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3 Biotech

, 9:424 | Cite as

Ligninolytic behavior of the white-rot fungus Stereum ostrea under influence of culture conditions, inducers and chlorpyrifos

  • B. S. Shanthi Kumari
  • K. Praveen
  • K. Y. Usha
  • Kanderi Dileep Kumar
  • G. Praveen Kumar Reddy
  • B. Rajasekhar ReddyEmail author
Original Article
  • 28 Downloads

Abstract

The production of three ligninolytic enzymes, laccase (LAC), manganese peroxidase (MnP) and lignin peroxidase (LiP) by the white-rot fungus, Stereum ostrea, was significantly more in Koroljova liquid medium in the presence of chlorpyrifos under shaking conditions than under stationary conditions. These enzymes were secreted into the broth to the extent of 214.37, 82.75 and 8.05 U/ml under influence of chlorpyrifos on 10th day of incubation in comparison with 138.06, 51.85 and 6.44 U/ml, respectively, under similar conditions in control. Maximum production of LAC, MnP and LiP on liquid medium with/without chlorpyrifos under stationary conditions did not exceed 80–85, 33–40, 0.6–0.7 U/ml, respectively. Among lignosulfonic acid, veratryl alcohol (VA), gallic acid (GA) and tannic acid tested, GA induced maximum production of LAC (300.53 U/ml) and MnP (181.66 U/ml) after 10 days of growth in the presence of chlorpyriphos, while maximum LiP (1.134 U/ml) was produced when grown with the inducer VA during this period. Our data suggest that chlorpyrifos and inducers interacted positively in producing higher amounts of the ligninolytic enzymes in S. ostrea.

Keywords

Chlorpyrifos Inducers LAC Ligninolytic enzymes LiP MnP Stereum ostrea White-rot fungi 

Notes

Acknowledgements

This work was supported with grants from a major research Project [F.No. 42-476/2013(SR) dated 22-03-2013], UGC, New Delhi and the assistance in the form of project fellow in the project to Miss. B.S. Shanthi Kumari is duly acknowledged.

Compliance with ethical standards

Competing interest

Among the authors, there is no competing interest in the publication of the manuscript.

Supplementary material

13205_2019_1955_MOESM1_ESM.docx (478 kb)
Photo 1 Growth of S. ostrea under stationary conditions on 10th incubation. Photo 2 Growth of S. ostrea under shaking conditions on 10th day of incubation (DOCX 477 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of MicrobiologySri Krishnadevaraya UniversityAnantapuramuIndia

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