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3 Biotech

, 9:30 | Cite as

Preparation and characterization of sericin powder extracted with deep sea water

  • Sun Mee HongEmail author
  • Sung Chang Choi
  • Hyun Mee Park
  • Young Sik Seok
Original Article
  • 47 Downloads

Abstract

In this study, we developed an effective technology for the extraction of sericin from silk cocoons by deep sea water (DSW). We focused on extraction of sericin in the absence of chemical additives to obtain a safe, effective, inexpensive sericin powder. Sericin was extracted using a simple high-temperature process involving heating, condensation with Molus alba, filtering with cotton cloth, cold storage, and lyophilization. The results showed that the yield of sericin (26%) extracted by DSW was approximately 2% higher than that obtained using a chemical buffer (0.2 M Na2CO3, 24%). The marine mineral sericin M. alba (MSM) showed a size distribution of 15–250 kDa, with major peaks at 75–250 kDa with a galactose chain. Additionally, this MSM product had high antioxidant, whitening, and antibiosis effects and could be safely stored for a long time. Thus, our findings supported the use of a DSW extraction method, which was ecofriendly and yielded a proteinous, biodegradable biopolymer, for preparation of sericin.

Keywords

Silk Sericin Ecofriendly extraction Biomaterial Antibiosis 

Notes

Acknowledgements

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA, 117063-3) and the Technological Innovation R&D Program of Small and Medium Business Administration (C0453809).

Compliance with ethical standards

Conflict of interest

There are no financial/commercial or any other conflicts of interest among authors.

Supplementary material

13205_2018_1558_MOESM1_ESM.pdf (77 kb)
Suppl. Fig 1. Procedures for the preparation of MSM (marine mineral sericin) from M. alba extracted by DSW (PDF 77 KB)
13205_2018_1558_MOESM2_ESM.pdf (143 kb)
Suppl. Fig. 2. Antimicrobial effects of sericin extracts. A, Effects on Bacillus sp. and Staphylococcus sp. B, Effects on skin- or scalp-related microorganisms (Propionibacterium acnes and Malassezia furfur). The red circle and arrows indicate antimicrobial activity. Each experiment was performed in triplicate (PDF 142 KB)
13205_2018_1558_MOESM3_ESM.pdf (59 kb)
Suppl. Fig. 3. Stability of microbes in sericin powder during storage at 4°C for 6 months. DW, distilled water used as a solvent for dissolving the powder; CS, sericin extracted by sodium carbonate buffer (Na2CO3); MS, marine mineral sericin extracted by DSW; MSM, marine mineral sericin M. alba extracted by DSW and then condensed with M. alba (PDF 59 KB)

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Technology DevelopmentGIMBUljinRepublic of Korea
  2. 2.Sericulture and Entomology Experiment StationHongcheonRepublic of Korea

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