3 Biotech

, 9:137 | Cite as

Morpho-taxonomic, genetic, and biochemical characterization of freshwater microalgae as potential biodiesel feedstock

  • Anisha Sehgal
  • Kongkana Goswami
  • Mintu Pal
  • Channakeshavaiah Chikkaputtaiah
  • Pankaj Chetia
  • Hari Prasanna Deka BoruahEmail author
Original Article


In the present study, seven axenic fresh water microchlorophytes were isolated and identified as Tetradesmus dimorphus (NEIST BT-1), Chlorella sorokiniana (NEIST BT-2), Desmodesmus sp. (NEIST BT-10), Selenastrum sp. (NEIST BT-A6), Tetradesmus obliquus (NEIST BT-A1), Tetradesmus sp. (NEIST BT-A10), and Asterarcys sp. (NEIST BT-A15) based on morphological and molecular characterization. Their potential to be used as biodiesel feedstock was evaluated depending on their growth characteristics and lipid profiles. Among the seven isolates, NEIST BT-2 was found to be the most promising candidate owing to its high biomass yield (2.09 ± 0.037 g L−1) and lipid productivity (107.60 ± 10.175 mg L−1 day−1). The gas chromatography analysis confirmed the presence of significant amounts of palmitic acid, linoleic acid, linolenic acid, and oleic acid in the isolate which are some of the major constituents of any biodiesel. The predictive models showed that the biodiesel from this isolate has ideal fuel properties which comply with the ASTM D6751 and EN 14214 specifications. These findings demonstrate that NEIST BT-2 can be used as a prospective candidate for consideration of large-scale biodiesel production.


Biodiesel properties Confocal microscopy Fatty acid profiling Lipid Phylogeny 



American society for testing and materials


Biomass productivity


Cold filter plugging point


Cetane number


Cloud point


Dry cell mass


Dimethyl sulfoxide


Degree of unsaturation


European Standards


Fatty acid methyl ester


Flame ionization detector


Gross calorific value


Iodine value


Lipid content


Long-chain saturated factor


Lipid productivity


Monounsaturated fatty acid


Net calorific value


Optical density


Polyunsaturated fatty acid


Scanning electron microscope


Saturated fatty acid


Saponification value



This work was supported with funds from Council of Scientific & Industrial Research, New Delhi in the form of research grants from BioEn (CSC-0116, FTT-2001). Technical support was received from Dr. B. L. A Prabhavathi Devi, Senior Principal Scientist, CSIR-Indian Institute of Chemical Technology, Hyderabad for performing the fatty acid profiling of the strain using gas chromatography.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13205_2019_1664_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 KB)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Biotechnology Group, Biological Science and Technology DivisionCSIR-North East Institute of Science and TechnologyJorhatIndia
  2. 2.Department of Life SciencesDibrugarh UniversityDibrugarhIndia
  3. 3.Academy of Scientific and Innovative ResearchNew DelhiIndia

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