Enriching LMW-GS alleles and strengthening gluten properties of common wheat through wide hybridization with wild emmer
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Two advanced lines (BAd7-209 and BAd7-213) with identical high-molecular-weight glutenin subunit composition were obtained via wide hybridization between low-gluten cultivar chuannong16 (CN16) and wild emmer D97 (D97). BAd7-209 was better than BAd7-213, and both of them were much better than CN16 in a dough quality test. We found that BAd7-209 had more abundant and higher expression levels of low-molecular-weight glutenin subunit (LMW-GS) proteins than those of BAd7-213. Twenty-nine novel LMW-GS genes at Glu-A3 locus were isolated from BAd7-209, BAd7-213 and their parents. We found that all 29 LMW-GS genes possessed the same primary structure shared by other known LMW-GSs. Twenty-seven genes encode LMW-m-type subunits, and two encode LMW-i-type subunits. BAd7-209 had a higher number of LMW-GS genes than BAd7-213, CN16, and D97. Two wild emmer genes MG574329 and MG574330 were present in the two advanced lines. Most of the LMW-m-type genes showed minor nucleotide variations between wide hybrids and their parents that could be induced through the wide hybridization process. Our results demonstrated that the wild emmer LMW-GS alleles could be feasibly transferred and integrated into common wheat background via wide hybridization and the potential value of the wild emmer LMW-GS alleles in breeding programs designed to improve wheat flour quality.
KeywordsWild emmer wheat Wide hybridization LMW-GS alleles Flour quality
This work was financially supported by the National Natural Science Foundation of China (No. 31571668; No. 31801360), the National Key Research and Development Program of China (No. 2017YFD0100900), the Science & Technology Department of Sichuan Province (No. 2019YJ0435), and the Education Department of Sichuan Province (No. 18ZA0392).
BHW, LH, and LX designed the experiments. FYG, JL, YFW, YRJ, YH, and JSH carried out experiments. LX analyzed the data and drafted the manuscript. QTJ and YLZ provided their constructive comments and suggestions. LH, BHW, and DL revised the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest in the reported research.
- AACC (2000) Approved methods of the American Association of the Cereal Chemists, 10th edn. St. Paul, MNGoogle Scholar
- Butow BJ, Ma W, Gale KR, Cornish GB, Rampling L, Larroque O, Morell MK, Békés F (2003) Molecular discrimination of Bx7 alleles demonstrates that a highly expressed high-molecular-weight glutenin allele has a major impact on wheat flour dough strength. Theor Appl Genet 107:1524–1532CrossRefPubMedGoogle Scholar
- Duan SE, Zhao WM (2004) Rapid separation and SDS-PAGE analysis of wheat glutenin subunits. J Shaanxi Norm Univ 32:77–79Google Scholar
- Hall T (2007) BioEdit, version 7.0.9. Computer program and documentation. lbis Biosciences, CarlsbadGoogle Scholar
- Liu J, Huang L, Wang C, Liu YX, Yan ZH, Wang ZZ, Xiang L, Zhong XY, Gong FY, Zheng YL, Liu DC, Wu BH (2019) Genome-wide association study reveals novel genomic regions associated with high grain protein content in wheat lines derived from wild emmer wheat. Front Plant Sci 10:464CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang HK, Bian Y, Gou XW, Dong YZ, Rustgi S, Zhang BJ, Xu CM, Li N, Qi B, Han FP, von Wettstein D, Liu B (2013) Intrinsic karyotype stability and gene copy number variations may have laid the foundation for tetraploid wheat formation. Proc Natl Acad Sci USA 110:19466–19471CrossRefPubMedGoogle Scholar