3 Biotech

, 9:405 | Cite as

Antimicrobial potential of endophytic fungi from Astragalus chinensis

  • Peiji Liu
  • Dekui Zhang
  • Ruirui Shi
  • Zhengyou YangEmail author
  • Fengchun ZhaoEmail author
  • Yuan TianEmail author
Original Article


The purpose of the present study was to discover antimicrobial endophytic fungi from Astragalus chinensis. Three fungal endophytes with antibacterial activity were isolated and determined as Chaetomium sp. HQ-1, Fusarium sp. HQ-7 and Fusarium sp. HQ-9 based on the neighbor-joining phylogenetic tree. Chaetomium sp. HQ-1 showed the best antibiotic potential and was thus selected for large-scale fermentation. Bioactivity-directed separation of ME fermentation of strain HQ-1 led to the discovery of three compounds, which were identified as differanisole A (1), 2,6-dichloro-4-propylphenol (2) and 4,5-dimethylresorcinol (3), from the HR–ESI–MS and NMR data analysis. All three compounds exhibited moderate antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, and methicillin-resistant S. aureus, with MIC values ranging from 16 to 128 μg/mL. Compounds 1 and 3 also displayed promising antifungal activity against Selerotium rolfsii with IC50 values of less than 16 and 32 μg/mL, respectively, which were comparable to that of actidione (8 μg/mL). The findings of the present study suggest that the endophytic fungi from A. chinensis have the potential to be used as bactericides and fungicides.


Endophyte Astragalus chinensis Chaetomium sp. Antibiotic Differanisole A 



This work was supported by the National Natural Science Foundation of China (21602152), Shandong Provincial Natural Science Foundation (ZR2016BB01), Shandong Provincial Key Laboratory of Agricultural Microbiology Open Fund (SDKL2017015).

Author contributions

PL, DZ and RS performed the experiments and analyzed data. ZY and FZ edited the manuscript. YT designed the experiments. All authors revised the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary material

13205_2019_1948_MOESM1_ESM.docx (411 kb)
Supplementary material 1 (DOCX 411 kb)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Microbiology, College of Life Science, Key Laboratory for Agriculture MicrobiologyShandong Agricultural UniversityTaianChina
  2. 2.College of Life ScienceShandong First Medical University and Shandong Academy of Medical SciencesTaianChina

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