3 Biotech

, 9:53 | Cite as

Phyto anti-biofilm elicitors as potential inhibitors of Helicobacter pylori

  • Ashwini Prasad
  • Aishwarya Tripurasundari Devi
  • M. N. Nagendra Prasad
  • Farhan Zameer
  • Govindaraju Shruthi
  • Chandan ShivamalluEmail author
Original Article


Helicobacter pylori (H. pylori) infection is a global public health concern. Due to its high adaptability in various adverse environments (temperature, pH, adhesion, phenotypic forms), targeting the bacterium is quite challenging. Moreover, due to its high persistence, decreased patience compliance and emerging antibiotic resistance, researchers have been forced to search for novel candidates with lesser or no side effects. Hence, in the current study, phytobioactives have been screened for its anti-biofilm attributes against H.pylori. Gastric biopsy samples have been screened using confirmatory techniques (microbiological, biochemical and molecular) for their virulent and non-virulent biomarkers. Physico-nutritive parameters were standardized. H. pylori biofilms were assessed using microtitre plate assay. Biofilms’ biomass and exopolysaccharide have been evaluated using crystal violet and ruthenium red staining, respectively. Anti-biofilm screening was performed using potent aqueous phytochemicals namely Acorus calamus, Colocasia esculenta and Vitex trifolia. The results indicated the confluent growth of the H. pylori biofilms confirmed through genotyping and grew best at 37 °C for 72 h at a pH of 7.5 on polystyrene plates. Further, among the phytochemicals tested, Acorus calamus exhibited the highest H. pylori anti-biofilm activity via a dose-dependent pattern. The overall observations of the study will pave way for newer approaches to understand and combat bacterial pathogenesis and will contribute towards better health and hygiene.


Helicobacter pylori Sessile bacteria Biofilm biomass Virulent cascade Microtiter plate biofilm assay 


Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest.

Supplementary material

13205_2019_1582_MOESM1_ESM.docx (64 kb)
Supplementary material 1 (DOCX 64 KB)


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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  • Ashwini Prasad
    • 1
  • Aishwarya Tripurasundari Devi
    • 2
  • M. N. Nagendra Prasad
    • 2
  • Farhan Zameer
    • 3
  • Govindaraju Shruthi
    • 1
  • Chandan Shivamallu
    • 1
    Email author
  1. 1.Department of Water and Health Faculty of Life SciencesJSS Academy of Higher Education & ResearchMysoreIndia
  2. 2.Department of BiotechnologySri Jayachamarajendra College of Engineering, JSS Science and Technology UniversityMysoreIndia
  3. 3.Department of Biochemistry, School of Basic and Applied SciencesDayananda Sagar UniversityBangaloreIndia

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