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3 Biotech

, 9:414 | Cite as

Essential oil yield estimation by Gas chromatography–mass spectrometry (GC–MS) after Methyl jasmonate (MeJA) elicitation in in vitro cultivated tissues of Coriandrum sativum L.

  • Muzamil Ali
  • Abdul MujibEmail author
  • Basit Gulzar
  • Nadia Zafar
Original Article
First Online:
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Abstract

Coriandrum sativum is an important spice plant known for its unique fragrance. Coriander oil is also one of the major essential oils in world global market. The oil yield varies with different coriander varieties; and the content and quality of oil is governed by several factors. In recent times, a variety of technologies have been exploited to improve phyto-compounds including essential oils. In this present study, Methyl jasmonate (MeJA) was amended in medium and the yield of essential oil was measured and compared in different cultivating tissues. The cultured tissues were nonembryogenic callus and embryogenic tissues (induction, proliferation and maturation stages of embryos). MeJA acts as a signaling molecule in accumulating secondary metabolites. Four different MeJA treatments i.e. T1 = 50, T2 = 100, T3 = 150 and T4 = 200 μM, along with a control (T0) were used and the yield of coriander essential oil was estimated in different in vitro cultivating tissues by using Gas chromatography–mass spectrometry (GC–MS). The addition of MEJA enriched essential oil yield, maximum oil being in maturation stage of embryos at T3 (150 μM). Other added treatments also had varied stimulatory role. The addition of MeJA induced stress as the stress marker enzymes like superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) content were high compared to non treated tissue (T0). In T4, the CAT activity was maximum i.e. 5.83 and 6.28 mg−1 protein min−1 in Co-1 and RS respectively in matured somatic embryos. The SOD activity was also high at maturation stage of embryos at T4 (5.3 mg−1 protein min−1 in RS). The APX activity on the other, was high (3.32 mg−1 protein min−1) in induction stage of embryogenesis at T3. The comparative biochemical (sugar, protein and proline) analyses of tissues were performed and presented that had high and low essential oil. MeJA induced stress may help in accumulating essential oils in C. sativum.

Keywords

Callus Embryogenic callus Elicitor Essential oil Methyl jasmonate 
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Notes

Acknowledgements

The first author is thankful to University Grant Commission for awarding Junior Research Fellowship. The authors are also grateful to the Department of Botany, Jamia Hamdard for providing necessary facility and to Central Instrumentation facilities for providing other help.

Author contributions

MA performed all the experimental works; Other scientists involved in this study helped in designing experiments, preparing tables, figures and photoplates; and AM supervised and edited manuscript for final submission.

Compliance with ethical standards

Conflict of interest

Authors declare that there is no conflict of interest.

Ethical approval

This article does not require any experiment or study with human participants or animals.

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© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Cellular Differentiation and Molecular Genetics Section, Department of BotanyJamia HamdardNew DelhiIndia

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