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Draft genome sequence of Actinocorallia populi A251T, an actinomycetes producing polyketides and nonribosomal polypeptides


In this work, we present the draft genome sequence of Actinocorallia populi A251T consisting of 8,253,402 bp with a G + C content of 71.5 mol%. The genome sequence includes 3 5S rRNA genes, 54 tRNA genes, 28 snRNA genes and 19 CRISPRs in 26 contigs. Using the Rapid Annotation using Subsystem Technology, a total of 7766 coding DNA sequences, which were assigned to 436 subsystems, were found in the genome. And 6743 protein-coding sequences with designated functions were assigned to 19 categories in the Cluster of Orthologous Groups database. The genome contains 20 gene clusters responsible for the synthesis of secondary metabolites, including two type I polyketide synthase (PKS) gene clusters, two type III PKS gene clusters, three nonribosomal peptide synthetase (NRPS) gene clusters and two hybrid PKS/NRPS gene clusters. These results indicate that strain A251 has the ability to produce several nonribosomal polypeptides and diverse polyketides, which was verified by liquid chromatography-mass spectrometry analysis of the extraction from fermentation broth. In addition, lactones, indole and many unknown natural products are present in the metabolites. Bioactivity testing revealed anticancer and antidiabetic activities of the crude extract towards the cancer cell line HepG2. In summary, the genomic sequences and bioactive fermentation products demonstrate the potential of strain A251 in biotechnology.

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Fig. 1



Coding DNA sequences


Polyketide synthase




Nonribosomal polypeptide synthetase


Nonribosomal polypeptide


Biosynthetic gene cluster


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We thank the Institute of Materia Medica of Chengdu Diao pharmaceutical Corp. for conducing the anticancer and antidiabetic activity screening. This work was supported by the National Key Research and Development Program of China (2017YFB0308401); and the Opening Project of Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education (20826041C4159).

Author information

All the authors contributed to this work. YT supported and guided the experiments; XL performed the experiments and analyzed the data, and wrote the paper; ZW and LG participated in the data analysis. All authors reviewed and approved the manuscript.

Correspondence to Yongqiang Tian.

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This work does not contain any studies with human participants or animals performed by any of the authors.

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Li, X., Wang, Z., Gan, L. et al. Draft genome sequence of Actinocorallia populi A251T, an actinomycetes producing polyketides and nonribosomal polypeptides. 3 Biotech 10, 79 (2020).

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  • Draft genome
  • Actinocorallia populi
  • Genomic annotation
  • Crude extract