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3 Biotech

, 9:137 | Cite as

Morpho-taxonomic, genetic, and biochemical characterization of freshwater microalgae as potential biodiesel feedstock

  • Anisha Sehgal
  • Kongkana Goswami
  • Mintu Pal
  • Channakeshavaiah Chikkaputtaiah
  • Pankaj Chetia
  • Hari Prasanna Deka BoruahEmail author
Original Article
  • 43 Downloads

Abstract

In the present study, seven axenic fresh water microchlorophytes were isolated and identified as Tetradesmus dimorphus (NEIST BT-1), Chlorella sorokiniana (NEIST BT-2), Desmodesmus sp. (NEIST BT-10), Selenastrum sp. (NEIST BT-A6), Tetradesmus obliquus (NEIST BT-A1), Tetradesmus sp. (NEIST BT-A10), and Asterarcys sp. (NEIST BT-A15) based on morphological and molecular characterization. Their potential to be used as biodiesel feedstock was evaluated depending on their growth characteristics and lipid profiles. Among the seven isolates, NEIST BT-2 was found to be the most promising candidate owing to its high biomass yield (2.09 ± 0.037 g L−1) and lipid productivity (107.60 ± 10.175 mg L−1 day−1). The gas chromatography analysis confirmed the presence of significant amounts of palmitic acid, linoleic acid, linolenic acid, and oleic acid in the isolate which are some of the major constituents of any biodiesel. The predictive models showed that the biodiesel from this isolate has ideal fuel properties which comply with the ASTM D6751 and EN 14214 specifications. These findings demonstrate that NEIST BT-2 can be used as a prospective candidate for consideration of large-scale biodiesel production.

Keywords

Biodiesel properties Confocal microscopy Fatty acid profiling Lipid Phylogeny 

Abbreviations

ASTM

American society for testing and materials

BP

Biomass productivity

CFPP

Cold filter plugging point

CN

Cetane number

CP

Cloud point

DCM

Dry cell mass

DMSO

Dimethyl sulfoxide

DU

Degree of unsaturation

EN

European Standards

FAME

Fatty acid methyl ester

FID

Flame ionization detector

GCV

Gross calorific value

IV

Iodine value

LC

Lipid content

LCSF

Long-chain saturated factor

LP

Lipid productivity

MUFA

Monounsaturated fatty acid

NCV

Net calorific value

OD

Optical density

PUFA

Polyunsaturated fatty acid

SEM

Scanning electron microscope

SFA

Saturated fatty acid

SV

Saponification value

Notes

Acknowledgements

This work was supported with funds from Council of Scientific & Industrial Research, New Delhi in the form of research grants from BioEn (CSC-0116, FTT-2001). Technical support was received from Dr. B. L. A Prabhavathi Devi, Senior Principal Scientist, CSIR-Indian Institute of Chemical Technology, Hyderabad for performing the fatty acid profiling of the strain using gas chromatography.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13205_2019_1664_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 KB)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Biotechnology Group, Biological Science and Technology DivisionCSIR-North East Institute of Science and TechnologyJorhatIndia
  2. 2.Department of Life SciencesDibrugarh UniversityDibrugarhIndia
  3. 3.Academy of Scientific and Innovative ResearchNew DelhiIndia

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