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Diesel degrading bacterial endophytes with plant growth promoting potential isolated from a petroleum storage facility

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Abstract

Thirteen (13) endophytic bacterial strains were isolated from Echinochloa crus-galli (Cockspur grass) and Cynodon dactylon (Bermuda grass) growing in an oil-contaminated site at a petroleum storage and transportation facility. Of the 13 strains assessed for their potential to degrade monoaromatic compounds (phenol, toluene, and xylene) and diesel and for their plant growth promoting (PGP) ability (phosphate solubilization, siderophores and 1-aminocyclopropane-1-carboxylate (ACC) deaminase production), isolate J10 (identified as Pseudomonas sp. by 16S rRNA gene sequencing) was found to the best diesel biodegrader with the best PGP traits. The Monod model used for Pseudomonas sp. J10 growth kinetics on diesel fuel as the sole carbon source showed that the maximum specific bacterial growth rate was 0.0644 h− 1 and the half velocity constant (Ks) was estimated as 4570 mg L− 1. The overall growth yield coefficient and apparent growth yield were determined to be 0.271 g h− 1 and 0.127 g cells/g substrate, respectively. Pseudomonas sp. J10 removed 69% diesel in four days as determined by gas chromatographic (GC) analysis. These findings could assist in developing an endophyte assisted efficient diesel biodegradation system using Pseudomonas sp. J10 isolated from Echinochloa crus-galli.

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Acknowledgements

The authors would like to acknowledge the Higher Education Commission of Pakistan for financing this research through International Research Support Initiative Program. We thank Dr. Sergio Capareda and Dr. Amado Maglinao Jr. for Gas Chromatographic analysis.

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Authors and Affiliations

  1. Institute of Environmental Sciences and Engineering, School of Civil and Environmental Engineering, National University of Sciences and Technology, Sector H-12, Islamabad, 44000, Pakistan

    Aneela Iqbal & Muhammad Arshad

  2. Biological and Agricultural Engineering, Texas A&M University, College Station, TX, 77843, USA

    Aneela Iqbal & Raghupathy Karthikeyan

  3. Soil and Crop Sciences, Texas A&M University, College Station, TX, 77843, USA

    Aneela Iqbal, Terry J. Gentry & Arthur Paul Schwab

  4. Department of Environmental Science, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Jamshaid Rashid

  5. Bio-resource Conservation Institute (BCI), National Culture Collection of Pakistan (NCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Iftikhar Ahmed

Authors
  1. Aneela Iqbal
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  2. Muhammad Arshad
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  3. Raghupathy Karthikeyan
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  4. Terry J. Gentry
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  5. Jamshaid Rashid
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  6. Iftikhar Ahmed
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  7. Arthur Paul Schwab
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Contributions

AI: performed lab and field work, analyzed the data and wrote the manuscript. MA: supervised, advised lab work and data analyses. RK: advised biodegradation experiments, GC analysis and data analyses. TJG: advised lab work, experimental design and data analysis, JR: helped in GC and kinetic analysis, IA: helped in phylogenetic analysis of the strain, APS: supervised field work and data analyses.

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Correspondence to Muhammad Arshad.

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Iqbal, A., Arshad, M., Karthikeyan, R. et al. Diesel degrading bacterial endophytes with plant growth promoting potential isolated from a petroleum storage facility. 3 Biotech 9, 35 (2019). https://doi.org/10.1007/s13205-018-1561-z

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