Questin has favorable applications. Fractional factorial design, Box–Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, Aspergillus flavipes HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical flask with 200 mL of medium were 4% soluble starch, 0.9% beef extract, 4% NaCl, 0.05% Na2HPO4, pH 6, 2% inoculum size, and shaking at 28 ℃ and 160 rpm/min for 7 days. The production of questin can achieve 64.93 ± 4.55 mg/L, with no significant difference from the predicted value (66.27 mg/L). Thus, this optimized process of questin production is feasible. Such production is 17-fold higher than that of the basal Sabouraud’s dextrose medium. Results indicate the potential of A. flavipes HN4-13 in the large-scale production of questin through fermentation.
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This work financially supported by the Open-end Funds of Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening (HY201803), Natural Science Foundation of Jiangsu Higher Education Department (19KJB350007), Natural Science Foundation of Jiangsu Province (BK20151283, BK20181484), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Postgraduate Research and Practice Innovation Program of Jiangsu Province (SJCX19_1001).
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The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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Guo, L., Wang, L., Li, X. et al. Enhanced production of questin by marine-derived Aspergillus flavipes HN4-13. 3 Biotech 10, 54 (2020). https://doi.org/10.1007/s13205-020-2067-z
- Aspergillus flavipes HN4-13
- Fermentation optimization
- Response surface methodology