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3 Biotech

, 8:505 | Cite as

Decolorization and detoxification of textile wastewaters by recombinant Myceliophthora thermophila and Trametes trogii laccases

  • Klára Herkommerová
  • Jiří Dostál
  • Iva Pichová
Original Article
  • 37 Downloads

Abstract

Laccases are multi-copper oxidoreductases with broad biotechnological applications. Here, we report detailed biochemical characterization of purified recombinant laccases originating from Myceliophthora thermophila (MtL) and Trametes trogii (TtL). We identified optimal conditions for decolorization of commercial dyes and textile wastewater samples. We also tested the toxicity of decolorized wastewater samples using human peripheral blood mononuclear cells. MtL and TtL were expressed in Saccharomyces cerevisiae, and secreted enzymes were purified by consecutive hydrophobic and gel chromatography. The molecular masses of TtL (~ 65 kDa) and MtL (> 100 kDa) suggested glycosylation of the recombinant enzymes. Deglycosylation of MtL and TtL led to 25% and 10% decreases in activity, respectively. In a thermal stability assay, TtL retained 61% and MtL 86% of the initial activity at 40 °C. While TtL retained roughly 50% activity at 60 °C, MtL lost stability at temperatures higher than 40 °C. MtL and TtL preferred syringaldazine as a substrate, and the catalytic efficiencies for ABTS oxidation were 7.5 times lower than for syringaldazine oxidation. In the presence of the mediator HBT, purified TtL almost completely decolorized dyes within 30 min and substantially decolorized wastewater samples from a textile factory (up to 74%) within 20 h. However, products of TtL-catalyzed decolorization were more toxic than MtL-decolorized products, which were almost completely detoxified.

Keywords

Laccases Textile dyes Wastewater Decolorization Toxicity PBMCs 

Notes

Acknowledgements

This work was supported by Grant NPU LO 1302 from the Ministry of Education, Youth and Sports of the Czech Republic. The authors thank Zdeněk Voburka for N-terminal protein sequencing, Martin Hubálek for performing the peptide mass fingerprinting analysis, Miroslav Hájek for the toxicity study, Petr Beier for a consultation in dye chemistry, Zuzana Antošová for a consultation of decolorization experiments and Radek Stloukal (Lentikat’s a.s., Straž pod Ralskem, Czech Republic) for providing samples of textile industry wastewater originating from CNM textile a.s. (Baška, Czech Republic.)

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13205_2018_1525_MOESM1_ESM.pdf (825 kb)
Supplementary material 1 (PDF 825 KB)

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Copyright information

© King Abdulaziz City for Science and Technology 2018

Authors and Affiliations

  1. 1.Laboratory of Viral and Microbial ProteinsInstitute of Organic Chemistry and Biochemistry of the Czech Academy of SciencesPragueCzech Republic
  2. 2.Department of Membrane TransportInstitute of Physiology of the Czech Academy of SciencePragueCzech Republic

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