Contamination by pesticides is an ever-increasing problem associated with fields of environmental management and healthcare. Accordingly, appropriate treatments are in demand. Pesticide detection methods have been researched extensively, aimed at making the detection convenient, fast, cost-effective, and easy to use. Among the various detecting strategies, paper-based assay is potent for real-time pesticide sensing due to its unique advantages including disposability, light weight, and low cost. In this study, a paper-based sensor for chlorpyrifos, an organophosphate pesticide, has been developed by layering three sheets of patterned plates. In colorimetric quantification of pesticides, the blue color produced by the interaction between acetylcholinesterase and indoxyl acetate is inhibited by the pesticide molecules present in the sample solutions. With the optimized paper-based sensor, the pesticide is sensitively detected (limit of detection=8.60 ppm) within 5 min. Furthermore, the shelf life of the device is enhanced to 14 days after from the fabrication, by treating trehalose solution onto the deposited reagents. We expect the paper-based device to be utilized as a first-screening analytic device for water quality monitoring and food analysis.
Pesticide Paper microfluidic Colorimetric sensing Three-dimensional paper device
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Eddleston, M. et al. Pesticide poisoning in the developing world - a minimum pesticides list. The Lancet360, 1163–1167 (2002).CrossRefGoogle Scholar
Martinez Andres, W., Phillips Scott, T., Butte Manish, J. & Whitesides George, M. Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew. Chem. Int. Ed.46, 1318–1320 (2007).CrossRefGoogle Scholar
Chen, G.-H. et al. Detection of mercury (II) ions using colorimetric gold nanoparticles on paper-based analytical devices. Anal. Chem.86, 6843–6849 (2014).CrossRefGoogle Scholar
Jokerst, J.C. et al. Development of a paper-based analytical device for colorimetric detection of select foodborne pathogens. Anal. Chem.84, 2900–2907 (2012).CrossRefGoogle Scholar
Jayawardane, B.M., Wei, S., McKelvie, I.D. & Kolev, S.D. Microfluidic paper-based analytical device for the determination of nitrite and nitrate. Anal. Chem.86, 7274–7279 (2014).CrossRefGoogle Scholar
Peters, K.L. et al. Simultaneous colorimetric detection of improvised explosive compounds using microfluidic paper-based analytical devices (μPADs). Anal. Methods7, 63–70 (2015).CrossRefGoogle Scholar
Wu, Y., Sun, Y., Xiao, F., Wu, Z. & Yu, R. Sensitive inkjet printing paper-based colormetric strips for acetylcholinesterase inhibitors with indoxyl acetate substrate. Talanta162, 174–179 (2017).CrossRefGoogle Scholar
Hossain, S.M.Z., Luckham, R.E., McFadden, M.J. & Brennan, J.D. Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. Anal. Chem.81, 9055–9064 (2009).CrossRefGoogle Scholar
Hossain, S.M.Z. et al. Development of a bioactive paper sensor for detection of neurotoxins using piezoelectric inkjet printing of sol-gel-derived bioinks. Anal. Chem.81, 5474–5483 (2009).CrossRefGoogle Scholar
Chang, J., Li, H., Hou, T. & Li, F. Paper-based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity. Biosens. Bioelectron.86, 971–977 (2016).CrossRefGoogle Scholar
Cotson, S. & Holt, S.J. IV. Kinetics of aerial oxidation of indoxyl and some of its halogen derivatives. Proc. R. Soc. Lond. B. Biol. Sci.148, 506 (1958).CrossRefGoogle Scholar
Pohanka, M., Hrabinova, M., Kuca, K. & Simonato, J.-P. Assessment of acetylcholinesterase activity using indoxylacetate and comparison with the standard Ellman’s method. Int. J. Mol. Sci.12, 2631–2640 (2011).CrossRefGoogle Scholar