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3 Biotech

, 9:425 | Cite as

Rapid detection of Salmonella enterica in raw milk samples using Stn gene-based biosensor

  • Kritika Saini
  • Ankur Kaushal
  • Shagun Gupta
  • Dinesh KumarEmail author
Original Article
  • 30 Downloads

Abstract

In this study, a DNA-based nanosensor using specific NH2 labeled single standard probe was developed against stn gene of Salmonella enterica in milk samples. The single-stranded DNA probe was immobilized on carboxylated multiwalled carbon nanotube and gold nanoparticle (c-MWCNT/AuNP) electrode using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC): N-hydroxy succinimide-based cross-linking chemistry. Electrochemical characterization was performed using cyclic voltammetry (CV) and Differential Pulse Voltammetry (DPV) techniques. The electrode surface at each step of fabrication was characterized using scanning electron microscopy. The sensitivity and lower limit of detection were found to be 728.42 (μA/cm2)/ng and 1.8 pg/6 μl (0.3 pg/ml), respectively, with regression coefficient (R2) of 0.843 using DPV. The sensor was further validated using raw and artificial milk samples, and results were compared with conventional methods of detection. The developed sensor was found to be highly sensitive and stable up to 6 months, with only 10% loss of initial peak current in CV analysis on storage at 4 °C.

Keywords

Salmonella enterica Foodborne illness stn gene Electrochemical biosensor 

Notes

Acknowledgements

The authors are thankful to Shoolini University, Solan, Himachal Pradesh for providing the facility to carry out the present research work. In addition, we would like to acknowledge scientific writing cell for the support provided towards language editing of the manuscript.

Compliances with ethical standards

Conflict of interest

There is no conflict of interest for authorship or related to any other context between authors.

Ethical standards

The authors have complied and worked within standard ethical norms.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Shoolini University of Biotechnology and Management SciencesDistrict SolanIndia
  2. 2.Amity UniversityGurgaonIndia

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