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3 Biotech

, 9:407 | Cite as

Hairy root induction and Farnesiferol B production of endemic medicinal plant Ferula pseudalliacea

  • Abedin Khazaei
  • Bahman BahramnejadEmail author
  • Ali-Akbar Mozafari
  • Dara Dastan
  • Sima Mohammadi
Original Article
  • 43 Downloads

Abstract

The effects of medium, gibberellic acid (GA3) and stratification treatments on the seed germination of Ferula pseudalliacea were evaluated. Filter paper medium, 500 micro molar GA3 and 8 week chilling treatment were resulted in significantly more seed germination than others. F. pseudalliacea was also transformed by Agrobacterium rhizogenes. Explants from young leaves, stems, cotyledon, and embryo were inoculated with A. rhizogenes strains ATCC 15834, 1724, A4, LB9402 and Ar318. Hairy roots were induced only from 10 to 12-days embryo explants using strains ATCC 15824 and 1724. Although, the transformation efficiency of ATCC 15834 (4%) strain was higher than 1724 (2%). Maximum hairy root transformation frequency (25%) was obtained in infection time of 10 min compared to that of 20 (20%) and 30 (5%) min. In addition, the transformation rate was significantly higher at the inoculation time of 72 h (29%) compared to that of 48 h (22%) and 24 h (6%). Transgenic hairy root lines were confirmed by PCR amplification of rolB gene. Hairy root lines were produced higher biomass in half B5 medium compared to that of half MS medium. Hairy roots lines from the strain ATCC 15834 produced more hairy root numbers and fresh and dried biomass compared to that of the strain 1724. Analyses of transgenic hairy root and natural roots extracts using HPLC showed that all the hairy root lines produced farnesiferol B.

Keywords

Seed germination Agrobacterium rhizogenes Transformation Hairy root induction Farnesiferol B 

Notes

Acknowledgements

This work was supported by the University of Kurdistan. The authors would like to thank Dr. Mirzaghaderi, University of Kurdistan, for his help in counting of mitotic chromosomes.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abd El-Razek MH (2003) Terpenoid coumarins of the genus Ferula. Heterocycles 60:689–716Google Scholar
  2. Alpizar E, Dechamp E, Espeout S, Royer M, Lecouls A-C, Nicole M, Bertrand B, Lashermes P, Etienne H (2006) Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots. Plant Cell Rep 25:959–967PubMedGoogle Scholar
  3. Asghari J, Atabaki V, Baher E, Mazaheritehrani M (2016) Identification of sesquiterpene coumarins of oleo-gum resin of Ferula assa-foetida L. from the Yasuj region. Nat Prod Res 30:350–353PubMedGoogle Scholar
  4. Bahramnejad B, Naji M, Bose R, Jha S (2019) A critical review on use of Agrobacterium rhizogenes and their associated binary vectors for plant transformation. Biotechnol Adv S0734–9750(19):30095.  https://doi.org/10.1016/j.biotechadv.2019.06.004 CrossRefGoogle Scholar
  5. Bamehr H, Saidijam M, Dastan D, Amini R, Pourjafar M, Najafi R (2019) Ferula pseudalliacea induces apoptosis in human colorectal cancer HCT-116 cells via mitochondria-dependent pathway. Arch Physiol Biochem 125:284–291PubMedGoogle Scholar
  6. Bansal M, Kumar A, Reddy MS (2014) Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside A’production from Bacopa monnieri (L.) Wettst. Acta Physiol Plant 36:2793–2801Google Scholar
  7. Barthomeuf C, Lim S, Iranshahi M, Chollet P (2008) Umbelliprenin from Ferula szowitsiana inhibits the growth of human M4Beu metastatic pigmented malignant melanoma cells through cell-cycle arrest in G1 and induction of caspase-dependent apoptosis. Phytomedicine 15:103–111PubMedGoogle Scholar
  8. Brijwal L, Tamta S (2015) Agrobacterium rhizogenes mediated hairy root induction in endangered Berberis aristata DC. SpringerPlus 4:443PubMedPubMedCentralGoogle Scholar
  9. Dastan D, Salehi P, Gohari AR, Zimmermann S, Kaiser M, Hamburger M, Khavasi HR, Ebrahimi SN (2012) Disesquiterpene and sesquiterpene coumarins from Ferula pseudalliacea, and determination of their absolute configurations. Phytochemistry 78:170–178PubMedGoogle Scholar
  10. Dastan D, Salehi P, Ghanati F, Gohari AR, Maroofi H, Alnajar N (2014) Phytotoxicity and cytotoxicity of disesquiterpene and sesquiterpene coumarins from Ferula pseudalliacea. Ind Crop Prod 55:43–48Google Scholar
  11. Dastan D, Salehi P, Aliahmadi A, Gohari AR, Maroofi H, Ardalan A (2016) New coumarin derivatives from Ferula pseudalliacea with antibacterial activity. Nat Prod Res 30:2747–2753PubMedGoogle Scholar
  12. DeBoer KD, Lye JC, Aitken CD, Su AK-K, Hamill JD (2009) The A622 gene in Nicotiana glauca (tree tobacco): evidence for a functional role in pyridine alkaloid synthesis. Plant Mol Biol 69:299–312PubMedGoogle Scholar
  13. Eigner D, Scholz D (1999) Ferula asa-foetida and Curcuma longa in traditional medical treatment and diet in Nepal. J Ethnopharmacol 67:1–6PubMedGoogle Scholar
  14. El-Razek MHA, Ohta S, Ahmed AA, Hirata T (2001) Sesquiterpene coumarins from the roots of Ferula assa-foetida. Phytochemistry 58:1289–1295Google Scholar
  15. Golmohammadi F (2013) Medical plant of Ferula assa-foetida and its cultivating, main characteristics and economical importance in South Khorasan province-east of Iran. TJEAS 3:2334–2346Google Scholar
  16. Grzegorczyk I, Królicka A, Wysokińska H (2006) Establishment of Salvia officinalis L. hairy root cultures for the production of rosmarinic acid. Zeitschrift für Naturforschung C 61:351–356Google Scholar
  17. Gudarzi H, Salimi M, Irian S, Amanzadeh A, Mostafapour Kandelous H, Azadmanesh K, Salimi M (2015) Ethanolic extract of Ferula gummosa is cytotoxic against cancer cells by inducing apoptosis and cell cycle arrest. Nat Prod Res 29:546–550PubMedGoogle Scholar
  18. Hassani S, Saboora A, Radjabian T, Fallah Husseini H (2009) Effects of temperature, GA3 and cytokinins on breaking seed dormancy of Ferula assa-foetida L. IJSTS 33:75–85Google Scholar
  19. Hosseini SM, Bahramnejad B, Douleti Baneh H, Emamifar A, Goodwin PH (2017) Hairy root culture optimization and resveratrol production from Vitis vinifera subsp. sylvesteris. World J Microbiol Biotechnol 33:67–76PubMedGoogle Scholar
  20. Iranshahi M, Arfa P, Ramezani M, Jaafari MR, Sadeghian H, Bassarello C, Piacente S, Pizza C (2007) Sesquiterpene coumarins from Ferula szowitsiana and in vitro antileishmanial activity of 7-prenyloxycoumarins against promastigotes. Phytochemistry 68:554–561PubMedGoogle Scholar
  21. Kajikawa M, Hirai N, Hashimoto T (2009) A PIP-family protein is required for biosynthesis of tobacco alkaloids. Plant Mol Biol 69:287PubMedGoogle Scholar
  22. Kanani MR, Rahiminejad MR, Sonboli A, Mozaffarian V, Kazempour Osaloo S, Nejad Ebrahimi S (2011) Chemotaxonomic significance of the essential oils of 18 Ferula species (Apiaceae) from Iran. Chem Biodivers 8:503–517PubMedGoogle Scholar
  23. Kapoor L (2000) Handbook of ayurvedic medicinal plants: Herbal reference library. CRC Press, Boca RatonGoogle Scholar
  24. Keshtkar A, Keshtkar H, Razavi S, Dalfardi S (2008) Methods to break seed dormancy of Astragalus cyclophyllon. Afr J Biotechnol 7:3874–3877Google Scholar
  25. Lee J-H, Choi S, Lee Y, Lee H-J, Kim K-H, Ahn K-S, Bae H, Lee H-J, Lee E-O, Ahn K-S (2010) Herbal compound farnesiferol C exerts antiangiogenic and antitumor activity and targets multiple aspects of VEGFR1 (Flt1) or VEGFR2 (Flk1) signaling cascades. Mol Can Ther 9:389–399Google Scholar
  26. Lima JE, Benedito VA, Figueira A, Peres LEP (2009) Callus, shoot and hairy root formation in vitro as affected by the sensitivity to auxin and ethylene in tomato mutants. Plant Cell Rep 28:1169–1177PubMedGoogle Scholar
  27. Liu S, Su L, Liu S, Zeng X, Zheng D, Hong L, Li L (2016) Agrobacterium rhizogenes-mediated transformation of Arachis hypogaea: an efficient tool for functional study of genes. Biotechnol Biotechnol Equip 30:869–878Google Scholar
  28. Moghadam FH, Dehghan M, Zarepur E, Dehlavi R, Ghaseminia F, Ehsani S, Mohammadzadeh G, Barzegar K (2014) Oleo gum resin of Ferula assa-foetida L. ameliorates peripheral neuropathy in mice. J Ethnopharmacol 154:183–189Google Scholar
  29. Murray RDH, Méndez J, Brown SA (1982) The natural coumarins. Wiley, ChichesterGoogle Scholar
  30. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  31. Murthy HN, Dijkstra C, Anthony P, White DA, Davey MR, Power JB, Hahn EJ, Paek KY (2008) Establishment of Withania somnifera hairy root cultures for the production of withanolide A. J Integr Plant Biol 50:975–981PubMedGoogle Scholar
  32. Nadjafi F, Bannayan M, Tabrizi L, Rastgoo M (2006) Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. J Arid Environ 64:542–547Google Scholar
  33. Nazari ZE, Iranshahi M (2011) Biologically active sesquiterpene coumarins from Ferula species. Phytother Res 25:315–323PubMedGoogle Scholar
  34. Nin S, Bennici A, Roselli G, Mariotti D, Schiff S, Magherini R (1997) Agrobacterium-mediated transformation of Artemisia absinthium L. (wormwood) and production of secondary metabolites. Plant Cell Rep 16:725–730PubMedGoogle Scholar
  35. Ono NN, Tian L (2011) The multiplicity of hairy root cultures: prolific possibilities. Plant Sci 180:439–446PubMedGoogle Scholar
  36. Panda BM, Mehta UJ, Hazra S (2017) Optimizing culture conditions for establishment of hairy root culture of Semecarpus anacardium L. 3 Biotech 7:21PubMedPubMedCentralGoogle Scholar
  37. Pimenov MG, Leonov MV (1993) The genera of the Umbelliferae: a nomenclator. Royal Botanic Gardens, KewGoogle Scholar
  38. Pimenov M, Leonov M, Van Wyk B-E, Tilney P (2004) Asia, the continent with the highest Umbelliferae biodiversity. S Afr J Bot 70:417–419Google Scholar
  39. Porter JR, Flores H (1991) Host range and implications of plant infection by Agrobacterium rhizogenes. Crit Rev Plant Sci 10:387–421Google Scholar
  40. Razavi SM, Janani M (2015) A new ester coumarin from Ferula Persica wild, indigenous to Iran. Nat Prod Res 29:717–721PubMedGoogle Scholar
  41. Sattar Z, Iranshahi M (2017) Phytochemistry and pharmacology of Ferula persica Boiss: a review. Iran J Basic Med Sci 20:1–8PubMedPubMedCentralGoogle Scholar
  42. Shahverdi A, Saadat F, Khorramizadeh M, Iranshahi M, Khoshayand M (2006) Two matrix metalloproteinases inhibitors from Ferula persica var. persica. Phytomedicine 13:712–717PubMedGoogle Scholar
  43. Sharafi A, Sohi HH, Azadi P, Sharafi AA (2014) Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi. Physiol Mol Biol Plants 20:257–262PubMedPubMedCentralGoogle Scholar
  44. Sharma P, Khajuria A, Verma S (2013) Genomic DNA extraction from Ferula jaeschkeana Vatke (Apiaceae) optimized for random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) analysis. Proc Natl Acad Sci India Sect B Biol Sci 83:341–345Google Scholar
  45. Sujatha G, Zdravkovic-Korac S, Flamini Calic D, Kumari BR (2013) High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: hairy root production and essential oil analysis. Ind Crops Prod 44:643–652Google Scholar
  46. Tan R-F, Tao J, Li L (2007) Genetic transformation of Ipomoea purpurea mediated by Agrobacterium rhizogenes. Floric Ornam Biotech 1:131–135Google Scholar
  47. Tavassoli P, Afshar Safipour (2018) Influence of different Agrobacterium rhizogenes strains on hairy root induction and analysis of phenolic and flavonoid compounds in marshmallow (Althaea officinalis L.). 3 Biotech 8:351PubMedPubMedCentralGoogle Scholar
  48. Trypsteen M, Van Lijsebettens M, Van Severen R, Van Montagu M (1991) Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea. Plant Cell Rep 10:85–89PubMedGoogle Scholar
  49. Xu H, Zhou X, Lu J, Wang J, Wang X (2006) Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize. Sci Chin Ser C Life Sci 49:305–310Google Scholar
  50. Yao SC, Bai LH, Lan ZZ, Tang MQ, Zhai YJ, Huang H, Wei RC (2016) Hairy root induction and polysaccharide production of medicinal plant Callerya speciosa Champ. Plant Cell Tissue Organ Cult 126:177–186Google Scholar
  51. Yonemitsu H, Shimomura K, Satake M, Mochida S, Tanaka M, Endo T, Kaji A (1990) Lobeline production by hairy root culture of Lobelia inflata L. Plant Cell Rep 9:307–310PubMedGoogle Scholar
  52. Zare A, Solouki M, Omidi M, Irvani N, Oladzad A, Mahdi Nezad N (2011) Effect of various treatments on seed germination and dormancy breaking in Ferula assa-foetida L. (Asafetida), a threatened medicinal herb. Trakia J Sci 9:57–61Google Scholar
  53. Zarei H, Rezaee R, Behravan E, Soltani F, Mosaffa F, Iranshahi M, Behravan J (2013) Diversin, from Ferula diversivittata protects human lymphocytes against oxidative stress induced by H2O2. Natural Prod Res 27:1016–1019Google Scholar
  54. Zargari A (1997) Pharmaceutical plants. Persian Tehran University Press, TehranGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Agronomy and Plant BreedingUniversity of KurdistanSanandajIran
  2. 2.Department of Horticultural Science, Faculty of AgricultureUniversity of KurdistanSanandajIran
  3. 3.Medicinal Plants and Natural Products Research CenterHamadan University of Medical SciencesHamadanIran
  4. 4.Department of Pharmacognosy and Pharmaceutical Biotechnology, School of PharmacyHamadan University of Medical SciencesHamadanIran

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