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3 Biotech

, 9:299 | Cite as

WA-CMS-based iso-cytoplasmic restorers derived from commercial rice hybrids reveal distinct population structure and genetic divergence towards restorer diversification

  • Amit Kumar
  • Vikram Jeet Singh
  • S. Gopala Krishnan
  • K. K. Vinod
  • Prolay Kumar Bhowmick
  • M. Nagarajan
  • Ranjith Kumar Ellur
  • Haritha Bollinedi
  • Ashok Kumar SinghEmail author
Original Article

Abstract

One hundred diverse iso-cytoplasmic restorer (ICR) lines carrying WA cytoplasm indicated significant but moderate variability for agro-morphological traits as well as for the microsatellite-based allele patterns. There were two major groups of ICRs based on agro-morphological clustering. Simple sequence repeat (SSR) markers identified allelic variants with an average of 2.48 alleles per locus and the gene diversity (GD) ranged from 0.02 to 0.62 at different loci. ICR lines showed a genetic structure involving two sub-populations, POP1 and POP2. Both the subpopulations had the presence of admixture lines. Nearest ancestry-based grouping of ICRs by neighbour-joining (NJ) method showed near similar grouping as that of sub-population division. The POP2 was the largest group but with fewer admixed lines. POP1 was more distinct than POP2. Since the hybrid parents of the ICRs had limited diversity on maternal lineage, paternal lineage was concluded as the major contributor to the observed divergence and population differentiation. ICRs developed from certain hybrids were more genetically distinct than other hybrids. Even with the moderate variability, ICRs could be considered as a potential source of fertility restoration in hybrid development because of their distinct population structure and the full complement of restorer genes they contained. ICR lines with high per se performance can be utilized in hybrid rice development by estimating their combining ability.

Keywords

Iso-cytoplasmic restorers Combining ability Population structure SSR markers Hybrid rice 

Notes

Acknowledgements

The study is part of the PhD research of the first author. The first author acknowledges the Post Graduate School, ICAR–IARI, New Delhi for providing the necessary facilities for the research study. The authors gratefully acknowledge the funding assistance from the Indian Council of Agricultural Research under Consortia Research Platform on Hybrid Technology (Project Code # 12-142). Technical help rendered by Binder Singh, Devinder Singh, Mahendran and Bibekananda Ray in maintaining the crop is thankfully acknowledged.

Author contributions

Conceptualization of research (AKS, GKS); Designing of the experiments (AKS, GKS, AK); Contribution of experimental materials (AK, PKB); Execution of field/lab experiments and data collection (AK, GKS, VJS, PKB, MN); Analysis of data and interpretation (AK, GKS, KKV, PKB, AKS); Preparation of manuscript (AK, GKS, KKV, PKB, AKS, RKE, HB).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13205_2019_1824_MOESM1_ESM.pdf (524 kb)
Supplementary material 1 (PDF 523 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Amit Kumar
    • 1
    • 2
  • Vikram Jeet Singh
    • 1
  • S. Gopala Krishnan
    • 1
  • K. K. Vinod
    • 3
  • Prolay Kumar Bhowmick
    • 1
  • M. Nagarajan
    • 3
  • Ranjith Kumar Ellur
    • 1
  • Haritha Bollinedi
    • 1
  • Ashok Kumar Singh
    • 1
    Email author
  1. 1.Division of GeneticsICAR-Indian Agricultural Research Institute (ICAR-IARI)New DelhiIndia
  2. 2.Plant Breeding, ICAR-Research Complex for North Eastern Hill RegionUmiamIndia
  3. 3.Rice Breeding and Genetics Research CentreICAR-IARIAduthuraiIndia

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