3 Biotech

, 9:305 | Cite as

Effectiveness of Bacillus pumilus PDSLzg-1, an innovative Hydrocarbon-Degrading Bacterium conferring antifungal and plant growth-promoting function

  • Kun Hao
  • Hidayat Ullah
  • Xinghu Qin
  • Hongna Li
  • Feng Li
  • Ping GuoEmail author
Original Article


Genome of the hydrocarbon-degrading bacterium Bacillus pumilus PDSLzg-1 was analyzed. A group of gene clusters and pathways associated with nitrogen fixation, plant-bacterial interactions, plant growth-promoting hormone synthesis, antibiotics, secondary metabolite, and disease resistance were identified. In addition, 0.06 mg/L of 3-indoleacrylic acid (IAA) and 2 mg/L of gibberellin (GA) were, respectively, detected in PDSLzg-1 fermentation broth by high-performance liquid chromatography (HPLC). Up-regulated expression levels of 11 key genes related to GA and IAA biosynthesis pathways were detected after the induction of 0.2% n-hexadecane. Furthermore, bioassays showed that PDSLzg-1 fermentation could significantly promote the length and biomass of the stems and roots of Triticum aestivum L., while inhibited Colletotrichum truncatum colonization. Results indicated that B. pumilus PDSLzg-1 had plant growth-promoting and antifungal functions, besides its potential applications in phyto-microbial bioremediation combinations for oil-contaminated soil.


Antifungal Plant hormones Phyto-microbial remediation Plant growth-promoting rhizobacteria 



We thank Shan Xue for editing the figures.


This work was supported by the China Major Science and Technology Program for Water Pollution Control and Treatment, the Standardization and Scale of Application of Rural Drinking Water Security Technology (2015ZX07402003-4).

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interests.

Supplementary material

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Kun Hao
    • 1
    • 2
  • Hidayat Ullah
    • 2
    • 3
  • Xinghu Qin
    • 4
  • Hongna Li
    • 1
  • Feng Li
    • 1
  • Ping Guo
    • 1
    Email author
  1. 1.Agricultural Clean Watershed Research Group, Institute of Environment and Sustainable Development in AgricultureChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  3. 3.Department of AgricultureThe University of SwabiSwabiPakistan
  4. 4.School of BiologyUniversity of St AndrewsSt AndrewsUK

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