3 Biotech

, 9:212 | Cite as

Synthesis of gold and silver nanoparticles using flavonoid quercetin and their effects on lipopolysaccharide induced inflammatory response in microglial cells

  • Zeliha Duygu Ozdal
  • Ertugrul SahmetliogluEmail author
  • Ibrahim Narin
  • Ahmet CumaogluEmail author
Original Article


Quercetin is a plant origin phytochemical with several pharmaceutical activities such as antioxidant, immunomodulatory, and anti-inflammatory effects. However, consumption of quercetin is limited due to its low aqueous solubility and poor bioavailability. The aim of the present study was to synthesize silver and gold nanoparticles of quercetin with a view to improve its aqueous phase solubility and investigate the effects on LPS-induced neuroinflammation in BV-2 microglial cells. The average size of silver and gold-quercetin nanoparticles was 53 and 27 nm, respectively. Absorption peaks in the UV–Vis spectra were observed at 555 and 405 nm for gold and silver-quercetin nanoparticles, respectively. The particle size and mapping of silver and gold-quercetin nanoparticles were also determined using a STEM detector. The inflammatory stimulation of the BV-2 cells with LPS caused an elevated release of proinflammatory prostaglandin, E2, nitric oxide (NO), upregulated cyclooxygenase-2, inducible NO synthase mRNA, and protein levels, which were markedly inhibited by the pretreatment with gold-quercetin nanoparticles (highly soluble in water) without causing any cytotoxic effects. The findings of the present study suggest that the potential of gold-quercetin nanoparticles are much better than quercetin and that gold-quercetin nanoparticles might provide protection against inflammatory neurodegenerative disease via suppression of acute microglial activation.


Quercetin Microglia Inflammation Nanoparticle 





Inducible nitric oxide synthase


Prostaglandin E2


Nitric oxide




Energy-dispersive X-ray


Scanning electron microscopy


Dimethyl sulfoxide


Dulbecco’s modified Eagle medium


Fetal bovine serum


Polyacrylamide gel electrophoresis


Bicinchoninic acid assay


Glyceraldehyde 3-phosphate dehydrogenase


Mitogen-activated protein kinases



Thanks to Dr. Lucia Rackova (from the Slovak Academy of Sciences) for kindly supplying BV-2 cells. This research was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) 2209A-Undergraduate Scholarship Program For National Students (1919B011501082).

Compliance with ethical standards

Conflict of interest

There is no conflict of interest to declare.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Faculty of PharmacyErciyes UniversityKayseriTurkey
  2. 2.Nanotechnology Research CenterErciyes UniversityKayseriTurkey
  3. 3.Safiye Çıkrıkçıoğlu Vocational SchoolKayseri UniversityKayseriTurkey
  4. 4.Department of Analytical Chemistry, Faculty of PharmacyErciyes UniversityKayseriTurkey
  5. 5.Department of Biochemistry, Faculty of PharmacyErciyes UniversityKayseriTurkey

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