Advertisement

BioChip Journal

, Volume 12, Issue 4, pp 326–331 | Cite as

Development of Colorimetric Paper Sensor for Pesticide Detection Using Competitive-inhibiting Reaction

  • Hyeok Jung Kim
  • Yeji Kim
  • Su Jung Park
  • Chanho Kwon
  • Hyeran NohEmail author
Original Article
  • 51 Downloads

Abstract

Contamination by pesticides is an ever-increasing problem associated with fields of environmental management and healthcare. Accordingly, appropriate treatments are in demand. Pesticide detection methods have been researched extensively, aimed at making the detection convenient, fast, cost-effective, and easy to use. Among the various detecting strategies, paper-based assay is potent for real-time pesticide sensing due to its unique advantages including disposability, light weight, and low cost. In this study, a paper-based sensor for chlorpyrifos, an organophosphate pesticide, has been developed by layering three sheets of patterned plates. In colorimetric quantification of pesticides, the blue color produced by the interaction between acetylcholinesterase and indoxyl acetate is inhibited by the pesticide molecules present in the sample solutions. With the optimized paper-based sensor, the pesticide is sensitively detected (limit of detection=8.60 ppm) within 5 min. Furthermore, the shelf life of the device is enhanced to 14 days after from the fabrication, by treating trehalose solution onto the deposited reagents. We expect the paper-based device to be utilized as a first-screening analytic device for water quality monitoring and food analysis.

Keywords

Pesticide Paper microfluidic Colorimetric sensing Three-dimensional paper device 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Schreinemachers, P. & Tipraqsa, P. Agricultural pesticides and land use intensification in high, middle and low income countries. Food Policy 37, 616–626 (2012).CrossRefGoogle Scholar
  2. 2.
    Popp, J., Pető, K. & Nagy, J. Pesticide productivity and food security: a Review. Agron. Sustainable Dev. 33, 243–255 (2013).CrossRefGoogle Scholar
  3. 3.
    Jeyaratnam, J. Health problems of pesticide usage in the Third World. Br. J. Ind. Med. 42, 505–506 (1985).Google Scholar
  4. 4.
    Ecobichon, D.J. Pesticide use in developing countries. Toxicology 160, 27–33 (2001).CrossRefGoogle Scholar
  5. 5.
    Eddleston, M. et al. Pesticide poisoning in the developing world - a minimum pesticides list. The Lancet 360, 1163–1167 (2002).CrossRefGoogle Scholar
  6. 6.
    Martinez Andres, W., Phillips Scott, T., Butte Manish, J. & Whitesides George, M. Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew. Chem. Int. Ed. 46, 1318–1320 (2007).CrossRefGoogle Scholar
  7. 7.
    Chen, G.-H. et al. Detection of mercury (II) ions using colorimetric gold nanoparticles on paper-based analytical devices. Anal. Chem. 86, 6843–6849 (2014).CrossRefGoogle Scholar
  8. 8.
    Jokerst, J.C. et al. Development of a paper-based analytical device for colorimetric detection of select foodborne pathogens. Anal. Chem. 84, 2900–2907 (2012).CrossRefGoogle Scholar
  9. 9.
    Jayawardane, B.M., Wei, S., McKelvie, I.D. & Kolev, S.D. Microfluidic paper-based analytical device for the determination of nitrite and nitrate. Anal. Chem. 86, 7274–7279 (2014).CrossRefGoogle Scholar
  10. 10.
    Peters, K.L. et al. Simultaneous colorimetric detection of improvised explosive compounds using microfluidic paper-based analytical devices (μPADs). Anal. Methods 7, 63–70 (2015).CrossRefGoogle Scholar
  11. 11.
    Wu, Y., Sun, Y., Xiao, F., Wu, Z. & Yu, R. Sensitive inkjet printing paper-based colormetric strips for acetylcholinesterase inhibitors with indoxyl acetate substrate. Talanta 162, 174–179 (2017).CrossRefGoogle Scholar
  12. 12.
    Hossain, S.M.Z., Luckham, R.E., McFadden, M.J. & Brennan, J.D. Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. Anal. Chem. 81, 9055–9064 (2009).CrossRefGoogle Scholar
  13. 13.
    Hossain, S.M.Z. et al. Development of a bioactive paper sensor for detection of neurotoxins using piezoelectric inkjet printing of sol-gel-derived bioinks. Anal. Chem. 81, 5474–5483 (2009).CrossRefGoogle Scholar
  14. 14.
    Badawy, M.E. & El-Aswad, A.F. Bioactive paper sensor based on the acetylcholinesterase for the rapid detection of organophosphate and carbamate pesticides. Int. J. Anal. Chem. https://doi.org/10.1155/2014/536823 (2014).Google Scholar
  15. 15.
    Jahanshahi-Anbuhi, S. et al. Creating fast flow channels in paper fluidic devices to control timing of sequential reactions. Lab Chip 12, 5079–5085 (2012).CrossRefGoogle Scholar
  16. 16.
    Nouanthavong, S., Nacapricha, D., Henry, C.S. & Sameenoi, Y. Pesticide analysis using nanoceria-coated paper-based devices as a detection platform. Analyst 141, 1837–1846 (2016).CrossRefGoogle Scholar
  17. 17.
    Kavruk, M., Özalp, V.C. & Öktem, H.A. Portable bioactive paper-based sensor for quantification of pesticides. J. Anal. Methods Chem. https://doi.org/10.1155/2013/932946 (2013).Google Scholar
  18. 18.
    Chang, J., Li, H., Hou, T. & Li, F. Paper-based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity. Biosens. Bioelectron. 86, 971–977 (2016).CrossRefGoogle Scholar
  19. 19.
    Cotson, S. & Holt, S.J. IV. Kinetics of aerial oxidation of indoxyl and some of its halogen derivatives. Proc. R. Soc. Lond. B. Biol. Sci. 148, 506 (1958).CrossRefGoogle Scholar
  20. 20.
    Pohanka, M., Hrabinova, M., Kuca, K. & Simonato, J.-P. Assessment of acetylcholinesterase activity using indoxylacetate and comparison with the standard Ellman’s method. Int. J. Mol. Sci. 12, 2631–2640 (2011).CrossRefGoogle Scholar
  21. 21.
  22. 22.
    Han, Y. et al. Effects of sugar additives on protein stability of recombinant human serum albumin during lyophilization and storage. Arch. Pharmacal Res. 30, 1124 (2007).CrossRefGoogle Scholar

Copyright information

© The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of OptometrySeoul National University of Science and Technology (Seoultech)SeoulKorea
  2. 2.Research Institute, Biomax Co., Ltd., Seoul TechnoparkSeoulKorea
  3. 3.Convergence Institute of Biomaterials and BioengineeringSeoul National University of Science and Technology (Seoultech)SeoulKorea

Personalised recommendations