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3 Biotech

, 9:138 | Cite as

Distribution of antibiotic resistance and virulence factors among the bacteria isolated from diseased Etroplus suratensis

  • Aswani Ravi
  • Soumya Das
  • Jasim Basheer
  • Aswathy Chandran
  • Chinnu Benny
  • Sindhura Somaraj
  • Sebastian Korattiparambil Sebastian
  • Jyothis Mathew
  • Radhakrishnan Edayileveettil KrishnankuttyEmail author
Original Article
  • 33 Downloads

Abstract

Considering the emerging concern with the antimicrobial resistance (AMR) evolution, the study has been designed to identify the antibiotic resistance and virulence properties of culturable bacteria isolated from the diseased fish Etroplus suratensis. This has resulted in the purification of 18 morphologically distinct bacterial isolates which were identified by both biochemical and molecular methods. Antibiotic resistance analysis showed the resistance of these isolates to multiple antibiotics and remarkable evolution of AMR. Further screening for virulence factors confirmed five isolates to be positive for haemolytic activity, eight with caseinase, four with DNase, one with gelatinase and three with biofilm-forming properties. In addition to these, the isolates were subjected to PCR-based screening to detect the presence of genes coding for aerolysin and haemolysin. Results showed the presence aerolysin gene in the isolates ESS3.2, ESS3.8, ESI3.3 and ESS3.6, while haemolysin gene was observed to be present in ESG3.1 and ESI3.2. The observed results hence indicate the need for frequent monitoring of these properties among bacterial isolates from diverse environment especially those associated with edible fish.

Keywords

Etroplus suratensis Aquaculture Antimicrobial resistance Virulence mechanisms 

Notes

Compliance with ethical standards

Conflict of interest

The authors declared that they have no conflict of interest.

Supplementary material

13205_2019_1654_MOESM1_ESM.docx (114 kb)
Supplementary material 1 (DOCX 114 KB)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Aswani Ravi
    • 1
  • Soumya Das
    • 1
  • Jasim Basheer
    • 1
  • Aswathy Chandran
    • 2
  • Chinnu Benny
    • 2
  • Sindhura Somaraj
    • 2
  • Sebastian Korattiparambil Sebastian
    • 3
  • Jyothis Mathew
    • 1
  • Radhakrishnan Edayileveettil Krishnankutty
    • 1
    Email author
  1. 1.School of BiosciencesMahatma Gandhi UniversityKottayamIndia
  2. 2.Department of Biotechnology and Applied MicrobiologySt. Thomas CollegeKottayamIndia
  3. 3.Department of ZoologyGovernment CollegeKottayamIndia

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