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3 Biotech

, 9:262 | Cite as

Analysis of allelic variation in wild potato (Solanum) species by simple sequence repeat (SSR) markers

  • Jagesh Kumar TiwariEmail author
  • Nilofer Ali
  • Sapna Devi
  • Rasna Zinta
  • Vinod Kumar
  • Swarup Kumar Chakrabarti
Original Article
  • 32 Downloads

Abstract

Allelic variation in wild potato (Solanum) species was analysed using 14 simple sequence repeat (SSR) markers. SSR allelic profiles showed high polymorphism and distinctness among the wild species. A total of 109 alleles of 14 polymorphic SSR markers were scored in 82 accessions belonging to 22 wild potato species. Allele size ranged from a minimum of 104 bp (STI0030) to a maximum of 304 bp (STM5114). Number of SSR alleles per marker ranged from 4 (STM5127/STM1053) to 13 (STM0019), whereas PIC value varied between 0.66 (STM1053) and 0.91 (STM0019). Cluster analysis using SSR allelic profiles of 82 accessions grouped showed 5 major clusters (I–V) based on the Dice similarity coefficient using neighbour-joining clustering method. Distinct allelic variations were observed among the accessions irrespective of the origin country, series and species. Our study suggests that SSR-based molecular characterization of wild potato species is accession specific and development of an allelic dataset for all the accessions would strengthen their utilization in potato research in future.

Keywords

Alleles Diversity Potato SSR marker Solanum species 

Notes

Acknowledgements

The authors thank the Competent Authority, ICAR-CPRI, Shimla; Germplasm and Biotechnology programmes (HORTCPRICIL201500300131); and CABin scheme (IASRI, New Delhi) for necessary support. Special thanks to Mr. Sheeshram Thakur for in vitro maintenance of wild species, and Mr. CM Bist for SSR analysis. The International GenBank are gratefully acknowledged for providing the TPS of the wild species.

Author contributions

JKT designed the experiment. JKT, NL, SD and RZ performed the SSR analysis. VK provided the samples. JKT analysed the data and wrote the manuscript, and SKC edited it. All authors read and confirmed the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

13205_2019_1785_MOESM1_ESM.doc (236 kb)
Supplementary material 1 (DOC 236 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Jagesh Kumar Tiwari
    • 1
    Email author
  • Nilofer Ali
    • 1
  • Sapna Devi
    • 1
  • Rasna Zinta
    • 1
  • Vinod Kumar
    • 1
  • Swarup Kumar Chakrabarti
    • 1
  1. 1.ICAR-Central Potato Research InstituteShimlaIndia

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