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3 Biotech

, 9:170 | Cite as

A rapid and sensitive enzymatic assay for 2,3-butanediol

  • Gyu Bi Lee
  • Yun Jae KimEmail author
  • Jae Kyu Lim
  • Tae Wan Kim
  • Sung Gyun Kang
  • Jung-Hyun Lee
  • Hyun Sook LeeEmail author
Short Reports
  • 31 Downloads

Abstract

In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP+ using Clostridium ljungdahlii 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0.01 mM of 2,3-BDO, while the high-performance liquid chromatography (HPLC) method required a much higher concentration than 0.15 mM. Gratifyingly, the developed method was 15 times more sensitive than the HPLC method. When the enzymatic assay was applied to high-throughput screening, the enzymatic assay detected 14 positive samples out of 23 tested, as compared to 8 by the HPLC method. These results suggest that the enzymatic assay is an effective screening method for the detection of 2,3-BDO-producing microbes in a microtiter plate-based format.

Keywords

2,3-Butanediol Enzymatic assay 2,3-Butanediol dehydrogenase High-throughput screening 

Notes

Acknowledgements

This work was supported by the KIOST In-house Program (PE99722) and the understanding the deep-sea biosphere on seafloor hydrothermal vents in the Indian Ridge Program (20170411) of the Ministry of Ocean and Fisheries of the Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Supplementary material

13205_2019_1705_MOESM1_ESM.docx (57 kb)
Supplementary material 1 (DOCX 56 kb)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Korea Institute of Ocean Science and TechnologyBusanRepublic of Korea
  2. 2.Department of Marine BiotechnologyUniversity of Science and TechnologyDaejeonRepublic of Korea
  3. 3.Department of Biotechnology and BioengineeringChonnam National UniversityGwangjuRepublic of Korea

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