3 Biotech

, 9:60 | Cite as

Genetic diversity and structure of Dendrocalamus hamiltonii natural metapopulation: a commercially important bamboo species of northeast Himalayas

  • Rajendra K. MeenaEmail author
  • Maneesh S. Bhandhari
  • Santan Barhwal
  • Harish S. Ginwal
Original Article


Dendrocalamus hamiltonii is a commercially important bamboo species of India, experiencing population depletion due to heavy extraction from natural forests. Nuclear simple sequence repeats (nSSRs) were used to study the genetic diversity and population genetic structure of 19 natural stands of D. hamiltonii distributed across the northeast Himalayas. A total of 68 nSSR primer pairs of D. latiflorus and Bambusa arundinacea have been tested in D. hamiltonii for their transferability, out of which 17 primers showing positive and polymorphic amplification were used for genotyping. A total of 130 alleles were generated in 535 individuals of all the populations using selected primer pairs. The marker analysis indicated that D. hamiltonii populations have maintained a low level of genetic diversity (h = 0.175, I = 0.291) in northeastern region of India. Despite a large proportion of the genetic variation (83.47%) confined within the populations, a moderate level of genetic differentiation (FST = 0.165) was observed among the populations. The clustering pattern obtained in UPGMA and STRUCTURE analysis revealed that most of the populations were clustered in accordance with their geographical distribution. Two populations (DH03 and DH13) exhibiting significant genetic admixture were identified and recommended for in situ conservation. In addition, six highly diverse populations were also suggested for conservation in different geographical area under study. The study has revealed useful nSSR markers for D. hamiltonii, which were lacking earlier and the information generated herein is of paramount importance in devising programs for species conservation and genetic improvement.


Bamboo Dendrocalamus hamiltonii Genetic diversity Genetic differentiation Genetic structure nSSR 



The authors are thankful to Science Engineering and Research Board (SERB), New Delhi, India, for providing financial support. The field and laboratory facilities provided by Directors (RFRI, Jorhat and FRI, Dehradun) for execution of work is also duly acknowledged. We are thankful to the forest departments of Mizoram, Assam, Nagaland, Meghalaya and Arunachal Pradesh for granting necessary permissions and providing support during sample collection.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary material

13205_2019_1591_MOESM1_ESM.pdf (335 kb)
Supplementary material 1 (PDF 335 KB)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Division of Genetics and Tree ImprovementForest Research InstituteDehradunIndia

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