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3 Biotech

, 9:308 | Cite as

Amelioration of antioxidant potential, toxicity, and antihyperglycemic activity of Hippophae salicifolia D. Don leaf extracts in alloxan-induced diabetic rats

  • Sushil Kumar Middha
  • Talambedu Usha
  • Bharat Chandra Basistha
  • Arvind Kumar GoyalEmail author
Original Article
  • 31 Downloads

Abstract

Efficacy of several plant extracts in the clinical research for modulating oxidative stress correlated with diabetes mellitus (DM) is well documented. In the present study, we investigated the in vitro antioxidant activity, toxicity, and anti-diabetic activity of methanolic extract of Hippophae salicifolia leaves in normal and alloxan-induced diabetic wistar rats. H. salicifolia leaves were found to be rich in antioxidants. The acute toxicity test of methanolic extract of H. salicifolia leaves revealed that the median lethal dose (LD50) was found to be 3.92 g/kg body weight in mice. Administration of H. salicifolia leaves at 200 mg/kg and 400 mg/kg in alloxan-induced diabetic rats illustrated significant reduction (22% and 39%, respectively) in fasting blood glucose compared to diabetic control. Both the doses were found to be effective when compared to diabetic rats. The Hippophae-treated diabetic rats showed significant increase in the endogenous antioxidant enzymes, superoxide dismutase (50% and 74%, respectively), glutathione peroxidase (57% and 41%, respectively) and decrease in malondialdehyde (33% and 15%, respectively) levels. These results suggested that the methanolic leaf extract of H. salicifolia enhanced the antioxidant defence against reactive oxygen species produced under hyperglycaemic conditions.

Keywords

Hippophae salicifolia Antioxidants Diabetes Toxicology Flavonoids Polyphenols Sea buckthorn 

Notes

Acknowledgements

Authors are grateful to Biotechnology-Skill Enhancement program, under Govt. of Karnataka at Maharani Lakshmi Ammanni College for Women, for funding animal house facility to carry out this study.

Author contribution

TU and AKG performed the experiments, analyzed the data and prepared the manuscript. BCB procured and prepared the sample and wrote a part of the manuscript. SKM conceived the idea, designed and coordinated the experiments, improved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in the studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Supplementary material

13205_2019_1840_MOESM1_ESM.jpg (42 kb)
DPPH scavenging activity of H. salicifolia compared to standard ascorbic acid (JPEG 41 kb)

References

  1. Ahmed D, Kumar V, Verma A, Gupta PS, Kumar H, Dhingra V, Mishra V, Sharma M (2014) Anti-diabetic, renal/hepatic/pancreas/cardiac protective and antioxidant potential of methanol/dichloromethane extract of Albizzia Lebbeck Benth. stem bark (ALEx) on streptozotocin induced diabetic rats. BMC Complement Altern Med 14:243CrossRefGoogle Scholar
  2. American Diabetes Association (2014) Diagnosis and classification of diabetes mellitus. Diabetes Care 37(Suppl 1):S81–S90CrossRefGoogle Scholar
  3. Arimboor R, Kumar KS, Arumughan C (2008) Simultaneous estimation of phenolic acids in sea buckthorn (Hippophaë rhamnoides) using RP-HPLC with DAD. J. Pharm. Biomed. Anal. 47(1):31–38CrossRefGoogle Scholar
  4. Asgary S, Rahimi P, Mahzouni P, Madani H (2012) Antidiabetic effect of hydroalcoholic extract of Carthamus tinctorius L. in alloxan-induced diabetic rats. J Res Med Sci 17(4):386–392PubMedPubMedCentralGoogle Scholar
  5. Bazargani YT, de Boer A, Leufkens HGM, Mantel-Teeuwisse AK (2014) Selection of essential medicines for diabetes in low and middle income countries: a survey of 32 national essential medicines lists. PLoS ONE 9(9):e106072CrossRefGoogle Scholar
  6. Cao QH, Qu WJ, Deng YX, Zhang ZC, Niu W, Pan YF (2003) Effects of flavonoids from the seed and fruit residue of Hippophae rhamnoides L. on glycometabolism in mice. J Chin Med Mater 26:735–737Google Scholar
  7. Chang CLT, Lin Y, Bartolome AP, Chen YC, Chiu SC, Yang WC (2013) Herbal therapies for type 2 diabetes mellitus: chemistry, biology, and potential application of selected plants and compounds. Evid-Based Complement Altern Med 2013:378657Google Scholar
  8. Choubey S, Varughese LR, Kumar V, Beniwal V (2015) Medicinal importance of gallic acid and its ester derivatives: a patent review. Pharm Pat Anal 4(4):305–315CrossRefGoogle Scholar
  9. Conlee KM, Stephens ML, Rowan AN, King LA (2005) Carbon dioxide for euthanasia: concerns regarding pain and distress, with special reference to mice and rats. Lab. Anim. 39:137–161CrossRefGoogle Scholar
  10. Dardano A, Penno G, Del Prato S, Miccoli R (2014) Optimal therapy of type 2 diabetes: a controversial challenge. Aging (Albany NY) 6(3):187–206CrossRefGoogle Scholar
  11. Dey SK, Middha SK, Usha T, Brahma BK, Goyal AK (2018) Antidiabetic activity of giant grass Bambusa tulda. Bangladesh J Pharmacol 13(2):134–136CrossRefGoogle Scholar
  12. Flohe L, Gunzler WA (1984) Assays of glutathione peroxidase. Method Enzymol 105:114–121CrossRefGoogle Scholar
  13. Foti MC (2007) Antioxidant properties of phenols. J. Pharm. Pharmacol. 59(12):1673–1685CrossRefGoogle Scholar
  14. Geetha S, Ram MS, Mongia SS, Singh V, Ilavazhagan G, Sawhney RC (2003) Evaluation of antioxidant activity of leaf extract of Seabuckthorn (Hippophae rhamnoides L.) on chromium (VI) induced oxidative stress in albino rats. J. Ethnopharmacol. 87:247–251CrossRefGoogle Scholar
  15. Goyal AK, Middha SK, Sen A (2010) Evaluation of the DPPH radical scavenging activity, total phenols and antioxidant activities in Indian wild Bambusa vulgarisVittata” methanolic leaf extract. J Nat Pharm 1(1):40–45CrossRefGoogle Scholar
  16. Goyal AK, Basistha BC, Sen A, Middha SK (2011) Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India. Funct. Plant Biol. 38:697–701CrossRefGoogle Scholar
  17. Goyal AK, Mishra T, Bhattacharya M, Kar P, Sen A (2013) Evaluation of phytochemical constituents and antioxidant activity of selected actinorhizal fruits growing in the forests of North-East India. J. Biosci. 38(4):797–803CrossRefGoogle Scholar
  18. Goyal AK, Middha SK, Usha T, Sen A (2017) Analysis of toxic, antidiabetic and antioxidant potential of Bambusa balcooa Roxb leaf extracts in alloxan-induced diabetic rats. 3 Biotech 7(2):120CrossRefGoogle Scholar
  19. Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE (2014) Global estimates of diabetes prevalence for 2013 and projection for 2035. Diabetes Res. Clin. Pract. 103(2):137–149CrossRefGoogle Scholar
  20. Guliyev VB, Gul M, Yildirim A (2004) Hippophae rhamnoides L.:chromatographic methods to determine chemical composition, use in traditional medicine and pharmacological effects. J. Chromatogr. B 812:291–307CrossRefGoogle Scholar
  21. Kharroubi AT, Darwish HM (2015) Diabetes mellitus: the epidemic of the century. World J Diabetes 6(6):850–867CrossRefGoogle Scholar
  22. King H, Aubert R, Herman W (1998) Global burden of diabetes, 1995-2025, prevalence, numerical estimates and projections. Diabetes Care 21:1414–1431CrossRefGoogle Scholar
  23. Loomis TA, Hayes AW (1996) Loomis’s essentials of toxicology. Academic press, California, pp 208–245Google Scholar
  24. Mazzari ALDA, Prieto JM (2014) Herbal medicines in Brazil: pharmacokinetic profile and potential herb-drug interactions. Front Pharmacol 5:162CrossRefGoogle Scholar
  25. McLellan KCP, Wyne K, Villagomez ET, Hsueh WA (2014) Therapeutic interventions to reduce the risk of progression from prediabetes to type 2 diabetes mellitus. Ther. Clin. Risk Manag. 10:173–188Google Scholar
  26. Middha SK, Bhattacharjee B, Saini D, Baliga MS, Nagaveni MB, Usha T (2011) Protective role of Trigonella foenum graceum extract against oxidative stress in hyperglycemic rats. Eur Rev Med Pharmacol Sci 15(4):427–435PubMedGoogle Scholar
  27. Middha SK, Usha T, Ravikiran T (2012) Influence of Punica granatum L. on region specific responses in rat brain during alloxan-induced diabetes. Asian Pac J Trop Biomed 2:S905–S909CrossRefGoogle Scholar
  28. Middha SK, Goyal AK, Faizan SA, Sanghamitra N, Basistha BC, Usha T (2013) In silico–based combinatorial pharmacophore modelling and docking studies of GSK-3β and GK inhibitors of Hippophae. J. Biosci. 38(4):805–814CrossRefGoogle Scholar
  29. Middha SK, Goyal AK, Lokesh P, Yardi V, Mojamdar L, Keni DS, Babu D, Usha T (2015) Toxicological evaluation of Emblica officinalis fruit extract and its anti-inflammatory and free radical scavenging properties. Pharmacogn Mag 11:427–433CrossRefGoogle Scholar
  30. Middha SK, Usha T, Pande V (2016) Insights into the causes and anti-hyperglycemic effects of Punica granatum rind in alloxan-induced diabetic rats. Chiang Mai J Sci 43:112–122Google Scholar
  31. Misra HP, Fridovich I (1972) The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 247:3170–3175PubMedGoogle Scholar
  32. Ohkawa H, Ohishi N, Yagi K (1979) Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351–358CrossRefGoogle Scholar
  33. Parmar HS, Kar A (2007) Antidiabetic potential of Citrus sinensis and Punica granatum peel extracts in alloxan treated male mice. BioFactors 31:17–24CrossRefGoogle Scholar
  34. Perumal SR, Ponnampalam G (2007) current status of herbal and their future perspectives. Nature Preced 10101(1176):1Google Scholar
  35. Raj J, Chnadra M, Dogra TD, Pahuja M, Raina A (2013) Determination of median lethal dose of combination of Endosulfan and Cypermethrin in Wistar rat. Toxicol Int 20(1):1–5CrossRefGoogle Scholar
  36. Ramu S, Krishnaraj K, Devika A, Murali A (2014) Protective effects of Hippophae salicifolia D. Don fruit pulp extract in aluminium toxicity. Spatula DD 4(4):207–212CrossRefGoogle Scholar
  37. Rawat S, Kumar N, Kothiyal P (2013) Evaluate the anti-diabetic activity of Myrica esculenta in streptozotocin induced rats. Int J Univ Pharm Bio Sci 2(6):510–525Google Scholar
  38. Saikia M, Handique PJ (2013) Antioxidant and antibacterial activity of leaf, bark, pulp and seed extracts of seabuckthorn (Hippophae salicifolia D. Don) of Sikkim Himalayas. J Med Plants Res 7(19):1330–1338Google Scholar
  39. Shah NA, Khan MR (2014) Antidiabetic Effect of Sida cordata in alloxan induced diabetic rats. Biomed. Res. Int. 2014:671294PubMedPubMedCentralGoogle Scholar
  40. Sharma M, Siddique MW, Shamim AM, Gyanesh S, Pillai KK (2011) Evaluation of antidiabetic and antioxidant effects of seabuckthorn (Hippophae rhamnoides L.) in streptozotocin-nicotinamide induced diabetic rats. Open Conf Proc J 2:53–58CrossRefGoogle Scholar
  41. Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16:144–158Google Scholar
  42. Sun JE, Ao ZH, Lu ZM, Xu HY, Zhang XM, Dou WF, Xu ZH (2008) Antihyperglycemic and antilipidperoxidative e ffects of dry matter of culture broth of Inonotus obliquus in submerged culture on normal and alloxan-diabetes mice. J. Ethnopharmacol. 118:7–13CrossRefGoogle Scholar
  43. Usha T, Middha SK, Goyal AK, Karthik M, Manoj D, Faizan S, Goyal P, Prashanth HP, Pande V (2014) Molecular docking studies of anti-cancerous candidates in Hippophae rhamnoides and Hippophae salicifolia. J Biomed Res 28(5):406–415PubMedPubMedCentralGoogle Scholar
  44. Usha T, Middha SK, Narzary D, Brahma BK, Goyal AK (2017) In silico and in vivo based scientific evaluation of traditional anti-diabetic herb Hodgsonia heteroclita. Bangladesh J Pharmacol 12(2):165–166CrossRefGoogle Scholar
  45. Wang B, Lin L, Ni Q, Su CL (2011) Hippophae rhamnoides Linn. for treatment of diabetes mellitus: a review. J Med Plant Res 5(13):2599–2607Google Scholar
  46. Zharikova OL, Fokina VM, Nanovskaya TN, Hill RA, Mattison DR, Hankins GD, Ahmed MS (2009) Identification of the major human hepatic and placental enzymes responsible for the biotransformation of glyburide. Biochem. Pharmacol. 78(12):1483–1490CrossRefGoogle Scholar
  47. Zheng X, Long W, Liu G, Zhang X, Yang X (2012) Effect of seabuckthorn (Hippophae rhamnoides ssp. sinensis) leaf extract on the swimming endurance and exhaustive exercise-induced oxidative stress of rats. J. Sci. Food Agric. 92:736–742CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Sushil Kumar Middha
    • 1
  • Talambedu Usha
    • 2
  • Bharat Chandra Basistha
    • 3
  • Arvind Kumar Goyal
    • 4
    Email author
  1. 1.Department of BiotechnologyMaharani Lakshmi Ammanni College for WomenBengaluruIndia
  2. 2.Department of BiochemistryBangalore UniversityBengaluruIndia
  3. 3.Sikkim State Council of Science and TechnologyGangtokIndia
  4. 4.Centre for Bamboo Studies and Department of BiotechnologyBodoland UniversityKokrajharIndia

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