Molecular analysis of mutant granule-bound starch synthase-I (waxy1) gene in diverse waxy maize inbreds
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Waxy corn is popular beacuse of its high amylopectin due to mutation in granule-bound starch synthase-I or Waxy1 (Wx1) gene. Here, we characterized the wx1 allele among 24 diverse waxy inbreds using gene-based markers. A total of 29 alleles with average of 1.81 alleles/locus were observed. Major allele frequency varied from 0.42 to 1.00, with mean of 0.74. The polymorphism information content ranged from 0.00 to 0.56 (average 0.24). Three simple sequence repeat markers, viz., phi027, phi022 and phi061 were more polymorphic in the study. The mean heterozygosity was 0.04, which indicated attainment of higher levels of homozygosity. Dissimilarity coefficient varied from 0.00 to 0.90 with average of 0.51. Seventeen diverse haplotypes of wx1 allele were observed that was consistent with the pedigree. Cluster analyses grouped 24 genotypes into two main clusters each having sub-clusters. The information generated here possesses great potential for improvement of high amylopectin in maize through marker-assisted selection. This is the first report of molecular dissection of wx1 gene among the novel waxy inbreds developed in India.
KeywordsAmylopectin Characterization Haplotypes Maize Waxy inbreds
We are grateful to ICAR-IARI, New Delhi, for funding the study. We also express thanks to Dr. B.M. Prasanna, CIMMYT for sharing the source waxy germplasm.
Design of experiment: FH; development of waxy inbreds: FH; compilation of marker information from public domain: SKJ; genotyping: RC and ELD; data analysis: FH and RC; field evaluation and maintenance of lines: RUZ; drafting of manuscript: FH and VM.
Compliance with ethical standards
Conflict of interest
The authors declare that no conflict of interest exists.
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