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3 Biotech

, 9:15 | Cite as

Stochasticity in transcriptional expression of a negative regulator of Arabidopsis ABA network

  • Necla PehlivanEmail author
Original Article
  • 26 Downloads

Abstract

Stably heritable spatiotemporal co/over-expression of distinct transcriptional regulators across generations is a desired target as they signal traffic in the cell. Here, the stability and expression pattern of AtHB7 (Arabidopsis homeodomain-leucine zipper class I) cDNA was characterized in 220 random population of transformed tomato clones where no AtHB7 orthologous has been identified in to date. Integration of p35S::AtHB7 casette was tested by the amplification of the stretches (700/425 bp) in the target by NPT II/AtHB7 oligos. Transcriptional expression pattern for the amplicons of the specific transcripts in the leaf tissues of transformants were determined by qRT-PCR. Transgene copy number was negatively correlated with transgene expression level, yet a majority of transformants (78%) carried single-copy of transgene. About 1:3 of the lines containing two-copy inserts showed less transcript expression. Heterologous CaMV 35S promoter drove AtHB7, illuminated no penalty on transgene expression levels, stability or plant phenotype under drought stress. Integration and expression analysis of transcription factors is of great significance for reliable prediction of gene dosing/functions in plant genomes so as to sustain breeding under abiotic stress to guarantee food security.

Keywords

HDZip AtHB7 Tomato 35S Trans-gene expression stability Drought 

Notes

Acknowledgements

I, the corresponding author express my sincere thanks to Dr. Rina Iannacone for her precious help in this work.

Compliance with ethical standards

Conflict of interest

No conflict of interest does exist between third parties.

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Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Biology DepartmentRecep Tayyip Erdogan UniversityRizeTurkey

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