BaCf3: highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens
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In the present study, we characterized bacteriocin BaCf3, isolated and purified from Bacillus amyloliquefaciens BTSS3, and demonstrated its inhibitory potential on growth and biofilm formation of certain food spoilage bacteria and pathogens. Purification was by gel filtration chromatography and its molecular weight was 3028.422 Da after MALDI-TOF MS. The bacteriocin was highly thermostable withstanding even autoclaving conditions and pH tolerant (2.0–13.0). The bacteriocin was sensitive to oxidizing agent (DMSO) and reducing agent (DTT). The de novo sequence of the bacteriocin BaCf3 was identified and was found to be novel. The sequence analysis shows the presence of a disulphide linkage between C6 and C13. The microtitre plate assay proved that BaCf3 could reduce up to 80% biofilm produced by strong biofilm producers from food samples. In addition, BaCf3 did not show cytotoxicity on 3-TL3 cell line.
KeywordsBacteriocin Bacillus MALDI-TOF Biofilm Cytotoxicity
The authors acknowledge project grants from Centre for Marine Living Resources & Ecology-Ministry of Earth Sciences, Government of India (MOES/10-MLR/2/2007 and MOES/10-MLR-TD/03/2013) given to Dr. Sarita G Bhat, Dept. of Biotechnology, Cochin University of Science and Technology, Kochi, India. The authors also acknowledge Dr. Laxmi. M for providing the biofilm producers.
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest.
- Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH, Boyd MR (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res 48:589–601Google Scholar
- Goh HF, Philip K (2015) Purification and characterization of bacteriocin produced by Weissella confusa A3 of dairy origin. PLoS One 10:0140434Google Scholar
- Laxmi M, Sarita GB (2014) Diversity characterization of biofilm forming microorganisms in food sampled from local markets in Kochi, Kerala, India. Int J Recent Sci Res 5:1070–1075Google Scholar
- Laxmi M, Kurian NK, Smitha S, Bhat SG (2016) Melanin and bacteriocin from marine bacteria inhibit biofilms of foodborne pathogens. Ind J Biotechnol 15:392–399Google Scholar
- Vincent SS, John SB (2009) Buffers: principles and practice. In: Richard RB, Murray PD (eds) Methods in enzymology. Elsevier Science & Technology Books, Massachusetts, pp 43–56Google Scholar
- Wilson K, Walker J (eds) (2000) Principles and techniques of practical biochemistry. Cambridge University Press, CambridgeGoogle Scholar
- World Health Organization (2015) WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015. World Health Organization No. 9789241565165Google Scholar