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3 Biotech

, 9:162 | Cite as

Complete genome sequence of a marine-sediment-derived bacterial strain Bacillus velezensis SH-B74, a cyclic lipopeptides producer and a biopesticide

  • Zongwang MaEmail author
  • Jiangchun HuEmail author
Genome Reports

Abstract

A marine-sediment sample-derived strain Bacillus velezensis SH-B74 has the capacity to produce cyclic lipopeptides (CLPs), and these CLPs secreted by the strain show biological activities against various pests under both in vitro and in planta conditions, such evidence has supported that the strain SH-B74 is a biopesticide. To get a better insight into the mechanisms on the control of the pesticides by the strain, a genome sequencing project has been applied to the genomic DNA of the strain SH-B74. The results show that the strain SH-B74 has a chromosome size of 4,042,190 bp, with a GC content of 46.5%, in addition, the strain contains a 61,634 bp plasmid pSH-B74, with a GC content of 40.8%. Data from bioinformatic analysis reveal that the strain SH-B74 has genes with the capacity to increase environmental adaptation, promote the rhizosphere fitnesses and secrete a spectrum of antibiotics, including nonribosomal peptide synthetases (NRPSs)-derived CLPs bacillopeptin, plipastatin, and surfactin. The presence of CLPs in the bacterial cultures of the strain SH-B74 was confirmed further by LC–MS analysis. Thus, genome sequencing and analyses together with chemical analysis reveal the promising perspectives of the strain SH-B74 that are of spectacular importance to its trait as a plant beneficial microbe to be used in agriculture practices.

Keywords

Bacillus velezensis Marine-sediment-derived bacterium Full genome sequence Cyclic lipopeptides Environmental adaptation and fitness 

Notes

Acknowledgements

Z. Ma conceived and designed the study, conducted the experiments and wrote the paper. This work was supported by a grant from Science and Technology Service Network Initiative of Chinese Academy of Sciences (CAS) (Grant No. KFJ-EW-STS-143) to J. Hu.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.College of Life ScienceNorthwest Normal UniversityLanzhouChina
  2. 2.Institute of Applied EcologyChinese Academy of SciencesShenyangChina

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