BioChip Journal

, Volume 12, Issue 4, pp 280–286 | Cite as

Fabrication of Multi-layered Macroscopic Hydrogel Scaffold Composed of Multiple Components by Precise Control of UV Energy

  • Donghyeon Roh
  • Woongsun Choi
  • Junbeom Kim
  • Hyun-Yong Yu
  • Nakwon ChoiEmail author
  • Il-Joo ChoEmail author
Original Article


Hydrogel scaffolds composed of multiple components are promising platform in tissue engineering as a transplantation materials or artificial organs. Here, we present a new fabrication method for implementing multi-layered macroscopic hydrogel scaffold composed of multiple components by controlling height of hydrogel layer through precise control of ultraviolet (UV) energy density. Through the repetition of the photolithography process with energy control, we can form several layers of hydrogel with different height. We characterized UV energy-dependent profiles with single-layered PEGDA posts photocrosslinked by the modular methodology and examined the optical effect on the fabrication of multi-layered, macroscopic hydrogel structure. Finally, we successfully demonstrated the potential applicability of our approach by fabricating various macroscopic hydrogel constructs composed of multiple hydrogel layers.


Photocrosslinking Macroscopic hydrogel scaffold Height control via UV energy density Multilayered structures Incorporation of multiple components Tissue engineering Artificial tissues/organs 


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Copyright information

© The Korean BioChip Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Center for BioMicrosystems, Brain Science InstituteKorea Institute of Science and Technology (KIST)SeoulRepublic of Korea
  2. 2.School of Electrical EngineeringKorea UniversitySeoulRepublic of Korea
  3. 3.Department of Chemical and Biological EngineeringKorea UniversitySeoulRepublic of Korea
  4. 4.Division of Bio-Medical Science & Technology, KIST SchoolKorea University of Science and Technology (UST)SeoulRepublic of Korea

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