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3 Biotech

, 9:263 | Cite as

Functional characterization of LkERF-B2 for improved salt tolerance ability in Arabidopsis thaliana

  • Beibei Cao
  • Lixiang Shu
  • Ai LiEmail author
Original Article

Abstract

The ethylene response factors have been reported to play critical roles in developmental and environmental responses in plants. In the present study, an ERF transcription factor gene was aimed to be identified from Larix kaempferi. Molecular characteristics and function of this gene were further explored. The result showed that a 1344 bp ERF transcription factor gene containing initiation and termination codon was obtained by RT-PCR and named LkERF-B2. LkERF-B2 gene encoded 447 amino acids containing a typical AP2/ERF domain. Alignment of predicted amino acid sequence of LkERF-B2 in various plant species showed that this ERF transcription factor was highly homologous (79.0%) with that of Picea sitchensi. To elucidate the function of LkERF-B2, LkERF-B2 overexpression vector was successfully constructed and transformed to Arabidopsis thaliana via dip flower. Compared with control plant, LkERF-B2 overexpressed transgenic A. thaliana showed a significantly higher survival rate under cold, heat, NaCl and drought stresses. NaCl stress analysis revealed that control and transgenic Arabidopsis were both flowering earlier under 100 and 150 mM/L NaCl treatment. While under 200–300 mM/L NaCl treatment, the growth of control plant was significantly inhibited compared with transgenic A. thaliana. Salt injury rate and salt injury index of transgenic Arabidopsis were lower than those of the control. Further investigation showed that transgenic Arabidopsis exhibited much higher content of chloroplast pigments under different NaCl concentration. Meanwhile, the activity of SOD and POD was also enhanced in transgenic A. thaliana. These results suggested that LkERF-B2 was a key transcription factor and could lead to enhanced salt stress tolerance.

Keywords

Larix kaempferi LkERF-B2 NaCl tolerance Functional characterization 

Abbreviations

GUS

β-Glucuronidase enzyme

MS

Murashige and Skoog

NBT

Nitroblue tetrazolium

SOD

Superoxide dismutase

POD

Peroxidase

ROS

Reactive oxygen species

Notes

Acknowledgements

This work was supported by Tianjin Agricultural University Graduate Training Quality Improvement Project (No. 101018), National Natural Science Foundation (No. 31300564, No. 31800572), Tianjin “131” Innovative Talents Training Project, Modern Industrial System Fruit Tree Physiological and Ecological Post (ITTFPRS2018002).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.College of Horticulture and Landscape Architecture (Key Laboratory of Fruit Science)Tianjin Agricultural UniversityTianjinChina

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