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3 Biotech

, 9:35 | Cite as

Diesel degrading bacterial endophytes with plant growth promoting potential isolated from a petroleum storage facility

  • Aneela Iqbal
  • Muhammad ArshadEmail author
  • Raghupathy Karthikeyan
  • Terry J. Gentry
  • Jamshaid Rashid
  • Iftikhar Ahmed
  • Arthur Paul Schwab
Original Article

Abstract

Thirteen (13) endophytic bacterial strains were isolated from Echinochloa crus-galli (Cockspur grass) and Cynodon dactylon (Bermuda grass) growing in an oil-contaminated site at a petroleum storage and transportation facility. Of the 13 strains assessed for their potential to degrade monoaromatic compounds (phenol, toluene, and xylene) and diesel and for their plant growth promoting (PGP) ability (phosphate solubilization, siderophores and 1-aminocyclopropane-1-carboxylate (ACC) deaminase production), isolate J10 (identified as Pseudomonas sp. by 16S rRNA gene sequencing) was found to the best diesel biodegrader with the best PGP traits. The Monod model used for Pseudomonas sp. J10 growth kinetics on diesel fuel as the sole carbon source showed that the maximum specific bacterial growth rate was 0.0644 h− 1 and the half velocity constant (Ks) was estimated as 4570 mg L− 1. The overall growth yield coefficient and apparent growth yield were determined to be 0.271 g h− 1 and 0.127 g cells/g substrate, respectively. Pseudomonas sp. J10 removed 69% diesel in four days as determined by gas chromatographic (GC) analysis. These findings could assist in developing an endophyte assisted efficient diesel biodegradation system using Pseudomonas sp. J10 isolated from Echinochloa crus-galli.

Keywords

Endophytic bacteria Diesel Phenol Toluene Xylene Phytoremediation 

Notes

Acknowledgements

The authors would like to acknowledge the Higher Education Commission of Pakistan for financing this research through International Research Support Initiative Program. We thank Dr. Sergio Capareda and Dr. Amado Maglinao Jr. for Gas Chromatographic analysis.

Author contributions

AI: performed lab and field work, analyzed the data and wrote the manuscript. MA: supervised, advised lab work and data analyses. RK: advised biodegradation experiments, GC analysis and data analyses. TJG: advised lab work, experimental design and data analysis, JR: helped in GC and kinetic analysis, IA: helped in phylogenetic analysis of the strain, APS: supervised field work and data analyses.

Compliance with ethical standards

Conflict of interest

Authors declare that there are no competing financial as well as individual interests.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Institute of Environmental Sciences and Engineering, School of Civil and Environmental EngineeringNational University of Sciences and TechnologyIslamabadPakistan
  2. 2.Biological and Agricultural EngineeringTexas A&M UniversityCollege StationUSA
  3. 3.Soil and Crop SciencesTexas A&M UniversityCollege StationUSA
  4. 4.Department of Environmental Science, Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabadPakistan
  5. 5.Bio-resource Conservation Institute (BCI)National Culture Collection of Pakistan (NCCP), National Agricultural Research Centre (NARC)IslamabadPakistan

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