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3 Biotech

, 9:421 | Cite as

Zinc biosorption, biochemical and molecular characterization of plant growth-promoting zinc-tolerant bacteria

  • Ramandeep Kour
  • Devendra JainEmail author
  • Ali Asger Bhojiya
  • Aradhana Sukhwal
  • Suman Sanadhya
  • Heena Saheewala
  • Gajanand Jat
  • Abhijeet Singh
  • Santosh Ranjan Mohanty
Original Article

Abstract

Zinc plays a key role in plant nutrition at low levels; however, at higher concentrations Zn ions can be highly phytotoxic and plant growth-promoting rhizobacteria can be used to reduce such metal toxicity. In the present investigation we had reported the zinc biosorption and molecular characterization of plant growth-promoting zinc-tolerant bacteria. Initially, thirty bacteria having zinc solubilizing ability were screened for MIC against zinc ion and displayed high value of MIC ranging from 2.5 to 62.5 mM. Biochemically, all the 30 isolates showed significant difference in the 6 biochemical tests performed. The molecular diversity studies based on the repetitive DNA PCR viz, REP, ERIC and BOX elements showed significant genetic diversity among these 30 zinc-tolerant bacteria. These ZTB strains also showed multiple PGP activities and all ZTB strains were found positive for production of IAA, GA3 and ammonia, whereas 24 were found positive for ACC deaminase activity, 8 showed siderophore production and 9 ZTB isolates were positive for HCN production. Out of 30 isolates, 24 showed phosphorus solubilization activity, 30 showed potash solubilization, 15 showed silica solubilization and 27 showed phytase production activities. All the 30 ZTB stains showed zinc solubilization up to 0.25% insoluble ZnO in the medium, whereas at 2% ZnO in MSM only 12 isolates showed solubilization which were further selected for zinc biosorption and pot studies. The heavy metal removal studies revealed that ZTB stains were able to remove zinc ions effectively from the medium efficiently and the highest zinc biosorption (< 90%) was recorded with the bacterial strain Z-15. Further, the inoculation of ZTB strains under zinc stress conditions (pot containing 1000 mg/kg Zn) resulted in significant increase of shoot length, root length and total chlorophyll content in maize seedlings compared with the uninoculated control. The partial 16S rDNA sequence of the potential ZTB isolates viz. Z-15, Z-24, Z-28 and Z-29 revealed their identity as Serratia sp. The ability of these zinc-tolerant bacteria to tolerate the toxic level of zinc may serve as suitable candidates for developing microbial formulations for the growth of crop plants in Zn-contaminated areas.

Keywords

Zinc-tolerant bacteria Zinc solubilization Biosorption Molecular characterization PGPR 

Abbreviations

ZTB

Zinc-tolerant bacteria

Zn

Zinc

MIC

Minimum inhibitory concentration

AAS

Atomic Absorption Spectroscopy

ERIC

Enterobacterial repetitive intergenic consensus

REP

Repetitive extragenic palindromic

BOX

BOX-A1R-based repetitive extragenic palindromic

UPGMA

Unweighted pair group method with arithmetic mean

PGPR

Plant growth-promoting rhizobacteria

Notes

Acknowledgements

The financial assistance from All India Network Project on soil biodiversity and biofertilizers and Rastriya Krishi Vikas Yojana (RKVY) are highly acknowledged. Ms. Suman Sanadhaya thanks CSIR for the award of Senior Research Fellowships. The support from Dean, RCA and Director of Research, MPUAT is also greatfully acknowledged.

Author contributions

DJ and SRM designed the research. RK, AAB, AS, SS, HS performed the experiments and interpreted the data. GJ performed soil and AAS analysis. DJ, SRM and AS wrote the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13205_2019_1959_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Ramandeep Kour
    • 1
  • Devendra Jain
    • 1
    Email author
  • Ali Asger Bhojiya
    • 1
    • 5
  • Aradhana Sukhwal
    • 1
  • Suman Sanadhya
    • 1
  • Heena Saheewala
    • 1
  • Gajanand Jat
    • 2
  • Abhijeet Singh
    • 3
  • Santosh Ranjan Mohanty
    • 4
  1. 1.Department of Molecular Biology and Biotechnology, Rajasthan College of AgricultureMaharana Pratap University of Agriculture and TechnologyUdaipurIndia
  2. 2.Department of Soil Science and Agricultural Chemistry, Rajasthan College of AgricultureMaharana Pratap University of Agriculture and TechnologyUdaipurIndia
  3. 3.Department of BiosciencesManipal University JaipurJaipurIndia
  4. 4.All India Network Project on Soil Biodiversity-BiofertilizersICAR-Indian Institute of Soil ScienceBhopalIndia
  5. 5.Department of Agriculture and Veterinary SciencesMewar UniversityChittaurgarhIndia

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