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3 Biotech

, 9:116 | Cite as

Complete genome sequencing of Lactobacillus plantarum CAUH2 reveals a novel plasmid pCAUH203 associated with oxidative stress tolerance

  • Zhengyuan Zhai
  • Yang Yang
  • Jiaojiao Wang
  • Guohong Wang
  • Fazheng Ren
  • Yanling HaoEmail author
Short Reports
  • 59 Downloads

Abstract

Lactobacillus plantarum is remarkably adaptable to diverse habitats and is widely used in food industry. In this study, the genome sequence of L. plantarum CAUH2 was analyzed and compared with other L. plantarum genome sequences. A comparison of the genome sequence of CAUH2 to L. plantarum ST-III reveals that the similarity of these two genomes reached up to 99% identity with 98% coverage, but the plasmid profiles of CAUH2 and ST-III are different. Notably, plasmid pCAUH203 in L. plantarum CAUH2 harbors seven genes involved in oxidative stress response, such as genes encoding thioredoxin-disulfide reductase, thioredoxin and DNA protection protein. Due to plasmid pCAUH203, the thioredoxin reductase activity of CAUH2 was 2.1-fold higher than that of ST-III. When exposed to 5 mM H2O2, this activity was further increased to 9.87 ± 1.60 mU per mg protein in CAUH2, which was 2.7-fold higher than that of ST-III, indicating that thioredoxin antioxidant system encoded by pCAUH203 might contribute to the H2O2 resistance. This hypothesis was further confirmed by survival assay under 10 mM H2O2 stress. The survival rate of CAUH2 was 12-fold higher than that of ST-III. Therefore, the complete genome sequencing of L. plantarum CAUH2 provides new insights into the molecular mechanism of its oxidative stress resistance.

Keywords

L. plantarum CAUH2 Complete genome sequence Plasmid H2O2 resistance Thioredoxin reductase 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (contract No.31701571).

Author contributions

ZZ, FR and YH designed research; ZZ, YY, JW and GW performed research; YY and GW contributed new reagents or analytic tools; ZZ, FR and YH analyzed the data and wrote the paper. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13205_2019_1653_MOESM1_ESM.docx (39 kb)
Supplementary material 1 (DOCX 38 KB)

References

  1. Arnér ES, Holmgren A (2000) Physiological functions of thioredoxin and thioredoxin reductase. Eur J Biochem 267:6102–6109CrossRefGoogle Scholar
  2. Arnér ES, Holmgren A (2001) Measurement of thioredoxin and thioredoxin reductase. Curr Protoc Toxicol, Chap. 7:Unit 7.4.Google Scholar
  3. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O (2008) The RAST Server: rapid annotations using subsystems technology. BMC genomics 9:75CrossRefGoogle Scholar
  4. Chen C, Ai L, Zhou F, Ren J, Sun K, Zhang H, Chen W, Guo B (2012) Complete nucleotide sequence of plasmid pST-III from Lactobacillus plantarum ST-III. Plasmid 67:236–244CrossRefGoogle Scholar
  5. Corcoran BM, Stanton C, Fitzgerald G, Ross RP (2008) Life under stress: the probiotic stress response and how it may be manipulated. Curr Pharm Des 14:1382–1399CrossRefGoogle Scholar
  6. Darling AC, Mau B, Blattner FR, Perna NT (2004) Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 14:1394–1403CrossRefGoogle Scholar
  7. Grohmann E, Muth G, Espinosa M (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev 67:277–301CrossRefGoogle Scholar
  8. Ito Y, Kawai Y, Arakawa K, Honme Y, Sasaki T, Saito T (2009) Conjugative plasmid from Lactobacillus gasseri LA39 that carries genes for production of and immunity to the circular bacteriocin gassericin A. Appl Environ Microbiol 75:6340–6351CrossRefGoogle Scholar
  9. Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, Jones SJ, Marra MA (2009) Circos: an information aesthetic for comparative genomics. Genome Res 19:1639–1645CrossRefGoogle Scholar
  10. Leroy F, De Vuyst L (2004) Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci Tech 15:67–78CrossRefGoogle Scholar
  11. Li R, Zhai Z, Yin S, Huang Y, Wang Q, Luo Y, Hao Y (2009) Characterization of a rolling-circle replication plasmid pLR1 from Lactobacillus plantarum LR1. Curr Microbiol 58:106–111CrossRefGoogle Scholar
  12. Lu J, Holmgren A (2014) The thioredoxin antioxidant system. Free Radic Biol Med 66:75–87CrossRefGoogle Scholar
  13. Martino ME, Bayjanov JR, Caffrey BE, Wels M, Joncour P, Hughes S, Gillet B, Kleerebezem M, van Hijum SA, Leulier F (2016) Nomadic lifestyle of Lactobacillus plantarum revealed by comparative genomics of 54 strains isolated from different habitats. Environ Microbiol 18:4974–4989CrossRefGoogle Scholar
  14. Mills S, Stanton C, Fitzgerald GF, Ross RP (2011) Enhancing the stress responses of probiotics for a lifestyle from gut to product and back again. Microb Cell Fact 10(Suppl 1):S19CrossRefGoogle Scholar
  15. Nieuwboer M, Hemert S, Claassen E, Vos WM (2016) Lactobacillus plantarum WCFS1 and its host interaction: a dozen years after the genome. Microb Biotechnol 9:452–465CrossRefGoogle Scholar
  16. O’Sullivan DJ, Klaenhammer TR (1993) Rapid mini-prep isolation of high-quality plasmid DNA from Lactococcus and Lactobacillus spp. Appl Environ Microbiol 59:2730–2733PubMedPubMedCentralGoogle Scholar
  17. Seddik HA, Bendali F, Gancel F, Fliss I, Spano G, Drider D (2017) Lactobacillus plantarum and its probiotic and food potentialities. Probiotics Antimicrob Proteins 9:111–122CrossRefGoogle Scholar
  18. Serrano LM, Molenaar D, Wels M, Teusink B, Bron PA, Vos WMD, Smid EJ (2007) Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1. Microb Cell Fact 6:29CrossRefGoogle Scholar
  19. Siezen RJ, van Hylckama Vlieg JE (2011) Genomic diversity and versatility of Lactobacillus plantarum, a natural metabolic engineer. Microb Cell Fact 10(Suppl 1):S3CrossRefGoogle Scholar
  20. Siezen RJ, Tzeneva VA, Castioni A, Wels M, Phan HT, Rademaker JL, Starrenburg MJ, Kleerebezem M, Molenaar D, van Hylckama Vlieg JE (2010) Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches. Environ Microbiol 12:758–773CrossRefGoogle Scholar
  21. Siezen RJ, Francke C, Renckens B, Boekhorst J, Wels M, Kleerebezem M, van Hijum SA (2012) Complete resequencing and reannotation of the Lactobacillus plantarum WCFS1 genome. J Bacteriol 194:195–196CrossRefGoogle Scholar
  22. Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN, Rao BS, Smirnov S, Sverdlov AV, Vasudevan S, Wolf YI, Yin JJ, Natale DA (2003) The COG database: an updated version includes eukaryotes. BMC Bioinf 4:41CrossRefGoogle Scholar
  23. Touati D (2000) Iron and oxidative stress in bacteria. Arch Biochem Biophys 373:1–6CrossRefGoogle Scholar
  24. Wang Y, Chen C, Ai L, Zhou F, Zhou Z, Wang L, Zhang H, Chen W, Guo B (2011) Complete genome sequence of the probiotic Lactobacillus plantarum ST-III. J Bacteriol 193:313–314CrossRefGoogle Scholar
  25. Zhang YH, Xu D, Zhao XH, Song Y, Liu YL, Li HN (2016) Biodegradation of two organophosphorus pesticides in whole corn silage as affected by the cultured Lactobacillus plantarum. 3 Biotech 6:73CrossRefGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.Key Laboratory of Functional DairyCo-constructed by Ministry of Education and Beijing MunicipalityBeijingChina

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