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3 Biotech

, 9:67 | Cite as

Low-cost shoot multiplication and improved growth in different cultivars of Canna indica

  • Dharmendra Kumar Purshottam
  • Ramesh Kumar Srivastava
  • Pratibha MisraEmail author
Original Article
  • 18 Downloads

Abstract

Canna (Canna indica L.) is an ornamental landscape plant used specially for the garden borders and beds. It grows in tropical and subtropical countries including India. Canna is a less explored crop, mainly because it is a slow growing monocot with extremely hard seed coat and difficult to establish in vitro, as bacterial contamination is carried through the soil-grown rhizome. Many cultivars (ca. 150) of canna are being maintained in the garden germplasm of National Botanical Research Institute. To obtain 100% in vitro seed germination, chipping off of seeds with a sterilized nail clipper and soaking for 24–48 h or until radical emergence was a prerequisite. To obtain a foolproof tissue culture protocol of canna, in the present study, shoot multiplication was obtained through rhizome axillary buds. Among semisolid, liquid submerged and liquid media with glass beads, the highest multiplication of shoots (10) was obtained in liquid media with glass beads in ‘Canna Flaccida’ cv. within 6 weeks of culture incubation. During a comparative analysis of shoot regeneration among ten most attractive selected cultivars of canna, two did not respond, whereas a significant difference was obtained among eight cultivars. The regenerated shoots were rooted, acclimatized, and transferred to the pots, where they grew normally.

Keywords

Cultivars (cvs.) Glass beads Rhizome axillary buds In vitro-seed germination Shoot regeneration Canna indica L. 

Notes

Acknowledgements

The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow, for the facilities provided. This study was funded by Council of Scientific and Industrial Research-NBRI, through the Project no. OLP-0088.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest with the authors.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Plant BiotechnologyCSIR-National Botanical Research Institute (NBRI)LucknowIndia
  2. 2.CSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndia

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