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Annals of Microbiology

, Volume 69, Issue 1, pp 41–49 | Cite as

Microbial community dynamic in tomato fruit during spontaneous fermentation and biotechnological characterization of indigenous lactic acid bacteria

  • Aisse Bah
  • Raoudha Ferjani
  • Imene Fhoula
  • Yosra Gharbi
  • Afef Najjari
  • Abdellatif Boudabous
  • Hadda Imene OuzariEmail author
Original Article
  • 61 Downloads

Abstract

The present work aimed to study the microbial dynamics in tomato fruits under spontaneous fermentation process and the biotechnological properties of lactic acid bacteria (LAB) for future starter culture formulation. The isolation of native microbial species was performed using diverse specific media. Microbial identification was performed using the variability analysis and sequencing of ribosomal DNA-amplified fragments. Moreover, LAB (n = 85) were evaluated for several physiological and technological characteristics, including salinity and temperature tolerance, esculin and arginine hydrolysis, carbohydrate fermentation, exopolysaccharide production, and antagonistic activity. As well, lycopene, flavonoid, and antioxidant compounds were determined in fermented tomato samples. Bacterial isolates were assigned to ten bacterial genera, namely, Microbacterium, Bacillus, Staphylococcus, Pantoea, Flavobacterium, Enterobacter, and Citrobacter including three genera of LAB: Lactobacillus, Leuconostoc, and Enterococcus. Moreover, the amplification of ITS1-5.8S-ITS2 regions allowed the detection of Aspergillus fumigatus and three species of yeast: Candida carpophila, Meyerozyma caribbica, and Wickerhamomyces onychis. During the spontaneous fermentation, the majority of spoilage bacteria and fungi completely disappeared after the third week, whereas LAB and yeast remain until the end of fermentation. LAB exhibited important technological features and high antibacterial activity against human and food-borne pathogenic bacteria. Furthermore, LAB produced various antioxidants for fermented food products. Promising results of this study allowed the identification of the major encountered taxa during spontaneous fermentation of tomato and underline the importance of LAB as a starter culture to achieve microbiologically safe products providing prolonged stability and flavor of vegetable-derived foods.

Keywords

Microbial dynamics Spontaneous fermentation Lactic acid bacteria Biotechnological properties 

Notes

Acknowledgements

The authors thank the Tunisian Ministry of Higher Education and Scientific research in the ambit of the laboratory project LR03ES03.

Funding

Financial support from the Tunisian Ministry of Higher Education and Scientific Research in the ambit of the laboratory research project LR03ES03.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Abed TA (2013) Evaluation of methods for the extraction and purification of DNA of cultured Lactobacillus colony isolated from dairy products. Int J Appl Microbiol Biotechnol Res 1:20–25Google Scholar
  2. Aguilar C, Consuelo, Bernadette K (2011) Antagonistic effect of Lactobacillus strains against Escherichia coli and Listeria monocytogenes in milk. J Dairy Res 78:136–143CrossRefGoogle Scholar
  3. Alegre I, Viñas I, Usall J, Anguera M, Abadias M (2011) Microbiological and physicochemical quality of fresh-cut apple enriched with the probiotic strain Lactobacillus rhamnosus GG. Food Microbiol 28:59–66CrossRefGoogle Scholar
  4. Atoui A, El Khoury A, Kallassy M, Lebrihi A (2012) Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize. Int J Food Microbiol 154:9–65CrossRefGoogle Scholar
  5. Cerning J, Bouillanne C, Landon M, Desmazeaud M (1992) Isolation and characterization of exopolysaccharides from slime-forming Mesophilic lactic acid Bacteria. J Dairy Sci 75:692–699CrossRefGoogle Scholar
  6. Daffonchio D, Borin S, Frova G, Manachini PL, Sorlini C (1998) PCR fingerprinting of whole genomes: the spacers between the 16s and 23s rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniforrnis. Int J Syst Bacteriol 48:107–1 16CrossRefGoogle Scholar
  7. De Almeida Júnior WLG, Ferrari IS, de Souza JV, da Silva CDA, da Costa MM, Dias FS (2015) Characterization and evaluation of lactic acid bacteria isolated from goat milk. Food Control 53:96–103CrossRefGoogle Scholar
  8. Di Cagno R, Coda R, De Angelis M, Gobbetti M (2013) Exploitation of vegetables and fruits through lactic acid fermentation. Food Microbiol 33:1–10CrossRefGoogle Scholar
  9. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Annal Chem 28:350–356CrossRefGoogle Scholar
  10. Dufossé L (2006) Microbial production of food grade pigments. Food Technol Biotechnol Rev 44(3):313–321Google Scholar
  11. Ferjani R, Marasco R, Rolli E, Cherif H, Cherif A, Gtari M, Boudabous A, Daffonchio D, Ouzari HI (2015) The date palm tree Rhizosphere is a niche for plant growth promoting Bacteria in the oasis ecosystem. Biomed Res Int 2015:1–10CrossRefGoogle Scholar
  12. Fhoula I, Najjari A, Turki Y, Jaballah S, Boudabous A, Ouzari H (2013) Diversity and antimicrobial properties of lactic acid bacteria isolated from Rhizosphere of olive trees and desert truffles of Tunisia. BioMed Res Int 405708Google Scholar
  13. Gebbers JO (2007) Atherosclerosis, cholesterol, nutrition, and statins a critical. Ger Med Sci Rev 5:1–11Google Scholar
  14. Harris LJ (1998) The microbiology of vegetable fermentations. In: Wood BJB (eds) Microbiology of fermented foods. Springer, BostonGoogle Scholar
  15. Javanmardi J, Kubota C (2006) Variation of lycopene, antioxidant activity, total soluble solids and weight loss of tomato during postharvest storage. Postharvest Biol Technol 4:151–155CrossRefGoogle Scholar
  16. Kachouri F, Ksontini H, Kraiem M, Setti K, Mechmeche M, Hamdi M (2015) Involvement of antioxidant activity of lactobacillus plantarum on functional properties of olive phenolic compounds. J Food Sci Technol 52(12):7924–7933CrossRefGoogle Scholar
  17. Khedid K, Faid M, Mokhtari A, Soulaymani A, Zinedine A (2009) Characterization of lactic acid bacteria isolated from the one humped camel milk produced in Morocco. Microbiol Res 164:81–91CrossRefGoogle Scholar
  18. Landete JM, Pardo I, Ferrer S (2007) Tyramine and phenyl ethylamine production among lactic acid bacteria isolated from wine. Int J Food Microbiol 115:364–368CrossRefGoogle Scholar
  19. Lee SH, Jung JY, Lee SH, Jeon CO (2011) Complete genome sequence of Leuconostoc kimchii strain C2, isolated from Kimchi. J Bacteriol 193(19):5548CrossRefGoogle Scholar
  20. Lin X, Huang Y, Fang M, Wang J, Zheng Z, Su W (2005) Cytotoxic and antimicrobial metabolites from marine lignicolous fungi, Diaporthe sp. FEMS Microbiol Lett 251:53–58CrossRefGoogle Scholar
  21. Madiedo RP, de los Reyes-Gavilan CG (2005) Methods for the Screening, Isolation, and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria. J Dairy Sci 88:843–856CrossRefGoogle Scholar
  22. Maifreni M, Marino M, Conte L (2004) Lactic acid fermentation of Brassica rapa : chemical and microbial evaluation of a typical Italian product ( brovada ). Eur Food Res Technol 218:469–473CrossRefGoogle Scholar
  23. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG (2005) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 91:571–577CrossRefGoogle Scholar
  24. Mitsuda H, Yasumoto K, Iwami K (1966) Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyoto Shokuryo 19:210–214CrossRefGoogle Scholar
  25. Montet D, Ray RC, Zakhia-Rozis N (2014) Lactic acid fermentation of vegetables and fruits. Micro Trad Foods 108–140.  https://doi.org/10.13140/2.1.2374.1127
  26. Paramithiotis S, Hondrodimou OL, Drosinos EH (2010) Development of the microbial community during spontaneous cauliflower fermentation. Food Res Int 43:1098–1103CrossRefGoogle Scholar
  27. Paramithiotis S, Kouretas K, Drosinos EH (2014) Effect of ripening stage on the development of themicrobial community during spontaneous fermentation of green tomatoes. J Sci Food Agric 94:1600–1606CrossRefGoogle Scholar
  28. Periago MJ, Rincón F, Agüera MD, Ros G (2004) Mixture approach for optimizing lycopene extraction from tomato and tomato products. J Agric Food Chem 52(19):5796–5802CrossRefGoogle Scholar
  29. Piasecka-Jozwiak KJ, Rozmierska B, Chablowska KM, Stecka S, Skąpska M, Kliszcz E, Szkudzinska R (2013) Starter cultures for lactic acid fermentation of sweet pepper, pattypan squash and tomatoes. Pol J Food Nutr Sci 63:95–102Google Scholar
  30. Rhee SJ, Lee JE, Lee CH (2011) Importance of lactic acid bacteria in Asian fermented foods. Microbial Cell Factories 10(Suppl 1):S5.  https://doi.org/10.1186/1475-2859-10-S1-S5
  31. Sajur SA, Saguir FM, Manca de Nadra MC (2007) Effect of dominant specie of lactic acid bacteria from tomato on natural microflora development in tomato purée. Food Control 18:594–600CrossRefGoogle Scholar
  32. Samelis J, Maurogenakis F, Metaxopoulos J (1994) Characterisation of lactic acid bacteria isolated from naturally fermented Greek dry salami. Int J Food Microbiol 23:179–196CrossRefGoogle Scholar
  33. Silva-Filho A (2003) Genetic characterization of yeast population from fuel-alcohol distilleries to select dominating strains for heterologous gene expression, PhD thesis. 145Google Scholar
  34. Swan A (1954) The use of a bile-aesculin medium and of maxted's technique of lancefield grouping in the identification of enterococci (group d streptococci). J Clin Path 7:160–163CrossRefGoogle Scholar
  35. Tagg JR, McGivern AR (1971) Assay system for bacteriocins. Appl Microbiol 21(943):1971Google Scholar
  36. Tanasupawat S, Okada S, Komagata K (1998) Lactic acid bacteria found in fermented fish in Thailand. J Gen Appl Microbiol 44:193–200CrossRefGoogle Scholar
  37. Thomas TD (1973) Agar medium for differentiation of Streptococcus cremoris from other bacteria. NZJ Dairy Sci Techno1 8:70–71Google Scholar
  38. Wakil S, Dadou A (2011) Physiological properties of a microbial Community in Spontaneous Fermentation of maize (Zea mays) for Ogi production. Int Res J Microbiol 2(3):109–115Google Scholar
  39. Weiss A, Domig KJ, Kneifel W (2005) Comparison of selective Media for the Enumeration of probiotic enterococci from animal feed. Food Technol Biotechnol 43(2):147–155Google Scholar
  40. Wouters D, Grosu-Tudor S, Zamfir M, De Vuysta L (2012) Bacterial community dynamics, lactic acid bacteria species diversity and metabolite kinetics of traditional Romanian vegetable fermentations. J Sci Food Agric 93:749–760CrossRefGoogle Scholar
  41. Wouters D, Grosu-Tudor S, Zamfirb M, De Vuyst L (2013) Applicability of lactobacillus plantarum IMDO 788 as a starter culture to control vegetable fermentations. J Sci Food Agric 93:3352–3361CrossRefGoogle Scholar
  42. Zhishen J, Mengcheng T, fianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  1. 1.Laboratoire Microorganismes et Biomolecules Actives (LR03ES03), Faculté des Science de TunisUniversité de Tunis El ManarTunisTunisie

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