Functional studies have revealed miRNAs play pivotal roles in ovulation and ovary development in mammalians, whereas little is known about the miRNA function in ducks. In this study, miRNA deep sequencing in the ovary tissues was carried out to obtain the miRNA profile from ovaries before oviposition (BO) and after oviposition (AO) in Jinding duck. Overall, an average of 23,128,075 and 26,020,523 reads were identified in the BO and AO samples, respectively, and 6739 miRNAs were identified from them through further mapping and analysis. Besides, 1570 miRNAs were identified as differentially expressed miRNAs compared with BO, including 493 miRNAs up-regulated and 1077 down-regulated in AO. Moreover, 2291 target genes were predicted from 443 significantly differentially expressed miRNAs. In addition, GO and KEGG pathway analysis indicated that target genes were enriched in some basic cell metabolism pathways as well as the productive pathways such as MAPK signaling pathway, gonadotropin-releasing hormone signaling pathway, TGF-beta signaling pathway which had been significantly changed. Our results helped to replenish the duck miRNA database and illustrate the potential mechanism of miRNA function in duck ovary development and reproduction process.
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This work was supported by the National key research and development program (No. 2016YFD0500510).
The animal experiment in this study was approved via the animal care and ethical committee of Sichuan Animal Science Academy. All ducks were carried out on the guidelines of China legislations on the ethical use and care of laboratory animals.
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Supplemental Fig. 2. The number of reads mapped to mallard, chicken and zebra finch and identified as potential novel miRNAs. AO-1 to AO-3 samples represents 3 biological replicates of Jinding duck after oviposition. BO-1 to BO-3 samples represents 3 biological replicates before oviposition. (JPEG 177 kb)
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Yang, C., Xiong, X., Jiang, X. et al. Novel miRNA identification and comparative profiling of miRNA regulations revealed important pathways in Jinding duck ovaries by small RNA sequencing. 3 Biotech 10, 38 (2020). https://doi.org/10.1007/s13205-019-2015-y
- Duck ovaries
- Small RNA sequencing