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3 Biotech

, 9:130 | Cite as

Functional characterization of the rice root Germin-like protein gene-1 (OsRGLP1) promoter in Nicotiana tabacum

  • Muhammad IlyasEmail author
  • Wasim Akhtar
  • Shazia Rehman
  • Syed Muhammad Saqlan Naqvi
  • Tariq Mahmood
Original Article
  • 89 Downloads

Abstract

Germin (GER) and germin-like protein (GLP) genes play a very important role against various stresses. Promoter analysis provide significant insight into gene’s function and regulation. Presently, upstream region (1228 bp) of the OsRGLP1 gene was functionally characterized via heterologous expression. It was fused with the Glucuronidase (GUS) reporter gene and the expression cassette was used to transform Nicotiana tabacum using Agrobacterium-mediated transformation. Transgenic plants were examined via quantitative real-time PCR (qPCR) to analyze its role in wounding, salinity, drought, abscisic acid (ABA) and circadian rhythm. OsRGLP1 was highly induced by ABA and drought by showing 28- and 25-fold changes in GUS mRNA level respectively as compared to wounding (fourfold change) and salinity (threefold change). However, no activity was observed in circadian rhythm. Histochemically, strong GUS activity was observed in leaf veins, midrib, epidermal hair, stomata guard cells, stem cortex, root hairs, xylem and phloem and at cellular level in cell wall, cytoplasm and its periphery. OsRGLP1 promoter can be used to develop agronomically important transgenic plants in future food program.

Keywords

Rice germin-like proteins OsRGLP1 Germin Germin-like protein Promoter GUS Stress 

Notes

Acknowledgements

We are thankful to The Higher Education Commission (HEC) of Pakistan for providing financial assistance. Grant no.: 17-5-(2BM1-351)HEC/Sch-ind/2012.

Author contributions

MI and TM conducted experiments while SR, WA and SMSN helped in manuscript writing.

Compliance with ethical standards

Conflict of interest

The authors declared that they have no conflict of interest in the publication.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Muhammad Ilyas
    • 1
    Email author
  • Wasim Akhtar
    • 4
  • Shazia Rehman
    • 5
  • Syed Muhammad Saqlan Naqvi
    • 3
  • Tariq Mahmood
    • 2
  1. 1.Department of BotanyUniversity of SwabiAnbar SwabiPakistan
  2. 2.Department of Botany, Faculty of Biological sciencesQuaid-i-Azam UniversityIslamabadPakistan
  3. 3.Bacha Khan UniversityCharsaddaPakistan
  4. 4.Department of BotanyMohi-Ud-Din Islamic UniversityAzad Jammu and KashmirPakistan
  5. 5.Department of BotanyUniversity of Gujrat sub campus RawalpindiRawalpindiPakistan

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