Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography
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In this study, Gelidium elegans is investigated for ethanol production. A combination of factors including different temperatures, acid concentration and incubation time was evaluated to determine the suitable saccharification conditions. The combination of 2.5% (w/v) H2SO4 at 120 °C for 40 min was selected for hydrolysis of the seaweed biomass, followed by purification, and fermentation to yield ethanol. The galactose and glucose were dominant reducing sugars in the G. elegans hydrolysate and under optimum condition of dilute acid hydrolysis, 39.42% of reducing sugars was produced and fermentation resulted in ethanol concentration of 13.27 ± 0.47 g/L. A modified method was evaluated for sample preparation for gas chromatography (GC) analysis of the ethanol content. A solvent mixture of acetonitrile and iso-butanol precipitated dissolved organic residues and reduced water content in GC samples at least by 90%. Results showed that this method could be successfully used for bioethanol production from seaweed.
KeywordsG. elegans Seaweed Sample preparation Gas chromatography Bioethanol
National institute of technology evaluation
Yeast potato dextrose
Flame ionisation detector
Authors acknowledge University of Malaya for providing University of Malaya Research Grant (Project no: PV026-2012), GC002B-15SBS and HICoE Grant IOES-2014F. We are grateful to the Algae Research Laboratory, University of Malaya members, specifically my good friends Dr. Bahram Barati, Dr. Vejeysri Vello and Mr. Hamed Nassrolahi who assisted us in this research work.
Compliance with ethical standards
Conflict of interest
Authors declare no conflict of interest.
- Canfield DV, Smith MD, Adams HJ, Houston ER (1998) Selection of an Internal Standard for postmortem ethanol analysis. Civil Aeromedical Institute, Federal Aviation Administration, OklahamaGoogle Scholar
- Kuhn ER (2002) Water injections in GC how wet can you get? LCGC Asia Pac 5(3):30–32Google Scholar
- Taherzadeh MJ, Karimi K (2007) Acid-based hydrolysis processes for ethanol from lignocellulosic materials: a review. BioResources 2(3):472–499Google Scholar
- Timson DJ (2007) Galactose metabolism in Saccharomyces cerevisiae. Dyn Biochem Process Biotechnol Mol Biol 1(1):63–73Google Scholar