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3 Biotech

, 9:56 | Cite as

Enzymatic hydrolysis of tropical weed xylans using xylanase from Aureobasidium melanogenum PBUAP46 for xylooligosaccharide production

  • Tanutcha Patipong
  • Pongtharin LotrakulEmail author
  • Panuwat Padungros
  • Hunsa Punnapayak
  • Wichanee Bankeeree
  • Sehanat PrasongsukEmail author
Original Article
  • 20 Downloads

Abstract

The maximum yield of xylanase from Aureobasidium melanogenum PBUAP46 was 5.19 ± 0.08 U ml−1 when cultured in a production medium containing 3.89% (w/v) rice straw and 0.75% (w/v) NaNO3 as carbon and nitrogen sources, respectively, for 72 h. This enzyme catalyzed well and was relatively stable at pH 7.0 and room temperature (28 ± 2 °C). The produced xylanase was used to hydrolyze xylans from four tropical weeds, whereupon it was found that the highest amounts of reducing sugars in the xylan hydrolysates of cogon grass (Imperata cylindrical), Napier grass (Pennisetum purpureum), and vetiver grass (Vetiveria zizanioides) were at 20.44 ± 0.84, 17.50 ± 0.29, and 19.44 ± 0.40 mg 100 mg xylan−1, respectively, but it was not detectable in water hyacinth (Eichhornia crassipes) hydrolysate. The highest combined amount of xylobiose and xylotriose was obtained from vetiver grass; thus, it was selected for further optimization. After optimization, xylanase digestion of vetiver grass xylan at 27.94 U g xylan−1 for 92 h 19 min gave the highest amount of reducing sugars (23.65 ± 1.34 mg 100 mg xylan−1), which were principally xylobiose and xylotriose. The enriched XOs exhibited a prebiotic property, significantly stimulating the growth of Lactobacillus brevis and L. casei by a factor of up to 3.5- and 6.5-fold, respectively, compared to glucose.

Keywords

Tropical weed Xylan Xylanase Xylooligosaccharide Prebiotic 

Notes

Funding

This study was financially supported by the Development and Promotion of Science and Technology talents project (DPST), and the Asia Research Center, Chulalongkorn University.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

13205_2019_1586_MOESM1_ESM.docx (110 kb)
Supplementary material 1 (DOCX 109 KB)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Plant Biomass Utilization Research Unit, Department of Botany, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  2. 2.Program in Biotechnology, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  3. 3.Department of Chemistry, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  4. 4.Department of Biology, Faculty of Science and TechnologyUniversitas AirlanggaSurabayaIndonesia

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