An effective pest management approach in potato to combat insect pests and herbicide
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Insect pests and weeds incur significant yield losses to potato crop worldwide. The increasing crop losses provide impetus for the development of pest management strategy that is equally effective against insect pests and weeds. In the present study, a molecular approach was used to develop transgenic potato lines (cv. Marabel) effective against Colorado potato beetle (Leptinotarsa decemlineata Say), potato tuber moth (Phthorimaea operculella Z.) and Basta® application. Agrobacterium tumefaciens strain EHA105 harboring binary vector pTF101.1 containing cry1Ac gene under the control of 35S and AoPR1 promoters was used to infect leaf discs and internodal explants. Phosphinothrincin was used at optimal concentration (2 mg/l) for the screening of primary transformants. The standard molecular assays exhibited gene integration and expression in putative transgenics. Real-time data revealed up to ninefold high cry1Ac transcript levels, whereas cry protein amount was estimated to 0.4 ppm in primary transformants. The analysis of first tuber progeny showed proper integration cry1Ac and bar genes in subsequent progeny. The transgenic plants also showed tolerance to the application of Basta®. The efficacy of cry1Ac was evaluated by allowing larvae of Colorado potato beetle (CPB) and potato tuber moth (PTM) to feed on transgenic plants. Results revealed appreciable mortality levels of different larval instars of CPB (20–100%) and PTM (50–100%). Overall, our results exhibit the potential of these transgenic lines to be used in a potato breeding program with the purpose to control insect pests and weeds.
KeywordsPotato Insect pests Weeds Management Transgenics
Authors acknowledge Prof. Dr. Ayhan Gökçe, Mr. Muhammad Nadir Naqqash and Mr. Muhammad Saleem for allowing us to use facilities of entomology laboratory and helping us in establishing leaf bioassays. Potato cultivar Marabel was provided by Prof. Dr. Mehmet Emin Çalışkan to establish its shoot culture. Many Thanks to Dr. Ufuk Demirel who helped to interpret the results; critical read manuscript and gave valuable suggestions for its improvement. We also thank Prof. Dr. Sebahattin Özcan for providing us recombinant plasmids.
The data presented in the manuscript is MS thesis work of Mr. Abdul Naser Amiri who completed his studies under the supervision of Dr. Allah Bakhsh.
Compliance with ethical standards
Conflict of interest
Authors declare no conflict of interest.
- Ahmed HAA, Onarıcı S, Bakhsh A, Akdoğan G, Karakoç ÖC, Özcan SF, Aydın G, Aasim M, Ünlü L, Sancak C, Naimov S (2017) Targeted expression of insecticidal hybrid SN19 gene in potato leads to enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata Say) and tomato leafminer (Tuta absoluta Meyrick). Plant Biotechol Rep 11:315–329CrossRefGoogle Scholar
- Capinera JL (2001) Handbook of vegetable insects. Academic, New York, p 729Google Scholar
- Estrada MA, Zarka K, Cooper S, Coombs J, Douches DS, Grafius EJ (2007) Potato tuberworm (Lepidoptera: Gelichiidae) resistance in potato lines with the Bacillus thuringiensis cry1Ac gene and natural resistance. Hortic Sci 42:1306–1311Google Scholar
- Figueira E, Figueiredo L, MonteNeshich D (1994) Transformation of potato (Solanum tuberosum) cv. Mantiqueira using Agrobacterium tumefaciens and evaluation of herbicide resistance. Plant Cell Rep 13:666–670Google Scholar
- James C (2016) Global status of commercialized biotech/GM Crops. ISAAA Brief. ISAAAGoogle Scholar
- Lagnaoui A, Cañedo V, Douches DS (2001) Evaluation of Bt-cry1Ia1 (cryV) transgenic potatoes on two species of potato tuber moth, Phthorimaea operculella and Symmetrischema tangolias (Lepidoptera: Gelechiidae) in Peru. CIP Program Report 1999–2000. International Potato Center, Lima, pp 117–121Google Scholar
- Padegimas L, Shulga OA, Skryabin KG (2004) Herbicide phosphinothricin tolerance in transgenic plants Nicotiana tabacum and Solanum tuberosum. Mol Biol 28:437–443Google Scholar
- Pimentel D (2018) Pests and their control. In: Handbook of natural pesticides: methods. CRC Press, Boca Raton, pp 3–19Google Scholar
- Rao CK (2005) Transgenic Bt technology 3, expression of transgenes. http://www.monsanto.co.uk/news/ukshowlib.phtml?uid=9304. Accessed 25 Sept 2018
- Sharma SK (2013) Effect of cutworm population and shoot damage in potato on the tuber yield. Potato J 40:114–121Google Scholar
- Soto N, Enriquez GA, Ferreira A, Corrada M, Fuentes A, Tiel K, Pujol M (2007) Efficient transformation of potato stem segments from cv. Desiree using phosphinothricin as selection marker. Biotech Appl 24:139–144Google Scholar
- Tripathi B, Singh CM, Bhargava M (1989) Comparative efficacy of herbicides in potato under conditions of North-Western Himalayas. Pesticides 23:37–38Google Scholar
- Üremiş İ, Caliskan ME, Uludağ A, Caliskan S (2009) Weed management in early-season potato production in the Mediterranean conditions of Turkey. Bulg J Agric Sci 15:423–434Google Scholar
- Visser D (2005) Guide to potato pests and their natural enemies in South Africa. Arc-Roodeplaat Vegetable and Ornamental Plant Institute, Pretoria, p 105Google Scholar
- Yüceer ÜS, Kayım M (2012) Patates böceği (leptinotarsa decemlineata say.)’Ne dayanikli bitkiler elde etmek amaciyla patates (solanum tuberosum L.)’In genetik transformasyonu. Çukurova Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, pp 27–33Google Scholar
- Zimdahl RL (2007) Fundamental of weed science, 3rd ed. Academic Press, LondonGoogle Scholar