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Validation of mouse phosphoprotein enriched in astrocyte 15 (mPEA15) expressing transgenic pig as a potential model in diabetes translational research

Abstract

The present study aimed to investigate the characteristics of mPEA15 expressing transgenic pig (TG pig) as a potential model for diabetes. Expression analysis confirmed the ubiquitous expression of mPEA15 in TG pigs at F4. Oral glucose tolerance test results showed that restoration of normal glucose levels was significantly delayed in the TG pigs when compared with that in the wild-type pigs (WT pigs). Primary skeletal muscle cells isolated from TG pigs demonstrated reduced glucose uptake and reduced GLUT4 translocation to the plasma membrane in response to insulin treatment. Combined, these results suggest that mPEA15 expressing pigs has a glucose intolerance and insulin resistance which are known to mediate the pathophysiology of type 2 diabetes mellitus. Thus, mPEA15 transgenic pigs would serve as a promising model for diabetes translational research.

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Acknowledgements

This study was supported by the 2009–2011 RDA Fellowship Program of the National Institute of Animal Science, Rural Development Administration, Republic of Korea. This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ007181)” Rural Development Administration, Republic of Korea.

Author information

Experimental design/concept: HL, JW and KK; Experiments handling: HK, KBO, SB, SL, SH, and BY; data analysis/interpretation: HL, HK and BMKV; drafting article: BMKV and HL; Critical revision of article: HL and HC.

Correspondence to Hwi-Cheul Lee.

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I testify on behalf of all co-authors that: this article has not been submitted in whole or in part elsewhere. This article is not under consideration for publication in any other journal. All the authors read and approve the final content of the manuscript.

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Vasamsetti, B.M.K., Kim, H., Kim, K. et al. Validation of mouse phosphoprotein enriched in astrocyte 15 (mPEA15) expressing transgenic pig as a potential model in diabetes translational research. 3 Biotech 10, 34 (2020). https://doi.org/10.1007/s13205-019-2021-0

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Keywords

  • Diabetes
  • Glucose tolerance
  • GLUT4
  • Insulin resistance
  • PEA15
  • PED
  • Transgenic pig