Analysis of allelic variation in wild potato (Solanum) species by simple sequence repeat (SSR) markers
- 32 Downloads
Allelic variation in wild potato (Solanum) species was analysed using 14 simple sequence repeat (SSR) markers. SSR allelic profiles showed high polymorphism and distinctness among the wild species. A total of 109 alleles of 14 polymorphic SSR markers were scored in 82 accessions belonging to 22 wild potato species. Allele size ranged from a minimum of 104 bp (STI0030) to a maximum of 304 bp (STM5114). Number of SSR alleles per marker ranged from 4 (STM5127/STM1053) to 13 (STM0019), whereas PIC value varied between 0.66 (STM1053) and 0.91 (STM0019). Cluster analysis using SSR allelic profiles of 82 accessions grouped showed 5 major clusters (I–V) based on the Dice similarity coefficient using neighbour-joining clustering method. Distinct allelic variations were observed among the accessions irrespective of the origin country, series and species. Our study suggests that SSR-based molecular characterization of wild potato species is accession specific and development of an allelic dataset for all the accessions would strengthen their utilization in potato research in future.
KeywordsAlleles Diversity Potato SSR marker Solanum species
The authors thank the Competent Authority, ICAR-CPRI, Shimla; Germplasm and Biotechnology programmes (HORTCPRICIL201500300131); and CABin scheme (IASRI, New Delhi) for necessary support. Special thanks to Mr. Sheeshram Thakur for in vitro maintenance of wild species, and Mr. CM Bist for SSR analysis. The International GenBank are gratefully acknowledged for providing the TPS of the wild species.
JKT designed the experiment. JKT, NL, SD and RZ performed the SSR analysis. VK provided the samples. JKT analysed the data and wrote the manuscript, and SKC edited it. All authors read and confirmed the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
- Gopal J, Kumar V, Sandhu SK (2003) Biosystematics and genetic resources of potato. In: Khurana SMP, Minhas JS, Pandey SK (eds) The potato production and utilization in subtropics. Mehta Publishers, New Delhi, pp 31–47Google Scholar
- Hawkes JG (1990) The potato: evolution, biodiversity and genetic resources. Belhaven Press, Washington, DCGoogle Scholar
- Liao H, Guo H (2014) Using SSR to evaluate the genetic diversity of potato cultivars from Yunnan province (SW China). Acta Biol Cracov Ser Bot 56(1):16–27Google Scholar
- Perrier X, Jacquemoud-Collet JP (2006) DARwin software. http://darwin.cirad.fr/darwin. Accessed 30 Apr 2019
- Rohlf F (2006) NTSYSpc: numerical taxonomy system (ver. 2.2). Exeter Publishing, Ltd., SetauketGoogle Scholar
- Tiwari JK, Singh BP, Gopal J, Poonam Patil VU (2013) Molecular characterization of the Indian Andigena potato core collection using microsatellite markers. Afr J Biotechnol 12:1025–1033Google Scholar