Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33
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Oil palm decanter cake (OPDC) in the current study was converted to valuable products as laccase and manganese peroxidase (MnP) by an undescribed strain of the white-rot fungus, Pseudolagarobasidium sp. PP17-33. The optimization to enhance the production of enzymes through solid-state fermentation was performed using Plackett–Burman design and response surface methodology. The highest observed laccase was 5.841 U/gds and observed MnP was 5.156 U/gds, which enhanced yield by 2.59-fold and 1.94-fold from the non-optimization. The optimized medium (mg/g of OPDC) consisted of 0.852 mg CuSO4·5H2O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH2PO4, 1.5 mg MgSO4·7H2O, 0.01 mg FeSO4·7H2O, 0.15 mg MnSO4·H2O, 0.01 mg ZnSO4·7H2O and 0.3 mg Tween 80 (pH 5.0) when incubated at 30 °C for 7 days. The most significant variables of laccase and MnP productions were CuSO4·5H2O and glucose concentrations. This study is the first to report on the production of ligninolytic enzymes from OPDC waste using white-rot fungi. In addition, five different white-rot fungi, Coriolopsis aspera, C. retropicta, Dentipellis parmastoi, Nigroporus vinosus and Tyromyces xuchilensis, are newly observed producers of ligninolytic enzymes in Thailand. The results obtained from this study are significant not only for agro-industrial waste management but also for value-added enzyme production.
KeywordsLaccase Manganese peroxidase Oil palm decanter cake Pseudolagarobasidium Response surface methodology
The authors are thankful to the Biodiversity-based Economy Development Office (BEDO-Thailand) for funding the research activities (Grant no. 37/2561) and The King Mongkut’s University of Technology North Bangkok (Grant no. KMUTNB-62-KNOW-07). We would like to thank Assistant Professor Dr. Rungpetch Khaengraeng for the supporting of fungal collections.
PT, CP, NP and NS designed the experiments and drafted the manuscript. PT, CP and NS collected the fungal samples. PT, CP, NS and AJSW classified and identified the fungal species. PT, SK and NS analysed the data using statistical methods. AJSW edited and modified the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declared no conflict of interest.
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