3 Biotech

, 9:393 | Cite as

Production of humic acids by solid-state fermentation of Trichoderma reesei in raw oil palm empty fruit bunch fibers

  • Maria Paula Cardeal Volpi
  • Ingri Julieth Mancilla Corzo
  • Reinaldo Gaspar BastosEmail author
  • Maria Helena Andrade Santana
Original Article


Humic acids (HA) are organic macromolecules of high structural complexity and are primarily obtained from non-renewable carbon sources such as peat and coal. HA is widely used in agriculture but is known to have therapeutic properties, which are still poorly explored. Previous studies have shown the potential of biotechnological processes in the production of HA in submerged fermentation (SF) and solid-state fermentation (SSF) using pre-treated fibers of oil palm of empty fruit bunch (EFB) for the cultivation of Trichoderma strains. EFB is an agro-industrial residue that is readily available at a low cost. The present study aimed to study the production of HA by Trichoderma reesei in the SSF of raw fibers of EFBs from two different palm oil producers. Profiles of HA production, cellular protein, pH, glucose, moisture, and oxygen transfer were obtained during SSF by EFB with and without lipids, as well as, images by electron microscopy of fibers. Results showed efficient HA production in the raw fibers of the EFBs. HA production followed the cellular protein (6 g HA per 100 g of fibers) evolution of the fermentations in the absence of lipids, while the composition of lipids greatly affected its production. The best HA production (350 mg HA per 100 g of fibers) was obtained from EFB that was richer in lignocellulosics and the residual lipids were similar to the fractions of palm and kernel oils, while EFB with lower lignocellulosic presented a production 110 mg HA per 100 g of fibers.


Humic acids Empty fruit bunch Agroindustrial residue Trichoderma reesei Solid-state fermentation 



The authors are grateful for the support given by School of Chemical Engineering (FEQ/UNICAMP), Agropalma and cooperative of small palm oil producing industries from Muniz Ferreira by supplying of the EFB. This work was supported by the scholarship of the Brazilian National Council for Scientific and Technological Development (CNPq) [Grant number 830535/1999-3].

Compliance with ethical standards

Conflict of interest

The authors have no financial conflicts of interest to declare.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Maria Paula Cardeal Volpi
    • 1
  • Ingri Julieth Mancilla Corzo
    • 1
  • Reinaldo Gaspar Bastos
    • 2
    Email author
  • Maria Helena Andrade Santana
    • 1
  1. 1.Department of Engineering of Materials and Bioprocesses, School of Chemical EngineeringUniversity of CampinasCampinasBrazil
  2. 2.Center of Agricultural SciencesFederal University of São Carlos (UFSCar)ArarasBrazil

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