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3 Biotech

, 9:417 | Cite as

Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33

  • Pisit Thamvithayakorn
  • Cherdchai Phosri
  • Nipon Pisutpaisal
  • Sukhumaporn Krajangsang
  • Anthony J. S. Whalley
  • Nuttika SuwannasaiEmail author
Original Article
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Abstract

Oil palm decanter cake (OPDC) in the current study was converted to valuable products as laccase and manganese peroxidase (MnP) by an undescribed strain of the white-rot fungus, Pseudolagarobasidium sp. PP17-33. The optimization to enhance the production of enzymes through solid-state fermentation was performed using Plackett–Burman design and response surface methodology. The highest observed laccase was 5.841 U/gds and observed MnP was 5.156 U/gds, which enhanced yield by 2.59-fold and 1.94-fold from the non-optimization. The optimized medium (mg/g of OPDC) consisted of 0.852 mg CuSO4·5H2O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH2PO4, 1.5 mg MgSO4·7H2O, 0.01 mg FeSO4·7H2O, 0.15 mg MnSO4·H2O, 0.01 mg ZnSO4·7H2O and 0.3 mg Tween 80 (pH 5.0) when incubated at 30 °C for 7 days. The most significant variables of laccase and MnP productions were CuSO4·5H2O and glucose concentrations. This study is the first to report on the production of ligninolytic enzymes from OPDC waste using white-rot fungi. In addition, five different white-rot fungi, Coriolopsis aspera, C. retropicta, Dentipellis parmastoi, Nigroporus vinosus and Tyromyces xuchilensis, are newly observed producers of ligninolytic enzymes in Thailand. The results obtained from this study are significant not only for agro-industrial waste management but also for value-added enzyme production.

Keywords

Laccase Manganese peroxidase Oil palm decanter cake Pseudolagarobasidium Response surface methodology 
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Notes

Acknowledgements

The authors are thankful to the Biodiversity-based Economy Development Office (BEDO-Thailand) for funding the research activities (Grant no. 37/2561) and The King Mongkut’s University of Technology North Bangkok (Grant no. KMUTNB-62-KNOW-07). We would like to thank Assistant Professor Dr. Rungpetch Khaengraeng for the supporting of fungal collections.

Author contributions

PT, CP, NP and NS designed the experiments and drafted the manuscript. PT, CP and NS collected the fungal samples. PT, CP, NS and AJSW classified and identified the fungal species. PT, SK and NS analysed the data using statistical methods. AJSW edited and modified the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declared no conflict of interest.

Supplementary material

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Supplementary material 1 (DOCX 5762 kb)
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Supplementary material 4 (DOCX 12 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Biology, Faculty of ScienceSrinakharinwirot UniversityBangkokThailand
  2. 2.Department of Biology, Faculty of ScienceNakhon Phanom UniversityNakhon PhanomThailand
  3. 3.Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied ScienceKing Mongkut’s University of Technology North BangkokBangkokThailand
  4. 4.Department of Microbiology, Faculty of ScienceSrinakharinwirot UniversityBangkokThailand
  5. 5.School of Pharmacy and Biomolecular ScienceLiverpool John Moore UniversityLiverpoolUK

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