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3 Biotech

, 9:402 | Cite as

Antibiofilm potential of Seabuckthorn silver nanoparticles (SBT@AgNPs) against Pseudomonas aeruginosa

  • Vijay Singh Gondil
  • Thiyagarajan Kalaiyarasan
  • Vijay K. Bharti
  • Sanjay ChhibberEmail author
Original Article

Abstract

In era of antibiotic resistance, antibacterial silver nanoparticles are considered as potential alternative therapeutic agent to combat drug resistant pathogens. The aim of present study was to evaluate the antibacterial, antibiofilm and biocompatible potential of green synthesized Seabuckthorn silver nanoparticles (SBT@AgNPs). In the study, antibacterial efficiency of SBT@AgNPs was studied against Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and methicillin resistant Staphylococcus aureus. SBT@AgNPs were found to possess high antibacterial activity which was indicated in terms of low minimum inhibitory and bactericidal concentrations (2–4 µg/ml) obtained against test pathogens. Anti-biofilm activity of SBT@AgNPs on young as well as mature P. aeruginosa biofilms was also evaluated. SBT@AgNPs were able to eradicate the P. aeruginosa biofilms, which was further confirmed by field emission scanning electron microscopy and confocal laser scanning microscopy. Quorum sensing assay also revealed the quorum quenching activity of SBT@AgNPs. Biocompatibility and cytocompatibility results demonstrated SBT@AgNPs to exhibit first-rate non-toxicity as no membrane damage on RBCs or detrimental morphology variation was seen in human dermal fibroblast. LC–MS analysis was also carried out to analyze the potential antibacterial chemical compounds present in aqueous extract of Seabuckthorn leaves. To the best of our knowledge this is first study in which green synthesized silver nanoparticles were exploited to eradicate young as well as mature biofilms of P. aeruginosa. Results showed that SBT@AgNPs are highly antibacterial, antibiofilm, nontoxic in nature and consequently can aid in biomedical applications.

Keywords

Seabuckthorn silver nanoparticles Biofilms Pseudomonas aeruginosa Antibiotic resistance 

Notes

Acknowledgements

Mr. Vijay Singh Gondil is highly grateful to University Grants Commission (UGC), New Delhi, India for financial assistance in form of JRF/SRF. Kalaiyarasan and Vijay K Bharti are thankful to Defence Research and Developmental Organization (DRDO), New Delhi, India, for financial support, and Dr. Johnson, Center for Nanoscience and nanotechnology, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, India, for providing cell culture facility to carry out cytotoxicity studies. The authors duly acknowledge Prof. Kusum Harjai, Ms. Surekha, Department of Microbiology, Panjab University, Chandigarh for quorum sensing studies, DST PURSE Project Implementation Group, Mangalore University, Karnataka, India for LC–MS facility, and Dr. Garima for help during results analysis, SAIF-CIL, Panjab University, Chandigarh for FESEM and EDS facility.

Funding

The authors have no funding to report.

Compliance with ethical standards

Conflict of interest

Authors declare no conflict(s) of interest.

Supplementary material

13205_2019_1947_MOESM1_ESM.docx (224 kb)
Supplementary material 1 (DOCX 223 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Microbiology, Basic Medical SciencesPanjab UniversityChandigarhIndia
  2. 2.Defence Institute of High Altitude Research (DIHAR), DRDOLeh-LadakhIndia

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