3 Biotech

, 9:150 | Cite as

Effect of light/dark cycle on nitrate and phosphate removal from synthetic wastewater based on BG11 medium by Scenedesmus sp.

  • Adnan Habibi
  • Ghorban Ali Nematzadeh
  • Farshid Pajoum shariati
  • Hossein Delavari AmreiEmail author
  • Abolghasem Teymouri
Original Article


In this study, microalgae growth in the synthetic wastewater and their ability to remove nutrients under different light levels was investigated. For this purpose, a comparative study was conducted on freshwater microalgae Scenedesmus sp. to evaluate their performance to remove nitrate and phosphate from both slaughterhouse and dairy synthetic treated wastewaters, under different light/dark cycles (12/12, 16/8 and 24/0 h), in Erlenmeyer flasks. The best light/dark cycles in Erlenmeyer flasks for nitrate and phosphate removal and growth were obtained at 24/0 h. Moreover, nitrate and phosphate removal under light conditions at 24/0 h light/dark cycles were tested in a designed open raceway pond. The maximum nitrate removal in slaughterhouse and dairy synthetic wastewater was 78% and 99.7%, and the phosphate removal was 31% and 68%, respectively. Furthermore, the highest biomass productivity in dairy and slaughterhouse synthetic wastewater during 9 days was 0.65 g L−1 and 1.5 g L−1, respectively. Thus, Scenedesmus sp. could be potential candidates by showing their intrinsic merit, for the reduction of nitrate and phosphate residue levels from dairy and slaughterhouse synthetic wastewaters in open raceway ponds.


Scenedesmus sp. Microalgae Synthetic wastewater Nutrient removal 



Dairy synthetic wastewater


Slaughterhouse synthetic wastewater

12/12 DSW

12/12 light/dark cycle dairy synthetic wastewater

16/8 DSW

16/8 light/dark cycle dairy synthetic wastewater

24/0 DSW

24/0 light/dark cycle dairy synthetic wastewater

12/12 SSW

12/12 light/dark cycle slaughterhouse synthetic wastewater

16/8 SSW

16/8 light/dark cycle slaughterhouse synthetic wastewater

24/0 SSW

24/0 light/dark cycle slaughterhouse synthetic wastewater



The valuable collaboration of faculty authorities of Institute of Genetics and Biotechnology Tabarestan, University of Agricultural Sciences and Natural Resources (Sari, Iran) to provide necessary facilities for conduction of this study is highly appreciated. Also a special thanks to Reza khalili for cooperation in this project.

Compliance with ethical standards

Conflict of interest

The author(s) declared no potential conflicts of interest with respect to research, authorship, and/or publication of this article.


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Chemical Engineering, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources UniversitySariIran
  3. 3.Department of Chemical Engineering, Faculty of EngineeringUniversity of BojnordBojnordIran

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