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Annals of Microbiology

, Volume 69, Issue 1, pp 17–27 | Cite as

Isolation of lactic acid bacteria from grape fruit: antifungal activities, probiotic properties, and in vitro detoxification of ochratoxin A

  • Bouzaiene TaroubEmail author
  • Lasram Salma
  • Ziadi Manel
  • Hadda-Imen Ouzari
  • Zohra Hamdi
  • Hamdi Moktar
Original Article
  • 211 Downloads

Abstract

The aim of this study was to isolate lactic acid bacteria from two varieties of grape bunch cultivated in Tunisia (cardinal and red-globe) in order to assess their ability to inhibit the growth of their most widespread contaminant, Aspergillus niger aggrégats and Aspergillus carbonarius. Antifungal activity of 18 isolates was investigated using overlay technique; selected isolates were than identified using 16s rDNA sequence analysis. Isolates with antifungal activities were screened out and studied for their probiotic properties using in vitro tests (tolerance to simulated gastric jus, bile salts, hydrophobicity properties).The most efficient strain was also investigated for its ability to reduce the concentration of ochratoxin A (OTA) on liquid medium. Determination of OTA content in media was released using HPLC analysis. Selected strains (RG7B) (C11C) and (RG8A) showing a good antifungal activities against Aspergillus niger aggrégats and Aspergillus carbonarius were identified as Pediococcus pentosaceus and Lactobacillus plantarum, respectively. Pediococcus pentosaceus (RG7B) showed promising potential probiotic characteristics and had a high ability for OTA removal after 48 h of incubation in both MRS and PBS media. The OTA removal percentage was significantly higher in MRS than in PBS media (84 and 25%, respectively). This study provides evidence for the control of black Aspergillus growth in grape and OTA detoxifying by the use of autochthones LAB strains having antifungal effect and probiotic potential.

Keywords

Lactic acid bacteria Antifungal activities Probiotic Ochratoxin a 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan 2018

Authors and Affiliations

  • Bouzaiene Taroub
    • 1
    Email author
  • Lasram Salma
    • 2
  • Ziadi Manel
    • 1
  • Hadda-Imen Ouzari
    • 3
  • Zohra Hamdi
    • 2
  • Hamdi Moktar
    • 1
  1. 1.Laboratory Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (INSAT)University of CarthageTunisTunisia
  2. 2.Laboratory of Molecular Physiology of Plants, Borj-Cedria Biotechnology CenterHammam-LifTunisia
  3. 3.Laboratory Microorganismes et Biomolécules Actives, Faculté des Sciences de TunisUniversity Tunis El ManarTunisTunisia

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