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3 Biotech

, 9:301 | Cite as

Transcriptomic profiles of human HaCaT cells in response to angelica polysaccharide

  • Chunshui YuEmail author
  • Linli Liu
  • Qian Liao
  • Hanwen Wang
  • Fei Ouyang
  • Hui Fang
  • Yan Wan
  • Qinlian Lu
  • Tingting Liu
Original Article

Abstract

The aim of this study was to explore the anti-psoriatic effect and potential mechanism of Angelica polysaccharide (APS) on an in vitro HaCaT cell model. MTS assay was performed to determine whether APS has the ability to inhibit the proliferation of HaCaT cells. RNA-sequencing (RNA-seq) was performed to investigate the underlying mechanism of APS. Quantitative real-time PCR (qRT-PCR) was used to verify the accuracy of RNA-seq data. Our MTS assay results demonstrated that APS time- and concentration-dependently inhibits the proliferation of HaCaT cells. The anti-proliferation property of APS suggests that APS may have anti-psoriatic effect. In the RNA-seq part, comparison between the CK group (i.e., Control group) and ASP groups revealed dramatic differences [468 differentially expressed genes (DEGs) for CK group vs. ASP50 group; 563 DEGs for CK group vs. ASP100 group; 532 DEGs for CK group vs. ASP200 group]. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrich analysis performed on all DEGs failed to find any significant enriched GO terms or KEGG pathways to explain the anti-proliferative effect of APS. All DEGs were then classified into 20 expression profiles by trend analysis. Interestingly, cell proliferation-related GO terms were mostly dispersed in the profile 2 and 17. DEGs enriched in these terms were then analyzed. After literature retrieval, DEGs such as SERPINE1, SMAD6, CTGF, and TGF-β were suspected to closely relevant to the anti-proliferation effect of APS. qRT-PCR results showed similar expression trend with RNA-seq data for 8 out of 10 genes, indicating our sequence data are reliable.

Keywords

Angelica polysaccharide Psoriasis Transcriptome 

Notes

Acknowledgements

This work was supported by grants from the Project of Sichuan Science and Technology Bureau (2016JY0214), the Scientific Research Project of Sichuan Health and Family Planning Commission (150243) and the Scientific Research Project of Suining Municipality (2015S23).

Compliance with ethical standards

Conflict of interest

The author declares that they have no competing interests.

Supplementary material

13205_2019_1828_MOESM1_ESM.xlsx (183 kb)
Supplement table S1. All DEGs after APS treatment as compared with the CK group. Genes with log2(FC)>1 and FDR<0.05 were up-regulated; Genes with log2(FC) <-1 and FDR<0.05 were down-regulated. (XLSX 182 kb)
13205_2019_1828_MOESM2_ESM.xlsx (13 kb)
Supplement table S2. Significantly enriched GO terms for all DEGs (XLSX 13 kb)
13205_2019_1828_MOESM3_ESM.xlsx (21 kb)
Supplement table S3.Significantly enriched GO terms for DEGs in all profiles (XLSX 21 kb)
13205_2019_1828_MOESM4_ESM.xlsx (14 kb)
Supplement table S4. Genes relevant to cell proliferation (XLSX 13 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Chunshui Yu
    • 1
    Email author
  • Linli Liu
    • 1
  • Qian Liao
    • 1
  • Hanwen Wang
    • 1
  • Fei Ouyang
    • 1
  • Hui Fang
    • 1
  • Yan Wan
    • 1
  • Qinlian Lu
    • 1
  • Tingting Liu
    • 1
  1. 1.Department of DermatologySuining Central HospitalSuiningChina

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