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3 Biotech

, 9:16 | Cite as

An effective pest management approach in potato to combat insect pests and herbicide

  • Abdul Naser Amiri
  • Allah BakhshEmail author
Original Article
  • 27 Downloads

Abstract

Insect pests and weeds incur significant yield losses to potato crop worldwide. The increasing crop losses provide impetus for the development of pest management strategy that is equally effective against insect pests and weeds. In the present study, a molecular approach was used to develop transgenic potato lines (cv. Marabel) effective against Colorado potato beetle (Leptinotarsa decemlineata Say), potato tuber moth (Phthorimaea operculella Z.) and Basta® application. Agrobacterium tumefaciens strain EHA105 harboring binary vector pTF101.1 containing cry1Ac gene under the control of 35S and AoPR1 promoters was used to infect leaf discs and internodal explants. Phosphinothrincin was used at optimal concentration (2 mg/l) for the screening of primary transformants. The standard molecular assays exhibited gene integration and expression in putative transgenics. Real-time data revealed up to ninefold high cry1Ac transcript levels, whereas cry protein amount was estimated to 0.4 ppm in primary transformants. The analysis of first tuber progeny showed proper integration cry1Ac and bar genes in subsequent progeny. The transgenic plants also showed tolerance to the application of Basta®. The efficacy of cry1Ac was evaluated by allowing larvae of Colorado potato beetle (CPB) and potato tuber moth (PTM) to feed on transgenic plants. Results revealed appreciable mortality levels of different larval instars of CPB (20–100%) and PTM (50–100%). Overall, our results exhibit the potential of these transgenic lines to be used in a potato breeding program with the purpose to control insect pests and weeds.

Keywords

Potato Insect pests Weeds Management Transgenics 

Notes

Acknowledgements

Authors acknowledge Prof. Dr. Ayhan Gökçe, Mr. Muhammad Nadir Naqqash and Mr. Muhammad Saleem for allowing us to use facilities of entomology laboratory and helping us in establishing leaf bioassays. Potato cultivar Marabel was provided by Prof. Dr. Mehmet Emin Çalışkan to establish its shoot culture. Many Thanks to Dr. Ufuk Demirel who helped to interpret the results; critical read manuscript and gave valuable suggestions for its improvement. We also thank Prof. Dr. Sebahattin Özcan for providing us recombinant plasmids.

Author contributions

The data presented in the manuscript is MS thesis work of Mr. Abdul Naser Amiri who completed his studies under the supervision of Dr. Allah Bakhsh.

Compliance with ethical standards

Conflict of interest

Authors declare no conflict of interest.

References

  1. Ahmed HAA, Onarıcı S, Bakhsh A, Akdoğan G, Karakoç ÖC, Özcan SF, Aydın G, Aasim M, Ünlü L, Sancak C, Naimov S (2017) Targeted expression of insecticidal hybrid SN19 gene in potato leads to enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata Say) and tomato leafminer (Tuta absoluta Meyrick). Plant Biotechol Rep 11:315–329CrossRefGoogle Scholar
  2. Alyokhin A, Mota-Sanchez D, Baker M, Snyder WE, Menasha S, Whalon M, Dively G, Moarsi WF (2015) The Red Queen in a potato field: integrated pest management versus chemical dependency in Colorado potato beetle control. Pest Manag Sci 71:343–356CrossRefGoogle Scholar
  3. Anayol E, Bakhsh A, Karakoç ÖC, Onarıcı S, Köm D, Aasim M, Özcan SF, Barpete S, Khabbazi SD, Önol B, Sancak C (2016) Towards better insect management strategy: restriction of insecticidal gene expression to biting sites in transgenic cotton. Plant Biotechnol Rep 10:83–94CrossRefGoogle Scholar
  4. Bakhsh A, Anayol E, Ozcan SF (2014) Comparison of transformation efficiency of five Agrobacterium tumefaciens strains in Nicotiana tabacum L.. Emir J Food Agric 26:259–264CrossRefGoogle Scholar
  5. Bakhsh A, Anayol E, Khabbazi SD, Karakoç ÖC, Sancak C, Özcan S (2016) Development of insect-resistant cotton lines with targeted expression of insecticidal gene. Arch Biol Sci 68:773–780CrossRefGoogle Scholar
  6. Bakhsh A, Dinc T, Hussain T, Demirel U, Aasim M, Çalışkan ME (2018) Development of transgenic tobacco lines with pyramided insect resistant genes. Turk J Biol 42:174–186CrossRefGoogle Scholar
  7. Beaujean A, Sangwan R, Lecardonnel A, Sangwan-Norreel B (1998) Agrobacterium-mediated transformation of three economically important potato cultivars using sliced internodal explants, an efficient protocol of transformation. J Exp Bot 49:1589–1595CrossRefGoogle Scholar
  8. Beddington J (2010) Food security: contributions from science to a new and greener revolution. Philos Trans R Soc Lond Biol Sci 365:61–71CrossRefGoogle Scholar
  9. Bravo A, Gill SS, Soberon M (2007) Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49:423–435CrossRefGoogle Scholar
  10. Capinera JL (2001) Handbook of vegetable insects. Academic, New York, p 729Google Scholar
  11. Christou P, Capell T, Kohli A, Gatehouse JA, Gatehouse AM (2006) Recent developments and future prospects in insect pest control in transgenic crops. Trends Plant Sci 11:302–308CrossRefGoogle Scholar
  12. Cooper SG, Douches DS, Grafius EJ (2004) Combining genetic engineering and traditional breeding to provide elevated resistance in potatoes to Colorado potato beetle. Entomol Exp Appl 112:37–46CrossRefGoogle Scholar
  13. Davidson MM, Takla MFG, Jacobs JME, Butler RC, Wratten SD, Conner AJ (2004) Transformation of potato (Solanum tuberosum) cultivars with a cry 1Ac9 gene confers resistance to potato tuber moth (Phthorimaea operculella). N Z J Crop Hortic Sci 32:39–50CrossRefGoogle Scholar
  14. Dhillon MK, Sharma HC (2009) Effects of Bacillus thuringiensis δ-endotoxins Cry1Ab and Cry1Ac on the coccinellid beetle, Cheilomenes sexmaculatus (Coleoptera, Coccinellidae) under direct and indirect exposure conditions. Biocontrol Sci Technol 19:407–420CrossRefGoogle Scholar
  15. Douglas CJ, Staneloni RJ, Rubin RA, Nester EW (1985) Identification and genetic analysis of an Agrobacterium tumefaciens chromosomal virulence region. J Bacteriol 161:850–860PubMedPubMedCentralGoogle Scholar
  16. Estrada MA, Zarka K, Cooper S, Coombs J, Douches DS, Grafius EJ (2007) Potato tuberworm (Lepidoptera: Gelichiidae) resistance in potato lines with the Bacillus thuringiensis cry1Ac gene and natural resistance. Hortic Sci 42:1306–1311Google Scholar
  17. Ferro DN, Lyon SM (1991) Colorado potato beetle (Coleoptera: Chrysomelidae) larval mortality: operative effects of Bacillus thuringiensis subsp. san diego. J Econ Entomol 84:806–809CrossRefGoogle Scholar
  18. Figueira E, Figueiredo L, MonteNeshich D (1994) Transformation of potato (Solanum tuberosum) cv. Mantiqueira using Agrobacterium tumefaciens and evaluation of herbicide resistance. Plant Cell Rep 13:666–670Google Scholar
  19. Gokce A, Isaacs R, Whalon ME (2012) Dose-response relationships for the antifeedant effects of Humulus lupulus extracts against larvae and adults of the Colorado potato beetle. Pest Manag Sci 68:476–481CrossRefGoogle Scholar
  20. Halterman D, Guenthner J, Collinge S, Butler N, Douches D (2015) Biotech potatoes in the 21st century: 20 years since the first biotech potato. Am J Potato 93:1–20CrossRefGoogle Scholar
  21. Hameed A, Tahir MN, Asad S, Bilal R, Van Eck J, Jander G, Mansoor S (2017) RNAi-mediated simultaneous resistance against three RNA viruses in potato. Mol Biotechnol 59:73–83CrossRefGoogle Scholar
  22. James C (2016) Global status of commercialized biotech/GM Crops. ISAAA Brief. ISAAAGoogle Scholar
  23. Kaplanoglu E, Chapman P, Scott IM, Donly C (2017) Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata. Sci Rep 7:1762CrossRefGoogle Scholar
  24. Kim KH, Kabir E, Jahan SA (2017) Exposure to pesticides and the associated human health effects. Sci Total Environ 575:525–535CrossRefGoogle Scholar
  25. Kos M, Van Loon JJ, Dicke M, Vet LE (2009) Transgenic plants as vital components of integrated pest management. Trends Biotechnol 27:621–627CrossRefGoogle Scholar
  26. Lagnaoui A, Cañedo V, Douches DS (2001) Evaluation of Bt-cry1Ia1 (cryV) transgenic potatoes on two species of potato tuber moth, Phthorimaea operculella and Symmetrischema tangolias (Lepidoptera: Gelechiidae) in Peru. CIP Program Report 1999–2000. International Potato Center, Lima, pp 117–121Google Scholar
  27. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real time quantitative PCR and the 2∆∆C(T) method. Methods 25:402–408CrossRefGoogle Scholar
  28. Mi X, Ji X, Yang J, Liang L, Si H, Wu J, Zhang N, Wang D (2015) Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle. Comptes Rendus Biol 338:443–450CrossRefGoogle Scholar
  29. Michaud D, Bernier-Vadnais N, Overney S, Yelle S (1995) Constitutive expression of digestive cysteine proteinase forms during development of the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Insect Bioch Mol Biol 25:1041–1048CrossRefGoogle Scholar
  30. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  31. Nain V, Jaiswal R, Dalal M, Ramesh B, Kumar PA (2005) Polymerase chain reaction analysis of transgenic plants contaminated by Agrobacterium. Plant Mol Biol Report 23:59–65CrossRefGoogle Scholar
  32. Nicot N, Hausman JF, Hoffman L, Evers D (2005) Housekeeping gene selection for real time PCR normalization in potato during biotic and abiotic stress. J Exp Bot 56:2907–2914CrossRefGoogle Scholar
  33. Padegimas L, Shulga OA, Skryabin KG (2004) Herbicide phosphinothricin tolerance in transgenic plants Nicotiana tabacum and Solanum tuberosum. Mol Biol 28:437–443Google Scholar
  34. Pimentel D (2018) Pests and their control. In: Handbook of natural pesticides: methods. CRC Press, Boca Raton, pp 3–19Google Scholar
  35. Rao CK (2005) Transgenic Bt technology 3, expression of transgenes. http://www.monsanto.co.uk/news/ukshowlib.phtml?uid=9304. Accessed 25 Sept 2018
  36. Rao AQ, Bakhsh A, Kiani S, Shahzad K, Shahid AA, Husnain T, Riazuddin S (2009) The myth of plant transformation. Biotechnol Adv 27:753–763CrossRefGoogle Scholar
  37. Rondon SI (2010) The potato tuberworm: a literature review of its biology, ecology, and control. Am J Potato Res 87:149–166CrossRefGoogle Scholar
  38. Selale H, Dağlı F, Mutlu N, Doğanlar S, Frary A (2017) Cry1Ac-mediated resistance to tomato leaf miner (Tuta absoluta) in tomato. Plant Cell Tissue Organ Cult 131:65–73CrossRefGoogle Scholar
  39. Sharma SK (2013) Effect of cutworm population and shoot damage in potato on the tuber yield. Potato J 40:114–121Google Scholar
  40. Soto N, Enriquez GA, Ferreira A, Corrada M, Fuentes A, Tiel K, Pujol M (2007) Efficient transformation of potato stem segments from cv. Desiree using phosphinothricin as selection marker. Biotech Appl 24:139–144Google Scholar
  41. Tripathi B, Singh CM, Bhargava M (1989) Comparative efficacy of herbicides in potato under conditions of North-Western Himalayas. Pesticides 23:37–38Google Scholar
  42. Üremiş İ, Caliskan ME, Uludağ A, Caliskan S (2009) Weed management in early-season potato production in the Mediterranean conditions of Turkey. Bulg J Agric Sci 15:423–434Google Scholar
  43. Veale MA, Slabbert MM, Van Emmenes L (2012) Agrobacterium-mediated transformation of potato cv. Mnandi for resistance to the potato tuber moth (Phthorimaea operculella). S Afr J Bot 80:7–74CrossRefGoogle Scholar
  44. Visser D (2005) Guide to potato pests and their natural enemies in South Africa. Arc-Roodeplaat Vegetable and Ornamental Plant Institute, Pretoria, p 105Google Scholar
  45. Westedt AL, Douches DS, Pett W, Grafius EJ (1998) Evaluation of natural and engineered resistance mechanisms in Solanum tuberosum L. for resistance to Phthorimaea operculella Zeller. J Econ Entomol 91:552–556CrossRefGoogle Scholar
  46. Wilson RC, Tegg SR (2012) In vitro cell selection techniques for enhancing disease resistance—case study: common scab resistance in Russet Burbank. In: He Z, Larkin R, Honeycutt W (eds) Sustainable potato production: global case studies. Springer, Heidelberg, pp 327–345CrossRefGoogle Scholar
  47. Yüceer ÜS, Kayım M (2012) Patates böceği (leptinotarsa decemlineata say.)’Ne dayanikli bitkiler elde etmek amaciyla patates (solanum tuberosum L.)’In genetik transformasyonu. Çukurova Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, pp 27–33Google Scholar
  48. Zhou Z, Pang J, Guo W, Zhong N, Tian Y, Xia G, Wu J (2012) Evaluation of the resistance of transgenic potato plants expressing various levels of Cry3A against the Colorado potato beetle (Leptinotarsa decemlineata Say) in the laboratory and field. Pest Manag Sci 68:1595–1604CrossRefGoogle Scholar
  49. Zhu JQ, Liu S, Ma J, Zhang JQ, Qi HS, Wei ZJ, Yao Q, Zhang WQ, Li S (2012) Improvement of pest resistance in transgenic tobacco plants expressing dsRNA of an insect-associated gene EcR. PLoS One 7:e38572  https://doi.org/10.1371/journal.pone.0038572 CrossRefPubMedPubMedCentralGoogle Scholar
  50. Zimdahl RL (2007) Fundamental of weed science, 3rd ed. Academic Press, LondonGoogle Scholar

Copyright information

© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and TechnologiesNigde Omer Halisdemir UniversityNigdeTurkey

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