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3 Biotech

, 10:36 | Cite as

Zinc solubilizing bacteria (Bacillus megaterium) with multifarious plant growth promoting activities alleviates growth in Capsicum annuum L.

  • Kalpana BhattEmail author
  • Dinesh Kumar Maheshwari
Original Article
  • 52 Downloads

Abstract

The present study was designed to isolate an array of zinc solubilizing bacteria (ZSB) and to characterize them for plant growth promotion (PGP) attributes with respect to Capsicum annuum L. For this purpose, seventy bacteria were procured from cow dung and screened for zinc solubilization (ZnO and ZnCO3). Where, isolate CDK25 was found to be the most potent owing to its maximum zinc solubilization (ZnO) ability (5.0 cm). For quantitative assay, atomic absorption spectroscopy (AAS) was used, where CDK25 showed markedly higher solubilization of ZnO (20.33 ppm). It was investigated that CDK25 also endowed with multiple PGP attributes viz., Phosphate solubilization, Phytase production, Indole acetic acid (IAA) and Siderophore production. Quantitative study revealed isolate CDK25 to solubilize and produce maximum quantity of phosphate (281.59 μg/ml) and IAA (13.8 μg/ml) respectively. ZSB was applied in different treatments under pot culture assay, where T3 (seeds + CDK25) showed significant impact on plant growth parameters, besides showing maximum zinc content in fruit (0.25 mg/100 g). Hence, isolate CDK25 expresses highest potential throughout the experiments; as zinc solubilizer, PGP strain, and based on 16S rRNA gene sequencing identified as Bacillus megaterium. Therefore, meticulous use of this bacterium could aid in providing adequate amount of soluble zinc along with enhanced plants growth, nutrient uptake and yield in sustainable manner.

Keywords

Zinc solubilizing bacteria (ZSB) Cow dung Capsicum annuumBacillus megaterium PGP 

Notes

Acknowledgements

The authors thank Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar, Uttrakhand, India, for providing necessary facilities to carry out this research.

Author contributions

KB assisted during the experiments and prepared the manuscript. DKM corrected and finalized the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

References

  1. Abaid-Ullah M, Nadeem M, Hassan M, Ganter J, Muhammad B, Nawaz K, Shah AS, Hafeez FY (2015) Plant growth promoting rhizobacteria: an alternate way to improve yield and quality of wheat (Triticum aestivum). Int J Agric Biol 17(1):51–60Google Scholar
  2. Ahmad F, Ahmad I, Khan MS (2008) Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 163:173–181PubMedGoogle Scholar
  3. Alloway BJ (2008) Zinc in soils and crop nutrition. 2nd edn. IZA/IFA, Brussels, Belgium/Paris, FranceGoogle Scholar
  4. Bakhshandeh E, Pirdashti H, Shahsavarpour Lendeh K (2017) Phosphate and potassium-solubilizing bacteria effect on the growth of rice. Ecol Eng 103:164–169Google Scholar
  5. Barzanti R, Ozino F, Bazzicalupo M, Gabbrielli R, Galardi F, Gonnelli C, Mengoni A (2007) Isolation and characterization of endophytic bacteria from the nickel hyperaccumulator plant Alyssum bertolonii. Microb Ecol 53:306–316PubMedGoogle Scholar
  6. Bhatt K, Maheshwari DK (2019) Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L. Sci Rep 9(1):1–10Google Scholar
  7. Budaraga IK (2015) Cattle cow dung use as an alternative energy source and organic fertilizer friendly enviroment village Kasang districts Batang Anai Padang Pariaman. Int J Sci Technol Res 4(8):171–175Google Scholar
  8. Chauhan AK, Maheshwari DK, Kim K, Bajpai VK (2016) Termitarium inhabiting Bacillus endophyticus TSH42 and Bacillus cereus TSH77 colonizing Curcuma longa L.: isolation, characterization and evaluation of their biocontrol and plant growth promoting activities. Can J Microbiol 62:880–892PubMedGoogle Scholar
  9. Davies PJ (2010) The plant hormones: their nature, occurrence, and functions. Plant Hormones. Springer, Netherlands, pp 1–15Google Scholar
  10. Dell'Amico E, Cavalca L, Andreoni V (2008) Improvement of Brassica napus growth under cadmium stress by cadmium-resistant rhizobacteria. Soil Biol Biochem 40:74–84Google Scholar
  11. Desai S, Kumar P, Sultana U, Pinisetty S, Reddy G (2012) Potential microbial candidate strains for management of nutrient requirements of crops. Afr J Microbiol Res 6(17):3924–3931Google Scholar
  12. Devi NC, Mazumder PB, Bhattacharjee A (2018) Statistical optimization of polyhydroxybutyrate production by Bacillus Pumilus H9 using cow dung as a cheap carbon source by response surface methodology. J Polym Environ 26(8):3159–3167Google Scholar
  13. Di Simine CD, Sayer JA, Gadd GM (1998) Solubilization of zinc phosphate by a strain of Pseudomonas fluorescens isolated from a forest soil. Biol Fertil Soils 28(1):87–94Google Scholar
  14. Dinesha R, Srinivasana V, Hamza S, Sarathambal C, Ankegowda SJ, Ganeshamurthy AN, Divya VC (2018) Isolation and characterization of potential Zn solubilizing bacteria from soil and its effects on soil Zn release rates, soil available Zn and plant Zn content. Geoderma 321:173–186Google Scholar
  15. Dubey RC, Maheshwari DK (2012) Practical microbiology. S. Chand Pvt Limited, ChennaiGoogle Scholar
  16. Dubey RC, Khare S, Kumar P, Maheshwari DK (2014) Combined effects of chemical fertilizers and rhizosphere-competent Bacillus subtilis BSK17 on yield of Cicer arietinum. Arch Phytopathol Plant Protect 47:2305–2318Google Scholar
  17. Fasim F, Ahmed N, Parsons R, Gadd GM (2002) Solubilization of zinc salts by a bacterium isolated from the air environment of a tannery. FEMS Microbiol Lett 213(1):1–6PubMedGoogle Scholar
  18. Fronning BE, Thelen KD, Min DH (2008) Use of manure compost, and cover crops to supplant crop residue carbon in corn stover removed cropping systems. Agronomy J 100:1703–1710Google Scholar
  19. Gandhi A, Muralidharan G, Sudhakar E, Murugan A (2014) Screening for elite zinc solubilizing bacterial isolate from rice rhizosphere environment. Int J Recent Sci Res 5:2201–2204Google Scholar
  20. Golabi MH, Denney MJ, Iyekar C (2004) Use of composted organic waste as alternative to synthetic fertilizers for enhancing crop productivity and agricultural sustainability on the tropical island of Guam. Proceeding of 13th International Soil Conservation Organization Conferences Brisbane 234: 1–6.Google Scholar
  21. Gudugi IAS (2013) Effects of cow dung and variety on the growth and yield of Okra (Abelmuschus esculentus L). Eur J Exp Biol 3(2):495–498Google Scholar
  22. Hafeez B, Khanif YM, Saleem M (2013) Role of zinc in plant nutrition—a review. Am J Exp Agric 3:374–391Google Scholar
  23. Hameeda B, Rupela OP, Reddy G, Satyavani K (2006) Application of plant growth-promoting bacteria associated with composts and macrofauna for growth promotion of Pearl millet (Pennisetum glaucum L). Biol Fertil Soils 43(2):221–227Google Scholar
  24. Holt JG, Kreig NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, BaltimoreGoogle Scholar
  25. Hue NV, Silva JA (2000) Organic soil amendements for sustainable agriculture: organic sources of nitrogen, phosphorus, and potassium. In: Silva JA and Uchida R (eds) College of tropical agriculture and human resources. University of Hawaii, Manoa pp 133–143.Google Scholar
  26. Idris R, Trifonova R, Puschenreiter M, Wenzel WW, Sessitsch A (2004) Bacterial communities associated with flowering plants of the Ni hyperaccumulator Thlaspi goesingense. Appl Environ Microbiol 70:2667–2677PubMedPubMedCentralGoogle Scholar
  27. Jepkoech JK, Simiyu GM, Arusei M (2013) Selected heavy metals in water and sediments and their bioconcentrations in plant (Polygonum pulchrum) in Sosiani River. Uasin Gishu County Kenya J Environ Protect 4:796–802Google Scholar
  28. Kang SM, Radhakrishnan R, You YH, Joo GJ, Lee IJ, Lee KE, Kim JH (2014) Phosphate solubilizing Bacillus megaterium mj1212 regulates endogenous plant carbohydrates and amino acids contents to promote mustard plant growth. Indian J Microbiol 54(4):427–433PubMedPubMedCentralGoogle Scholar
  29. Kloepper JW, Ryum M, Zhang S (2004) Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathol 94:1259–1266Google Scholar
  30. Ma Y, Rajkumar M, Freitas H (2009) Isolation and characterization of Ni mobilizing PGPB from serpentine soils and their potential in promoting plant growth and Ni accumulation by Brassica spp. Chemosphere 75:719–725PubMedGoogle Scholar
  31. Masciarelli O, Urbani L, Reinoso H, Luna V (2013) Alternative mechanism for the evaluation of indole-3-acetic acid (IAA) production by Azospirillum brasilense strains and its effects on the germination and growth of maize seedlings. J Microbiol 51:590–597PubMedGoogle Scholar
  32. Mishra IG, Sapre S, Tiwari S (2017) Zinc solubilizing bacteria from the rhizosphere of rice as prospective modulator of zinc biofortification in rice. Rhizosphere 3(1):185–190Google Scholar
  33. Nahas E (1996) Factors determining rock phosphate solubilization by microorganisms isolated from soil. World J Microbiol Biotechnol 12:567–572PubMedGoogle Scholar
  34. Palaniappan P, Chauhan PS, Saravanan VS, Anandham R, Sa T (2010) Isolation and characterization of plant growth promoting endophytic bacterial isolates from root nodule of Lespedeza sp. Biol Fertil Soils 46(8):807–816Google Scholar
  35. Pandey C, Bajpai VK, Negi YK, Rather IA, Maheshwari DK (2018) Effect of plant growth promoting Bacillus spp on nutritional properties of Amaranthus hypochondriacus grains. Saudi J Biol Sci 25(6):1066–1071PubMedPubMedCentralGoogle Scholar
  36. Pawar A, Ismail S, Mundhe S, Patil VD (2015) Solubilization of insoluble zinc compounds by different microbial isolates in vitro condition. Int J Trop Agric 33:865–869Google Scholar
  37. Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17:362–370Google Scholar
  38. Rajkumar M, Freitas H (2008) Effects of inoculation of plant growth promoting bacteria on Ni uptake by Indian mustard. Bioresour Technol 99:3491–3498PubMedGoogle Scholar
  39. Ramesh A, Sharma SK, Sharma MP, Yadav N, Joshi OP (2014) Inoculation of zinc solubilizing Bacillus aryabhattai strains for improved growth, mobilization and biofortification of zinc in soybean and wheat cultivated in Vertisols of central India. Appl Soil Ecol 73:87–96Google Scholar
  40. Russell FM, Biribo SSN, Selvaraj G, Oppedisano F, Warren S, Seduadua A, Mulholland EK, Carapetis JR (2006) As a bacterial culture medium, citrated sheep blood agar is a practical alternative to citrated human blood agar in laboratories of developing countries. J Clin Microbiol 44(9):3346–3351PubMedPubMedCentralGoogle Scholar
  41. Sadiq HM, Jahangir GZ, Nasir IA, Iqtidar M, Iqbal M (2014) Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soil. Biotech Biotech Equip 27:4248–4255Google Scholar
  42. Sambrook J, Russel D (2001) Molecular cloning A Laboratory Manual Third ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY 17–18Google Scholar
  43. Sayer JA, Gadd GM (1997) Solubilization and transformation of insoluble metal compounds to insoluble metal oxalates by Aspergillus niger”. Mycol Res 101:651–653Google Scholar
  44. Schwyn B, Neilands JB (1987) Universal chemical assay for detection and determination of siderophores. Ann Biochem 160:47–56Google Scholar
  45. Shao J, Xu Z, Zhang N, Shen Q, Zhang R (2015) Contribution of indole-3-acetic acid in the plant growth promotion by the rhizospheric strain Bacillus amyloliquefaciens SQR9. Biol Fertil Soils 51(3):321–330Google Scholar
  46. Sharma P, Kunawat KC, Kaur S, Kaur N (2014) Assessment of zinc solubilization by endophytic bacteria in legume rhizosphere. Ind J Appl Res 4:439–441Google Scholar
  47. Sunithakumari K, Padma Devi SN, Vasandha S (2016) Zinc solubilizing bacterial isolates from the agricultural fields of Coimbatore, Tamil Nadu India. Curr Sci (00113891)110(2)Google Scholar
  48. Swain MR, Ray RC (2009) Biocontrol and other beneficial activities of Bacillus subtilis isolated from cowdung microflora. Microbiol Res 164:121–130PubMedGoogle Scholar
  49. Talboys PJ, Owen DW, Healey JR, Withers PJA, Jones DL (2014) Auxin secretion by Bacillus amyloliquifaciens FZB42 both stimulates root exudation and limits phosphorus uptake in Triticum aestivium. BMC Plant Biol 14:51PubMedPubMedCentralGoogle Scholar
  50. Tavallali V, Rahemi M, Eshghi S, Kholdebarin B, Ramezanian A (2010) Zinc alleviates salt stress and increases antioxidant enzyme activity in the leaves of pistachio (Pistacia vera L'.Badami') seedlings. Turk J Agric For 34:349–359Google Scholar
  51. Vidyashree ND (2016) Isolation and characterization of Zinc solubilizing bacteria from stone quarry dust powder. Intl J Agri Sci ISSN 0975–3710Google Scholar
  52. Weller DM, Cook RJ (1983) Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads. Phytopathology 73(3):463–469Google Scholar
  53. Yu X, Liu X, Zhu TH, Liu GH, Mao C (2011) Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization. Biol Fertil Soils 47(4):437–446Google Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2020

Authors and Affiliations

  1. 1.Department of Botany and MicrobiologyGurukul Kangri VishwavidyalayaHaridwarIndia

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