Preparation and characterization of sericin powder extracted with deep sea water
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In this study, we developed an effective technology for the extraction of sericin from silk cocoons by deep sea water (DSW). We focused on extraction of sericin in the absence of chemical additives to obtain a safe, effective, inexpensive sericin powder. Sericin was extracted using a simple high-temperature process involving heating, condensation with Molus alba, filtering with cotton cloth, cold storage, and lyophilization. The results showed that the yield of sericin (26%) extracted by DSW was approximately 2% higher than that obtained using a chemical buffer (0.2 M Na2CO3, 24%). The marine mineral sericin M. alba (MSM) showed a size distribution of 15–250 kDa, with major peaks at 75–250 kDa with a galactose chain. Additionally, this MSM product had high antioxidant, whitening, and antibiosis effects and could be safely stored for a long time. Thus, our findings supported the use of a DSW extraction method, which was ecofriendly and yielded a proteinous, biodegradable biopolymer, for preparation of sericin.
KeywordsSilk Sericin Ecofriendly extraction Biomaterial Antibiosis
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA, 117063-3) and the Technological Innovation R&D Program of Small and Medium Business Administration (C0453809).
Compliance with ethical standards
Conflict of interest
There are no financial/commercial or any other conflicts of interest among authors.
- Abhilasha R, Lalit J (2015) The biopolymer sericin: extraction and applications. J Text Sci Eng 5(1):188–193Google Scholar
- Bianchi AS, Colonna GM (1992) Developments in the degumming of silk. Melliand Textilberichte 1:68–75Google Scholar
- Gulrajani ML, Sethi B, Kapur V (1992) Application of chrome mordant dyes on silk. Colourage 39(10):38–42Google Scholar
- Gulrajani ML, Gupta SV, Gupta A (1996) Degumming of silk with different protease enzymes. Indian J Fibre Text Res 24(4):270–275Google Scholar
- Mashhiro S, Hideyuk Y, Norihisa K (2000) Consumption of silk protein, sericin elevates intestinal absorption of zinc, iron, magnesium and calcium in rats. Nutr Res 20:1505–1511Google Scholar
- Rangam R, Wang L, Kanwa JR, Wang X (2010) Molecular weight and secondary structure change in eri silk during alkali degumming and powdering. Appl Polym 119:1339–1347Google Scholar
- Rangi A, Jajpura L (2015) The biopolymer sericin: extraction and applications. J Text Sci Eng 5:1–5Google Scholar
- Samihah ZMH, Majid FAA, Jaafar AB, Mahdzir A, Musa MN (2016) Potential health benefits of deep sea water. Evid Based Complement Altern Med 26:18Google Scholar
- Sarovar S, Sudatis B, Meesilpa P, Grady BP, Magaraphan R (2003) The use of sericin as an antioxidant and antimicrobial for polluted air treatment. Rev Adv Mater Sci 5:193–198Google Scholar