3 Biotech

, 9:136 | Cite as

BaCf3: highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens

  • E. S. Bindiya
  • K. J. Tina
  • Raghul Subin Sasidharan
  • Sarita G. BhatEmail author
Short Reports


In the present study, we characterized bacteriocin BaCf3, isolated and purified from Bacillus amyloliquefaciens BTSS3, and demonstrated its inhibitory potential on growth and biofilm formation of certain food spoilage bacteria and pathogens. Purification was by gel filtration chromatography and its molecular weight was 3028.422 Da after MALDI-TOF MS. The bacteriocin was highly thermostable withstanding even autoclaving conditions and pH tolerant (2.0–13.0). The bacteriocin was sensitive to oxidizing agent (DMSO) and reducing agent (DTT). The de novo sequence of the bacteriocin BaCf3 was identified and was found to be novel. The sequence analysis shows the presence of a disulphide linkage between C6 and C13. The microtitre plate assay proved that BaCf3 could reduce up to 80% biofilm produced by strong biofilm producers from food samples. In addition, BaCf3 did not show cytotoxicity on 3-TL3 cell line.


Bacteriocin Bacillus MALDI-TOF Biofilm Cytotoxicity 



The authors acknowledge project grants from Centre for Marine Living Resources & Ecology-Ministry of Earth Sciences, Government of India (MOES/10-MLR/2/2007 and MOES/10-MLR-TD/03/2013) given to Dr. Sarita G Bhat, Dept. of Biotechnology, Cochin University of Science and Technology, Kochi, India. The authors also acknowledge Dr. Laxmi. M for providing the biofilm producers.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest.

Supplementary material

13205_2019_1639_MOESM1_ESM.docx (134 kb)
Supplementary material 1 (DOCX 134 KB)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • E. S. Bindiya
    • 1
  • K. J. Tina
    • 1
  • Raghul Subin Sasidharan
    • 1
  • Sarita G. Bhat
    • 1
    Email author
  1. 1.Department of BiotechnologyCochin University of Science and TechnologyCochinIndia

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