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3 Biotech

, 10:50 | Cite as

Synergistic effect of ascorbic acid and taurine in the treatment of a spinal cord injury-induced model in rats

  • Chao ChenEmail author
  • Qiang Yang
  • Xinlong Ma
Original Article
  • 15 Downloads

Abstract

Spinal cord injury (SCI) results in severe damage, which causes functional alterations together with loss of autonomic functions, sensations, and muscle functioning. This injury leads to apoptosis of neurons and oligodendrocytes, which further leads to dysfunction of the spinal cord due to axonal degeneration and demyelination. Taurine is non-proteogenic and an essential amino acid, which plays a major role in the growth and development of brain cells. Ascorbic acid, also known as vitamin C, is found in various foods and is known to prevent scurvy. In this study, we have investigated the therapeutic effect of ascorbic acid and taurine against SCI-induced rats. The rats were divided into the following groups: sham, control, 100 mg/kg of taurine, 100 mg/kg of ascorbic acid, and 100 mg/kg of taurine + 100 mg/kg of ascorbic acid. Treatment was continued daily for 45 consecutive days. The combined treatment of taurine and ascorbic acid decreased caspase-3, bax, pro-NGF, and p53 mRNA expression by more than 30% compared to individual treatments. The combined treatment of taurine and ascorbic acid reduced caspase-3 and p53 expression by 33.7% and 44%, respectively, compared to individual treatments. The combined treatment of taurine and ascorbic acid decreased mRNA expression of interleukin-6 (IL-6), cyclooxygenase-2, tumor necrosis factor-alpha (TNF-α), and inducible nitric oxide synthase (iNOS) compared to the individual treatments of taurine and ascorbic acid. The combined treatment of taurine and ascorbic acid also significantly recovered altered antioxidant markers, and induced lipid peroxidation to near normal levels. In summary, apoptotic, inflammatory and oxidative stress markers were significantly decreased in SCI-induced rats treated with taurine and ascorbic acid.

Keywords

Taurine Ascorbic acid Spinal cord injury Apoptosis Inflammation 

Notes

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

References

  1. Afshari JT, Ghomian N, Shameli A, Shakeri MT, Fahmidehkar MA, Mahajer E, Khoshnavaz R, Emadzadeh M (2005) Determination of interleukin-6 and tumor necrosis factor-alpha concentrations in Iranian-Khorasanian patients with preeclampsia. BMC Pregnancy Childbirth 5:14CrossRefGoogle Scholar
  2. Ali AA, Abd Al Haleem EN, Khaleel SA, Sallam AS (2017) Protective effect of cardamonin against acetic acid-induced ulcerative colitis in rats. Pharmacol Rep 69(2):268–275CrossRefGoogle Scholar
  3. Arutyunyan TV, Korystova AF, Kublik LN, Levitman MKh, Shaposhnikova VV, Korystov YN (2016) Taxifolin and fucoidin abolish the irradiation-induced increase in the production of reactive oxygen species in rat aorta. Bull Exp Biol Med 160:635–638CrossRefGoogle Scholar
  4. Bao F, Liu D (2002) Peroxynitrite generated in the rat spinal cord induces neuron death and neurological deficits. Neuroscience 115:839–849CrossRefGoogle Scholar
  5. Bernal F, Hartung HP, Kieseier BC (2005) Tissue mRNA expression in rat of newly described matrix metalloproteinases. Biol Res 38(2–3):267–271PubMedGoogle Scholar
  6. Hussein J, El-Banna M, Razik TA, El-Naggar ME (2018) Biocompatible zinc oxide nanocrystals stabilized via hydroxyethyl cellulose for mitigation of diabetic complications. Int J Biol Macromol 107(Pt A):748–754CrossRefGoogle Scholar
  7. Kaddour T, Omar K, Oussama AT, Nouria H, Iméne B, Abdelkader A (2016) Aluminium-induced acute neurotoxicity in rats: treatment with aqueous extract of Arthrophytum (Hammada scoparia). J Acute Dis 5(6):470–482CrossRefGoogle Scholar
  8. Katoh D, Ikata T, Katoh S, Hamada Y, Fukuzawa K (1996) Effect of dietary vitamin C on compression injury of the spinal cord in a rat mutant unable to synthesize ascorbic acid and its correlation with that of vitamin E. Spinal Cord 34(4):234–238CrossRefGoogle Scholar
  9. Krishna V, Andrews H, Jin X, Yu J, Varma A, Wen X, Kindy M (2013) A contusion model of severe spinal cord injury in rats. J Vis Exp 78:50111Google Scholar
  10. Leon R, Wu H, Jin Y, Wei J, Buddhala C, Prentice H, Wu JY (2009) Protective function of taurine in glutamate-induced apoptosis in cultured neurons. J Neurosci Res 87(5):1185–94CrossRefGoogle Scholar
  11. Liu J, Head E, Gharib AM, Yuan W, Ingersoll RT, Hagen TM, Cotman CW, Ames BN (2002) Memory loss in old rats is associated with brain mitochondrial decay and RNA/DNA oxidation: partial reversal by feeding acetyl-l-carnitine and/or R-α-lipoic acid. Proc Natl Acad Sci USA 99:2356–2361CrossRefGoogle Scholar
  12. Min KJ, Pyo HK, Yang MS, Ji KA, Jou I, Joe EH (2004) Gangliosides activate microglia via protein kinase C and NADPH oxidase. Glia 48:197–206CrossRefGoogle Scholar
  13. Minakov AN, Chernov AS, Asutin DS, Konovalov NA, Telegin GB (2018) Experimental models of spinal cord injury in laboratory rats. Acta Naturae 10(3):4–10CrossRefGoogle Scholar
  14. Nakajima Y, Osuka K, Seki Y, Gupta RC, Hara M, Takayasu M, Wakabayashi T (2010) Taurine reduces inflammatory responses after spinal cord injury. J Neurotrauma 27(2):403–410CrossRefGoogle Scholar
  15. Olive MF (2002) Interactions between taurine and ethanol in the central nervous system. Amino Acids 23(4):345–57CrossRefGoogle Scholar
  16. Schuller-Levis GB, Park E (2003) Taurine: new implications for an old amino acid. FEMS Microbiol Lett 226:195–202CrossRefGoogle Scholar
  17. Shaheen TI, El-Naggar MI, Hussein JS, El-Bana M, Emara E, El-Khayat Z, Fouda MMG, Ebaid H, Hebeish A (2016) Antidiabetic assessment; in vivo study of gold and core-shell silver-gold nanoparticles on streptozotocin-induced diabetic rats. Biomed Pharmacother 83:865–875CrossRefGoogle Scholar
  18. Tsuboyama-Kasaoka N, Shozawa C, Sano K, Kamei Y, Kasaoka S, Hosokawa Y, Ezaki O (2006) Taurine (2-aminoethanesulfonic acid) deficiency creates a vicious circle promoting obesity. Endocrinology 147(7):3276–84CrossRefGoogle Scholar
  19. Wang WG, Xiu RJ, Xu ZW, Yin YX, Feng Y, Cao XC, Wang PS (2015) Protective effects of vitamin C against spinal cord injury-induced renal damage through suppression of NF-κB and proinflammatory cytokines. Neurol Sci 36(4):521–526CrossRefGoogle Scholar
  20. Wang Q, Fan W, Cai Y, Wu Q, Mo L, Huang Z, Huang H (2016) Protective effects of taurine in traumatic brain injury via mitochondria and cerebral blood flow. Amino Acids 48(9):2169–2177CrossRefGoogle Scholar
  21. Yan M, Yang M, Shao W, Mao XG, Yuan B, Chen YF, Ye ZX, Liang W, Luo ZJ (2014) High-dose ascorbic acid administration improves functional recovery in rats with spinal cord contusion injury. Spinal Cord 52(11):803–808CrossRefGoogle Scholar
  22. Yanagita T, Han SY, Hu Y, Nagao K, Kitajima H, Murakami S (2008) Taurine reduces the secretion of apolipoprotein B100 and lipids in HepG2 cells. Lipids Health Dis 7:38CrossRefGoogle Scholar
  23. Yu Q, Li X, Cao X (2016) Cardioprotective effects of phenylethanoid glycoside-rich extract from Cistanche deserticola in ischemia–reperfusion-induced myocardial infarction in rats. Ann Vasc Surg 34:234–242CrossRefGoogle Scholar
  24. Zhang M, Izumi I, Kagamimori S, Sokejima S, Yamagami T, Liu Z, Qi B (2004) Role of taurine supplementation to prevent exercise-induced oxidative stress in healthy young men. Amino Acids 26(2):203–207CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2020

Authors and Affiliations

  1. 1.Department of Spine SurgeryTianjin HospitalTianjinChina

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