An optimized method to obtain high-quality RNA from cassava storage root
Extracting RNA with high quality and integrity is crucial for molecular biology studies in eukaryotes. However, RNA isolation from cassava storage root raises a great concern because it contains large amounts of polysaccharides and polyphenol compounds. In the current study, four RNA extraction methods were evaluated for extracting RNA from cassava storage root. We found that the modified TM method (MTM) is timesaving and low-cost extraction method with high quality and quantities of RNA. The effectiveness of the improved method was assessed for qPCR analysis of four selected genes from total RNA of storage root. The improved protocol generated 4.18–5.94 µg RNA/g fresh weight. An A260/280 ratios of RNA samples are ranged from 2.14 to 2.17. The RIN values are ranged from 7.2 to 8.0. Importantly, isolated total RNA by MTM was successfully used for library construction and transcriptome sequencing. Therefore, we provide an efficient and low-cost method, MTM, for extracting high quality and quantities of RNA from cassava storage root.
KeywordsRNA isolation Cassava storage root High polysaccharides
This work was supported by grants from the National Key R&D Program of China (2018YFD1000500), National Natural Science Foundation of China (31670250), the startup funding of Hainan University (KYQD1562) to ZW, and project of cultivating talents for outstanding undergraduate students in crop science of Hainan University.
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
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