothberg S (1955) Mechanism of the pyrocatechase reaction. J Am Chem Soc 77(20):5450–5451Google Scholar
- Joy S, Rahman PKSM, Sharma S (2017) Biosurfactant production and concomitant hydrocarbon degradation potentials of bacteria isolated from extreme and hydrocarbon contaminated environments. Chem Eng J 317:232–241Google Scholar
- Kumar AP, Janardhan A, Viswanath B, Monika K, Jung JY, Narasimha G (2016) Evaluation of orange peel for biosurfactant production by Bacillus licheniformis and their ability to degrade naphthalene and crude oil. Biotech 6:43–52Google Scholar
- Laorrattanasak S, Rongsayamanont W, Khondee N, Paorach N, Soonglerdsongpha S, Pinyakong O, Luepromchai E (2016) Production and application of gordonia westfalica GY40 biosurfactant for remediation of fuel oil spill. Water Air Soil Pollut 227:325–337Google Scholar
- Li X, Fan F, Zhang K, Chen B (2018) Biosurfactant enhanced soil bioremediation of petroleum hydrocarbons: design of experiments (DOE) based system optimization and phospholipid fatty acid (PLFA) based microbial community analysis. Int Biodeterior Biodegrad 132:216–225Google Scholar
- Luna JM, Rufino RD, Campos-Takaki GM, Sarubbo LA (2012) Properties of the biosurfactant produced by Candida Sphaerica cultivated in low-cost substrates. Int Conf Ind Biotechnol 27:67–72Google Scholar
- Perchet G, Sangely M, Goñi M, Merlina G, Revel J, Pinelli E (2008) Microbial population changes during bioremediation of nitroaromatic- and nitramine-contaminated lagoon. Int Biodeterior Biodegrad 61:304–312Google Scholar
- Shao Z (2011) Trehalolipids. In: Soberon-Chavez G (ed) Biosurfactant:from GenestoApplication, Springer Berlin Heidelberg, p 121–143Google Scholar
- Smith THM, Balada SB, Witholt B, van Beilen JB (2002) Functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria. J Bacteriol 184(6):1733–1742Google Scholar
- Soudi MR, Nasr S, Attaran B, Mehrnia MR, Sarrafzadeh MH (2010) Stability of biosurfactant produced by native strain of bacillus subtilis in variation of environmental conditions. Iran J Biol 2:172–178Google Scholar
- Souza EC, Vessoni-Penna TC (2014) Biosurfactant-enhanced hydrocarbon bioremediation: an overview. Int Biodeterior Biodegrad 89(2):88–94Google Scholar
- van Beilen JB, Witholt B (2005) Diversity, function, and biocatalytic applications of alkane oxygenases. In: Ollivier B, Magot M (eds) Petroleum microbiology. ASM Press, Washington, DC, pp 259–276Google Scholar
- van Beilen JB, Li Z, Duetz WA, Smits THM, Witholt W (2003) Diversity of alkane hydroxylase systems in the environment. Oil Gas Sci Technol 58(58):427–440Google Scholar
- Zhang K, Sun Y, Cui Z, Yu D, Zheng L (2017) Periodically pilled-oil input as a trigger to stimulate the development of hydrocarbon-degrading consortia in a beach ecosystem. Sci Rep 7(1):1–9Google Scholar
- Zhou H, Chen J, Yang Z, Qin B, Li Y (2015) Biosurfactant production and characterization of Bacillus sp. ZG0427 isolated from oil-contaminated soil. Ann Microbiol 65(4):2255–2264Google Scholar