3 Biotech

, 9:308 | Cite as

Amelioration of antioxidant potential, toxicity, and antihyperglycemic activity of Hippophae salicifolia D. Don leaf extracts in alloxan-induced diabetic rats

  • Sushil Kumar Middha
  • Talambedu Usha
  • Bharat Chandra Basistha
  • Arvind Kumar GoyalEmail author
Original Article


Efficacy of several plant extracts in the clinical research for modulating oxidative stress correlated with diabetes mellitus (DM) is well documented. In the present study, we investigated the in vitro antioxidant activity, toxicity, and anti-diabetic activity of methanolic extract of Hippophae salicifolia leaves in normal and alloxan-induced diabetic wistar rats. H. salicifolia leaves were found to be rich in antioxidants. The acute toxicity test of methanolic extract of H. salicifolia leaves revealed that the median lethal dose (LD50) was found to be 3.92 g/kg body weight in mice. Administration of H. salicifolia leaves at 200 mg/kg and 400 mg/kg in alloxan-induced diabetic rats illustrated significant reduction (22% and 39%, respectively) in fasting blood glucose compared to diabetic control. Both the doses were found to be effective when compared to diabetic rats. The Hippophae-treated diabetic rats showed significant increase in the endogenous antioxidant enzymes, superoxide dismutase (50% and 74%, respectively), glutathione peroxidase (57% and 41%, respectively) and decrease in malondialdehyde (33% and 15%, respectively) levels. These results suggested that the methanolic leaf extract of H. salicifolia enhanced the antioxidant defence against reactive oxygen species produced under hyperglycaemic conditions.


Hippophae salicifolia Antioxidants Diabetes Toxicology Flavonoids Polyphenols Sea buckthorn 



Authors are grateful to Biotechnology-Skill Enhancement program, under Govt. of Karnataka at Maharani Lakshmi Ammanni College for Women, for funding animal house facility to carry out this study.

Author contribution

TU and AKG performed the experiments, analyzed the data and prepared the manuscript. BCB procured and prepared the sample and wrote a part of the manuscript. SKM conceived the idea, designed and coordinated the experiments, improved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in the studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Supplementary material

13205_2019_1840_MOESM1_ESM.jpg (42 kb)
DPPH scavenging activity of H. salicifolia compared to standard ascorbic acid (JPEG 41 kb)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Sushil Kumar Middha
    • 1
  • Talambedu Usha
    • 2
  • Bharat Chandra Basistha
    • 3
  • Arvind Kumar Goyal
    • 4
    Email author
  1. 1.Department of BiotechnologyMaharani Lakshmi Ammanni College for WomenBengaluruIndia
  2. 2.Department of BiochemistryBangalore UniversityBengaluruIndia
  3. 3.Sikkim State Council of Science and TechnologyGangtokIndia
  4. 4.Centre for Bamboo Studies and Department of BiotechnologyBodoland UniversityKokrajharIndia

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