Data-independent acquisition of the proteomics of spleens from chickens infected by avian leukosis virus
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Immunosuppression caused by avian leukemia virus J subgroup (ALV-J) infection includes atrophy or regeneration disorders of the lymphoid organs, decreased immune response, and termination of B lymphocyte maturation process and inhibition of T-lymphocyte development. The regulatory mechanism of the related resistance genes and protein expression is not clear. While searching for a molecular marker for the immune response to ALV-J infection, we detected differentially expressed proteins (DEPs) of spleens from chicken infected by ALV-J at 15th day and 30th day by the data-independent acquisition technique. Approximately 220 DEPs from the spleens of chickens infected by ALV-J were detected. To find a relatively stable biomarker molecule, we summarized the DEPs at two timepoints and selected activating signal cointegrator 1 complex subunit 3 (ASCC3), TBC1 domain family member 2 (TBC1D2), MHC class II beta chain 1 (BLB2), ensconsin (MAP7), complement component 1 Q subcomponent B chain (C1QB), and Follistatin-like 1 (FSTL1) from both comparisons for protein interaction network analysis. ASCC3, BLB2, C1QB, and FSTL1 were potential biomarkers for the complex infection mechanism of ALV-J and the dynamic immune mechanism of the body.
KeywordsALV-J Proteomic ASCC3 BLB2 C1QB FSTL1
The Sequencing work was financially supported by the China Agriculture Research System (CARS-41), the Engineering Centre of Chicken Breeding in Guangdong Province (2017-1649) and Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding (2019B030301010). We would like to thank AMERICAN JOURNAL EXPERTS (https://secure.aje.com) for providing linguistic assistance during the preparation of this manuscript.
YW, HL, and QZ conceived and designed the experiments. FY and QJH performed the experiments. FY, CC, BL, FLY and HDY analyzed the data. HYX, DYL, and XLZ contributed the reagents/materials/analysis and tools. FY wrote the paper. YW, HL, and QZ revised the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflicts of interest.
All animal care and experimental procedures were reviewed and approved by the Animal Care and Use Committee (#YYS130125) of the Animal Care Advisory at Sichuan Agricultural University. This study was carried out in strict accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals of the State Council of the People’s Republic of China.
- Jing YY, Li YS, Xin JK, Chai JQ (2014) Co-infection of ALV-J and Salmonella pullorum in laying hens. Pak Vet J 34(3):372–376Google Scholar
- Li J, Ding SC, Cho H, Chung BC, Gale M, Chanda SK, Diamond MS (2013) A short hairpin RNA screen of interferon-stimulated genes identifies a novel negative regulator of the cellular antiviral response. MBio 4(3):e00313–e00385Google Scholar
- Li H, Wang P, Lin L, Shi M, Gu Z, Huang T, Ml Mo, Wei T, Zhang H, Wei P (2019) The emergence of the infection of subgroup J avian leucosis virus escalated the tumour incidence in commercial Yellow chickens in Southern China in recent years. Transbound Emerg Dis 66(1):312–316PubMedCrossRefGoogle Scholar
- Tanaka M, Ozaki S, Kawabata D, Kishimura M, Osakada F, Okubo M, Murakami M, Nakao K, Mimori T (2003) Potential preventive effects of follistatin-related protein/TSC-36 on joint destruction and antagonistic modulation of its autoantibodies in rheumatoid arthritis. Int Immunol 15(1):71–77PubMedCrossRefGoogle Scholar